RESUMO
Foxp3-expressing regulatory T cells (Tregs) are central regulators of immune homeostasis and tolerance. As it has been suggested that proper Treg function is compromised under inflammatory conditions, seeking for a pathway that enhances or stabilizes Treg function is a subject of considerable interest. We report that interleukin (IL)-27, an IL-12 family cytokine known to have both pro- and anti-inflammatory roles in T cells, plays a pivotal role in enhancing Treg function to control T cell-induced colitis, a model for inflammatory bowel disease (IBD) in humans. Unlike wild-type (WT) Tregs capable of inhibiting colitogenic T-cell expansion and inflammatory cytokine expression, IL-27R-deficient Tregs were unable to downregulate inflammatory T-cell responses. Tregs stimulated with IL-27 expressed substantially improved suppressive function in vitro and in vivo. IL-27 stimulation of Tregs induced expression of Lag3, a surface molecule implicated in negatively regulating immune responses. Lag3 expression in Tregs was critical to mediate Treg function in suppressing colitogenic responses. Human Tregs also displayed enhanced suppressive function and Lag3 expression following IL-27 stimulation. Collectively, these results highlight a novel function for the IL-27/Lag3 axis in modulating Treg regulation of inflammatory responses in the intestine.
Assuntos
Antígenos CD/imunologia , Colite/imunologia , Fatores de Transcrição Forkhead/imunologia , Interleucinas/imunologia , Receptores de Interleucina/imunologia , Linfócitos T Reguladores/imunologia , Animais , Antígenos CD/genética , Proliferação de Células , Colite/genética , Colite/patologia , Modelos Animais de Doenças , Fatores de Transcrição Forkhead/genética , Regulação da Expressão Gênica , Humanos , Interleucinas/genética , Interleucinas/farmacologia , Ativação Linfocitária , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Cultura Primária de Células , Receptores de Interleucina/deficiência , Receptores de Interleucina/genética , Transdução de Sinais , Linfócitos T Reguladores/efeitos dos fármacos , Linfócitos T Reguladores/patologia , Proteína do Gene 3 de Ativação de LinfócitosRESUMO
CD4+ T cell proliferation depends on the balance between NO and extra-cellular superoxide (O2-). By reducing NO bio-availability, O2- promotes splenic T cell proliferation and immune response intensity. Here, we show that spleen cells from naïve mice produced neither NO nor O2- during T cell activation, but Gr-1+ splenocytes from primed mice regulated Ag-specific T cell expansion via production of both molecules. Purified splenic Gr-1+ cells included mostly granulocytes at various stages of maturation, as well as monocytes. Activation or recruitment of regulatory Gr-1+ cells was dependent on immunization with CFA. Importantly, these regulatory cells were not detected in draining lymph nodes. These data suggest that innate Gr-1+ splenic cells regulate adaptive immunity.
Assuntos
Proliferação de Células , Receptores de Quimiocinas/biossíntese , Baço/citologia , Linfócitos T/citologia , Linfócitos T/imunologia , Animais , Adjuvante de Freund , Fatores Imunológicos/biossíntese , Fatores Imunológicos/fisiologia , Lipídeos , Ativação Linfocitária/imunologia , Complexo Principal de Histocompatibilidade/fisiologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Mycobacterium tuberculosis/imunologia , Óxido Nítrico/biossíntese , Óxido Nítrico/fisiologia , Fase de Repouso do Ciclo Celular/imunologia , Baço/imunologia , Baço/metabolismo , Superóxidos/metabolismo , Superóxidos/farmacologia , Linfócitos T/metabolismoRESUMO
Staphylococcal enterotoxin (SE) B and seven other staphylococcal superantigens (SAg), despite promoting vigorous Ig production in human peripheral blood mononuclear cell cultures, are exceedingly poor at eliciting Ig responses in cultures of spleen cells from C57BL/10J (B10) or C3H/HeJ mice. In contrast, SEB elicits Ig responses in cultures of spleen cells from human MHC class II-transgenic mice. Whereas i.p. administration of SEB (0.2-20 microg) to non-transgenic B10 mice elicits very weak in vivo Ig responses, identical treatment of CD4(+) cell-intact (but not CD4(+) cell-depleted) human MHC class II-transgenic mice elicits dramatic increases in both splenic Ig-secreting cells and serum Ig levels. Over a 2-week period, the SEB-induced in vivo Ig responses peak and then plateau or fall in association with a preferential increase in splenic CD8(+) cells. Nevertheless, in vivo depletion of CD8(+) cells has no sustained effect on SEB-driven Ig responses. Taken together, these observations demonstrate that the effects of SAg on in vivo humoral immune responses are highly CD4(+) cell dependent, are substantially CD8(+) cell independent and can be successfully investigated using human MHC class II-transgenic mice. This model system may be useful in investigating the polyclonally activating effects of microbial products (prototypic environmental insults) on the development of systemic autoimmunity.
Assuntos
Antígenos de Bactérias/imunologia , Linfócitos T CD4-Positivos/imunologia , Antígenos de Histocompatibilidade Classe II/imunologia , Staphylococcus aureus/imunologia , Superantígenos/imunologia , Animais , Autoimunidade , Linfócitos T CD8-Positivos , Enterotoxinas/imunologia , Genes Codificadores da Cadeia beta de Receptores de Linfócitos T , Antígenos HLA-DQ/genética , Antígeno HLA-DR2/genética , Antígenos de Histocompatibilidade Classe II/genética , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Depleção Linfocítica , Camundongos , Camundongos Endogâmicos , Camundongos Transgênicos , Fragmentos de Peptídeos , Receptores de Antígenos de Linfócitos T alfa-beta , Especificidade da Espécie , Baço/citologia , Baço/imunologiaRESUMO
CD8(+) T cells are required to control acute viral replication in the CNS following infection with neurotropic coronavirus. By contrast, studies in B cell-deficient (muMT) mice revealed Abs as key effectors in suppressing virus recrudescence. The apparent loss of initial T cell-mediated immune control in the absence of B cells was investigated by comparing T cell populations in CNS mononuclear cells from infected muMT and wild-type mice. Following viral recrudescence in muMT mice, total CD8(+) T cell numbers were similar to those of wild-type mice that had cleared infectious virus; however, virus-specific T cells were reduced at least 3-fold by class I tetramer and IFN-gamma ELISPOT analysis. Although overall T cell recruitment into the CNS of muMT mice was not impaired, discrepancies in frequencies of virus-specific CD8(+) T cells were most severe during acute infection. Impaired ex vivo cytolytic activity of muMT CNS mononuclear cells, concomitant with reduced frequencies, implicated IFN-gamma as the primary anti viral factor early in infection. Reduced virus-specific CD8(+) T cell responses in the CNS coincided with poor peripheral expansion and diminished CD4(+) T cell help. Thus, in addition to the lack of Ab, limited CD8(+) and CD4(+) T cell responses in muMT mice contribute to the ultimate loss of control of CNS infection. Using a model of virus infection restricted to the CNS, the results provide novel evidence for a role of B cells in regulating T cell expansion and differentiation into effector cells.
Assuntos
Linfócitos B/imunologia , Infecções por Coronavirus/imunologia , Encefalite Viral/imunologia , Linfopenia/imunologia , Subpopulações de Linfócitos T/imunologia , Doença Aguda , Animais , Linfócitos B/patologia , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD4-Positivos/patologia , Linfócitos T CD8-Positivos/imunologia , Linfócitos T CD8-Positivos/patologia , Linfócitos T CD8-Positivos/virologia , Divisão Celular/genética , Divisão Celular/imunologia , Infecções por Coronavirus/genética , Encefalite Viral/genética , Encefalite Viral/patologia , Epitopos de Linfócito T/imunologia , Ativação Linfocitária/genética , Contagem de Linfócitos , Linfopenia/genética , Linfopenia/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Mutantes , Vírus da Hepatite Murina/imunologia , Subpopulações de Linfócitos T/patologia , Subpopulações de Linfócitos T/virologiaRESUMO
Cells expressing the spike (S) glycoprotein of the neurotropic JHM strain (JHMV) of mouse hepatitis virus (MHV) are susceptible to lysis by B cells derived from naïve mice, including B cells from perforin-deficient mice. Cytolysis requires interaction of the virus receptor and the viral S glycoprotein, is independent of other viral-induced components, and is not a unique property of B cells. Neutralizing anti-S-protein monoclonal antibodies (mAb) and a mAb specific for the viral receptor inhibit lysis. However, cells infected with an MHV strain unable to induce cell-cell fusion are resistant to lysis and lysis of JHMV-infected cells is inhibited by an anti-S-protein nonneutralizing mAb which prevents S-protein-mediated cell fusion. These data suggest that B cells may function as antibody-independent innate immune response during JHMV infection in vivo.
Assuntos
Linfócitos B/imunologia , Infecções por Coronavirus/imunologia , Vírus da Hepatite Murina/imunologia , Animais , Comunicação Celular/imunologia , Infecções por Coronavirus/virologia , Citotoxicidade Imunológica , Imunidade Inata , Camundongos , Sistema Nervoso/virologiaRESUMO
Mice infected with neurotropic strains of mouse hepatitis virus (MHV) clear infectious virus; nevertheless, viral persistence in the central nervous system (CNS) is associated with ongoing primary demyelination. Acute infection induces a potent regional CD8+ T-cell response. The high prevalence of virus specific T cells correlates with ex vivo cytolytic activity, interferon-gamma (IFN-gamma) secretion and efficient reduction in virus. Viral clearance from most cell types is controlled by a perforin dependent mechanism. However, IFN-gamma is essential for controlling virus replication in oligodendrocytes. Furthermore, CD4+ T cells enhance CD8+ T-cell survival and effectiveness. Clearance of infectious virus is associated with a gradual decline of CNS T cells; nevertheless, activated T cells are retained within the CNS. The loss of cytolytic activity, but retention of IFN-gamma secretion during viral clearance suggests stringent regulation of CD8+ T-cell effector function, possibly as a means to minimize CNS damage. However, similar CD8+ T-cell responses to demyelinating and non demyelinating JHMV variants support the notion that CD8+ T cells do not contribute to the demyelinating process. Although T-cell retention is tightly linked to the presence of persisting virus, contributions to regulating the latent state are unknown. Studies in B-cell-deficient mice suggest that antibodies are required to prevent virus recrudescence. Although acute JHMV infection is thus primarily controlled by CD8+ T cells, both CD4+ T cells and B cells make significant contributions in maintaining the balance between viral replication and immune control, thus allowing host and pathogen survival.
Assuntos
Infecções do Sistema Nervoso Central/imunologia , Infecções por Coronavirus/imunologia , Vírus da Hepatite Murina/imunologia , Animais , Infecções do Sistema Nervoso Central/virologia , Camundongos , Camundongos Endogâmicos BALB CRESUMO
Viral induced demyelination, in both humans and rodent models, has provided unique insights into the cell biology of oligodendroglia, their complex cell-cell interactions and mechanisms of myelin destruction. They illustrate mechanisms of viral persistence, including latent infections in which no infectious virus is readily evident, virus reactivation and viral-induced tissue damage. These studies have also provided excellent paradigms to study the interactions between the immune system and the central nervous system (CNS). Although of interest in their own right, an understanding of the diverse mechanisms used by viruses to induce demyelination may shed light into the etiology and pathogenesis of the common demyelinating disorder multiple sclerosis (MS). This notion is supported by the persistent view that a viral infection acquired during adolescence might initiate MS after a long period of quiescence. Demyelination in both humans and rodents can be initiated by infection with a diverse group of enveloped and non-enveloped RNA and DNA viruses (Table 1). The mechanisms that ultimately result in the loss of CNS myelin appear to be equally diverse as the etiological agents capable of causing diseases which result in demyelination. Although demyelination can be a secondary result of axonal loss, in many examples of viral induced demyelination, myelin loss is primary and associated with axonal sparing. This suggests that demyelination induced by viral infections can result from: 1) a direct viral infection of oligodendroglia resulting in cell death with degeneration of myelin and its subsequent removal; 2) a persistent viral infection, in the presence or absence of infectious virus, resulting in the loss of normal cellular homeostasis and subsequent oligodendroglial death; 3) a vigorous virus-specific inflammatory response wherein the virus replicates in a cell type other than oligodendroglia, but cytokines and other immune mediators directly damage the oligodendroglia or the myelin sheath; or 4) infection initiates activation of an immune response specific for either oligodendroglia or myelin components. Virus-induced inflammation may be associated with the processing of myelin or oligodendroglial components and their presentation to the host's own T cell compartment. Alternatively, antigenic epitopes derived from the viral proteins may exhibit sufficient homology to host components that the immune response to the virus activates autoreactive T cells, i.e. molecular mimicry. Although it is not clear that each of these potential mechanisms participates in the pathogenesis of human demyelinating disease, analysis of the diverse demyelinating viral infections of both humans and rodents provides examples of many of these potential mechanisms.
Assuntos
Infecções do Sistema Nervoso Central/virologia , Doenças Desmielinizantes/virologia , Oligodendroglia/fisiologia , Viroses/fisiopatologia , Animais , Morte Celular , Infecções do Sistema Nervoso Central/fisiopatologia , Doenças Desmielinizantes/fisiopatologia , Humanos , Leucoencefalopatia Multifocal Progressiva/fisiopatologia , Camundongos , Modelos Animais , Vírus da Hepatite Murina , Oligodendroglia/virologia , Panencefalite Esclerosante Subaguda/fisiopatologia , Theilovirus , Viroses/imunologiaAssuntos
Linfócitos T CD8-Positivos/imunologia , Viroses do Sistema Nervoso Central/imunologia , Viroses do Sistema Nervoso Central/virologia , Vírus da Hepatite Murina/imunologia , Vírus da Hepatite Murina/patogenicidade , Animais , Infecções por Coronavirus/imunologia , Infecções por Coronavirus/virologia , Humanos , CamundongosAssuntos
Encefalomielite/imunologia , Regulação da Expressão Gênica , Metaloproteinases da Matriz/metabolismo , Vírus da Hepatite Murina/imunologia , Inibidores Teciduais de Metaloproteinases/metabolismo , Animais , Infecções por Coronavirus/imunologia , Infecções por Coronavirus/virologia , Encefalomielite/virologia , Masculino , Metaloproteinases da Matriz/genética , Camundongos , Camundongos Endogâmicos BALB C , Inibidores Teciduais de Metaloproteinases/genéticaAssuntos
Linfócitos T CD8-Positivos/imunologia , Viroses do Sistema Nervoso Central/imunologia , Sistema Nervoso Central/virologia , Interferon gama/biossíntese , Vírus da Hepatite Murina/imunologia , Transferência Adotiva , Animais , Linfócitos T CD8-Positivos/metabolismo , Linfócitos T CD8-Positivos/transplante , Viroses do Sistema Nervoso Central/virologia , Infecções por Coronavirus/imunologia , Infecções por Coronavirus/virologia , Interferon gama/deficiência , Camundongos , Camundongos Endogâmicos BALB C , Camundongos SCID , Vírus da Hepatite Murina/fisiologia , Replicação ViralAssuntos
Encéfalo/imunologia , Viroses do Sistema Nervoso Central/imunologia , Vírus da Hepatite Murina/imunologia , Medula Espinal/imunologia , Linfócitos T Citotóxicos/imunologia , Doença Aguda , Animais , Encéfalo/virologia , Viroses do Sistema Nervoso Central/virologia , Infecções por Coronavirus/imunologia , Infecções por Coronavirus/virologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Vírus da Hepatite Murina/fisiologia , Medula Espinal/virologiaAssuntos
Linfócitos B/imunologia , Infecções por Coronavirus/imunologia , Citotoxicidade Imunológica , Vírus da Hepatite Murina/imunologia , Animais , Moléculas de Adesão Celular , Comunicação Celular , Linhagem Celular , Testes Imunológicos de Citotoxicidade , Glicoproteínas/metabolismo , Células HeLa , Humanos , Glicoproteínas de Membrana/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Vírus da Hepatite Murina/patogenicidade , Glicoproteína da Espícula de Coronavírus , Proteínas do Envelope Viral/metabolismoRESUMO
Multiple sclerosis, an inflammatory, demyelinating disease of the CNS currently lacks an effective therapy. We show here that CNS inflammation and clinical disease in experimental autoimmune encephalomyelitis, an experimental model of multiple sclerosis, could be prevented completely by a replication-defective adenovirus vector expressing the anti-inflammatory cytokine IL-10 (replication-deficient adenovirus expressing human IL-10), but only upon inoculation into the CNS where local infection and high IL-10 levels were achieved. High circulating levels of IL-10 produced by i. v. infection with replication-deficient adenovirus expressing human IL-10 was ineffective, although the immunological pathways for disease are initiated in the periphery in this disease model. In addition to this protective activity, intracranial injection of replication-deficient adenovirus expressing human IL-10 to mice with active disease blocked progression and accelerated disease remission. In a relapsing-remitting disease model, IL-10 gene transfer during remission prevented subsequent relapses. These data help explain the varying outcomes previously reported for systemic delivery of IL-10 in experimental autoimmune encephalomyelitis and show that, for optimum therapeutic activity, IL-10 must either access the CNS from the peripheral circulation or be delivered directly to it by strategies including the gene transfer described here.
Assuntos
Encéfalo/imunologia , Encéfalo/metabolismo , Encefalomielite Autoimune Experimental/imunologia , Encefalomielite Autoimune Experimental/prevenção & controle , Interleucina-10/genética , Medula Espinal/imunologia , Medula Espinal/metabolismo , Adenoviridae/genética , Adjuvantes Imunológicos/administração & dosagem , Adjuvantes Imunológicos/genética , Animais , Encéfalo/patologia , Modelos Animais de Doenças , Encefalomielite Autoimune Experimental/genética , Encefalomielite Autoimune Experimental/patologia , Técnicas de Transferência de Genes , Vetores Genéticos/administração & dosagem , Vetores Genéticos/imunologia , Injeções Intravenosas , Injeções Intraventriculares , Injeções Subcutâneas , Interleucina-10/administração & dosagem , Interleucina-10/biossíntese , Interleucina-10/fisiologia , Camundongos , Camundongos Endogâmicos BALB C , Esclerose Múltipla/genética , Esclerose Múltipla/imunologia , Especificidade de Órgãos/genética , Especificidade de Órgãos/imunologia , Prevenção SecundáriaRESUMO
The continued presence of virus-specific CD8(+) T cells within the central nervous system (CNS) following resolution of acute viral encephalomyelitis implicates organ-specific retention. The role of viral persistence in locally maintaining T cells was investigated by infecting mice with either a demyelinating, paralytic (V-1) or nonpathogenic (V-2) variant of a neurotropic mouse hepatitis virus, which differ in the ability to persist within the CNS. Class I tetramer technology revealed more infiltrating virus-specific CD8(+) T cells during acute V-1 compared to V-2 infection. However, both total and virus-specific CD8(+) T cells accumulated at similar peak levels in spinal cords by day 10 postinfection (p.i.). Decreasing viral RNA levels in both brains and spinal cords following initial virus clearance coincided with an overall progressive loss of both total and virus-specific CD8(+) T cells. By 9 weeks p.i., T cells had largely disappeared from brains of both infected groups, consistent with the decline of viral RNA. T cells also completely disappeared from V-2-infected spinal cords coincident with the absence of viral RNA. By contrast, a significant number of CD8(+) T cells which contained detectable viral RNA were recovered from spinal cords of V-1-infected mice. The data indicate that residual virus from a primary CNS infection is a vital component in mediating local retention of both CD8(+) and CD4(+) T cells and that once minimal thresholds of stimuli are lost, T cells within the CNS cannot survive in an autonomous fashion.
Assuntos
Encéfalo/virologia , Linfócitos T CD8-Positivos/virologia , Infecções por Coronavirus/virologia , Vírus da Hepatite Murina/crescimento & desenvolvimento , Medula Espinal/virologia , Animais , Encéfalo/imunologia , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD8-Positivos/imunologia , Citometria de Fluxo , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Especificidade de Órgãos , RNA Viral/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Medula Espinal/imunologiaRESUMO
Female SJL mice preferentially mount Th1-immune responses and are susceptible to the active induction of experimental allergic encephalomyelitis. By contrast, young adult male SJL are resistant to experimental allergic encephalomyelitis due to an APC-dependent induction of Th2 cells. The basis for this gender-dependent differential T cell induction was examined by analysis of macrophage APC cytokine secretion during T cell activation. APC derived from females secrete IL-12, but not IL-10, during T cell activation. By contrast, APC derived from males secrete IL-10, but not IL-12, during T cell activation. Activation of T cells with APC derived from the opposite sex demonstrated that these cytokines were derived from the respective APC populations. Furthermore, inhibition of IL-10, but not TGF-beta, during T cell activation resulted in the secretion of IL-12 by male-derived APC. APC from naive male mice, in which IL-10 was reduced in vivo before isolation, also secrete IL-12, demonstrating altered APC cytokine secretion was due to an environment high in IL-10 before Ag encounter. Finally, APC derived from castrated male mice preferentially secrete IL-12 during T cell activation. These data demonstrate a link between gonadal hormones and APC activity and suggest that these hormones alter the APC, thereby influencing cytokine secretion during initial T cell activation.
Assuntos
Células Apresentadoras de Antígenos/imunologia , Células Apresentadoras de Antígenos/metabolismo , Interleucina-10/fisiologia , Interleucina-12/metabolismo , Caracteres Sexuais , Animais , Células Cultivadas , Feminino , Interleucina-12/antagonistas & inibidores , Ativação Linfocitária , Macrófagos Peritoneais/imunologia , Macrófagos Peritoneais/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos , Orquiectomia , Linfócitos T Auxiliares-Indutores/imunologiaRESUMO
The pathogenesis of the neurotropic strain of mouse hepatitis virus in Fas-deficient mice suggested that Fas-mediated cytotoxicity may be required during viral clearance after the loss of perforin-mediated cytotoxicity. The absence of both Fas- and perforin-mediated cytolysis resulted in an uncontrolled infection, suggesting a redundancy of cytolytic pathways to control virus replication.
Assuntos
Infecções do Sistema Nervoso Central/virologia , Infecções por Coronavirus/virologia , Glicoproteínas de Membrana/imunologia , Vírus da Hepatite Murina/patogenicidade , Receptor fas/imunologia , Animais , Encéfalo/patologia , Encéfalo/virologia , Linhagem Celular , Infecções do Sistema Nervoso Central/patologia , Infecções por Coronavirus/patologia , Proteína Ligante Fas , Glicoproteínas de Membrana/genética , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Perforina , Proteínas Citotóxicas Formadoras de Poros , Medula Espinal/patologia , Receptor fas/genéticaRESUMO
The nucleocapsid (N) protein of mouse hepatitis virus (MHV) is the major virion structural protein. It associates with both viral genomic RNA and subgenomic mRNAs and has structural and non-structural roles in replication including viral RNA-dependent RNA transcription, genome replication, encapsidation and translation. These processes all involve RNA-protein interactions between the N protein and viral RNAs. To better understand the RNA-binding properties of this multifunctional protein, the N protein was expressed in Escherichia coli as a chimeric protein fused to glutathione-S-transferase (GST). Biochemical analyses of RNA-binding properties were performed on full-length and partial N protein segments to define the RNA-binding domain. The full-length N protein and the GST-N protein fusion product had similar binding activities with a dissociation constant (K(d)) of 14 nM when the MHV 5'-leader sequence was used as ligand. The smallest N protein fragment which retained RNA-binding activity was a 55 aa segment containing residues 177-231 which bound viral RNA with a K(d) of 32 nM. A consensus viral sequence recognized by the N protein was inferred from these studies; AAUCYAAAC was identified to be the potential minimum ligand for the N protein. Although the core UCYAA sequence is often tandemly repeated in viral genomes, ligands containing one or more repeats of UCYAA showed no difference in binding to the N protein. Together these data demonstrate a high-affinity, specific interaction between the N protein and a conserved RNA sequence present at the 5'-ends of MHV mRNA.
Assuntos
Regiões 5' não Traduzidas/metabolismo , Vírus da Hepatite Murina/metabolismo , Nucleocapsídeo/metabolismo , RNA Mensageiro/metabolismo , RNA Viral/metabolismo , Regiões 5' não Traduzidas/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Sítios de Ligação , Sequência Conservada , Escherichia coli/genética , Escherichia coli/metabolismo , Glutationa Transferase/genética , Glutationa Transferase/metabolismo , Ligantes , Camundongos , Dados de Sequência Molecular , Vírus da Hepatite Murina/genética , Nucleocapsídeo/química , Nucleocapsídeo/genética , Proteínas do Nucleocapsídeo , RNA Viral/genética , Proteínas Recombinantes de Fusão/metabolismoRESUMO
Acute and chronic demyelination are hallmarks of CNS infection by the neurotropic JHM strain of mouse hepatitis virus. Although infectious virus is cleared by CD8+ T cells, both viral RNA and activated CD8+ T cells remain in the CNS during persistence potentially contributing to pathology. To dissociate immune from virus-mediated determinants initiating and maintaining demyelinating disease, mice were infected with two attenuated viral variants differing in a hypervariable region of the spike protein. Despite similar viral replication and tropism, one infection was marked by extensive demyelination and paralysis, whereas the other resulted in no clinical symptoms and minimal neuropathology. Mononuclear cells from either infected brain exhibited virus specific ex vivo cytolytic activity, which was rapidly lost during viral clearance. As revealed by class I tetramer technology the paralytic variant was superior in inducing specific CD8+ T cells during the acute disease. However, after infectious virus was cleared, twice as many virus-specific IFN-gamma-secreting CD8+ T cells were recovered from the brains of asymptomatic mice compared with mice undergoing demyelination, suggesting that IFN-gamma ameliorates rather than perpetuates JHM strain of mouse hepatitis virus-induced demyelination. The present data thus indicate that in immunocompetent mice, effector CD8+ T cells control infection without mediating either clinical disease or demyelination. In contrast, demyelination correlated with early and sustained infection of the spinal cord. Rapid viral spread, attributed to determinants within the spike protein and possibly perpetuated by suboptimal CD8+ T cell effector function, thus ultimately leads to the process of immune-mediated demyelination.
Assuntos
Linfócitos T CD8-Positivos/imunologia , Infecções por Coronavirus/imunologia , Infecções por Coronavirus/virologia , Doenças Desmielinizantes/imunologia , Doenças Desmielinizantes/virologia , Vírus da Hepatite Murina/patogenicidade , Animais , Linfócitos T CD8-Positivos/metabolismo , Doença Crônica , Infecções por Coronavirus/etiologia , Infecções por Coronavirus/patologia , Citotoxicidade Imunológica/imunologia , Doenças Desmielinizantes/etiologia , Doenças Desmielinizantes/patologia , Interferon gama/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Vírus da Hepatite Murina/imunologia , Paralisia/imunologia , Paralisia/virologia , Medula Espinal/imunologia , Medula Espinal/patologia , Medula Espinal/virologia , Carga Viral , Replicação Viral/imunologiaRESUMO
Microglia are important immunoregulatory cells within the central nervous system (CNS). Viral infection of primary microglia and splenic macrophage clones revealed that both exhibited a heterogeneous, but relatively low, sensitivity to cytolysis mediated by CD8(+) cytotoxic T lymphocytes (CTL). The majority of clones were poor in processing and presenting epitopes, despite triggering lysis when coated with peptide. These characteristics were retained by stable microglia lines. Reduced lysis did not correlate with class I expression and IFN-gamma treatment only partially enhanced recognition. In contrast, targeting the epitope into the endoplasmic reticulum restored cytolysis to levels achieved with exogenous peptide. An inherent resistance to cytolysis was revealed by efficient engagement of T cells in competition assays and the inability of saturating peptide to enhance cytolysis. These data suggest that microglia heterogeneity in antigen processing, in addition to low sensitivity to CTL lysis, contributes to limited CD8(+) T cell responses and viral CNS persistence.
Assuntos
Citotoxicidade Imunológica/imunologia , Antígenos H-2/biossíntese , Microglia/imunologia , Microglia/metabolismo , Linfócitos T Citotóxicos/imunologia , Linfócitos T Citotóxicos/metabolismo , Animais , Apresentação de Antígeno/imunologia , Linfócitos T CD8-Positivos/imunologia , Linhagem Celular , Células Clonais/imunologia , Testes Imunológicos de Citotoxicidade , Antígenos H-2/imunologia , Imunidade Inata/imunologia , Ativação Linfocitária/imunologia , Camundongos , Camundongos Endogâmicos BALB CRESUMO
Mouse hepatitis virus (MHV) strain JHM is a coronavirus that causes encephalitis and demyelination in susceptible rodents. The known receptors for MHV are all members of the carcinoembryonic antigen family. Although human forms of the MHV receptor can function as MHV receptors in some assays, no human cell line has been identified that can support wild-type MHV infection. Here we describe the infection of a human hepatocellular carcinoma cell line, HuH-7, with MHV. HuH-7 cells were susceptible to strains JHM-DL and JHM-DS, yielding virus titers nearly identical to those seen in mouse DBT cells. In contrast, HuH-7 cells were only marginally susceptible or completely resistant to infection by other MHV strains, including A59. JHM produced a strong cytopathic effect in HuH-7 cells with the formation of round plaques. Studies of various recombinant viruses between JHM and A59 strains suggested that the ability of JHM to infect HuH-7 cells was determined by multiple viral genetic elements. Blocking the viral spike (S) protein with a neutralizing antibody or a soluble form of the MHV receptor inhibited infection of HuH-7 cells, suggesting that infection is mediated through the S protein. Transfection with the prototype mouse receptor, biliary glycoprotein, rendered HuH-7 cells susceptible to infection by other MHV strains as well, suggesting that JHM uses a receptor distinct from the classical MHV receptor to infect HuH-7 cells. Possible implications for human disease are discussed.