Survival of Escherichia coli O157:H7 during the manufacture and ripening of Cacioricotta goat cheese.

The aim of this study was to assess the growth and survival of Escherichia coli O157:H7 during the manufacturing and ripening of Cacioricotta goat cheese. Goat milk was artificially contaminated with E. coli O157:H7 and the bacterial load was monitored from production up to 90 days of ripening. Goat milk was inoculated with 102 cfu ml-1 of E. coli O157:H7 and the bacterial count of the curd at time zero was 2.31 log10 cfu g-1. During the first day of ripening, the bacterial load has increased to 5.73 log10 cfu g-1 to more than 6.20 log10 cfu g-1 during the first week. The bacterial load remained constant up to 28 days and then slightly decreased until the end of ripening, with values of aw and pH of 0.88 and 5.41 respectively. The results of this study highlighted that E. coli O157:H7 is able to survive the manufacturing process and they suggest that the 90-day period of ripening alone is insufficient to remove E. coli O157:H7 in contaminated Cacioricotta goat cheese. Moreover, these results support the assumption that the presence of a low contamination of milk with E. coli O157:H7 could represent a potential source of infection and a threat to consumers.

Finding the elusive E×B staircase in magnetized plasmas.

Turbulence in hot magnetized plasmas is shown to generate permeable localized transport barriers that globally organize into the so-called "ExB staircase" [G. Dif-Pradalier et al., Phys. Rev. E, 82, 025401(R) (2010)]. Its domain of existence and dependence with key plasma parameters is discussed theoretically. Based on these predictions, staircases are observed experimentally in the Tore Supra tokamak by means of high-resolution fast-sweeping X-mode reflectometry. This observation strongly emphasizes the critical role of mesoscale self-organization in plasma turbulence and may have far-reaching consequences for turbulent transport models and their validation.

Levels and congener profiles of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs) in sheep milk from an industrialised area of Sardinia, Italy.

Concentrations of 7 polychlorinated dibenzo-p-dioxins (PCDDs), 10 polychlorinated dibenzofurans (PCDFs) and 22 polychlorinated biphenyls (PCBs), including 12 dioxin like-PCBs (non- and mono-ortho PCBs) were measured in 80 sheep milk samples from farms located in an industrialized area of Sardinia, Italy. PCDDs and PCDFs mean concentrations were 2.45 and 3.69 pgg(-1) fat basis, respectively. The mean dl-PCB concentration was 2.01 ngg(-1) fat basis, while cumulative ndl-PCB levels ranged from 1.02 to 20.42, with a mean of 4.92 ngg(-1) fat. The results expressed in pg WHO-TEQ/g fat showed that contamination level of milk was below the limit values for human consumption established by EC legislation. In the same way, all the investigated milk exhibited PCDD/Fs concentrations below EU action levels, while dl-PCBs concentrations exceeded the action level of 2.0 pg WHO-TEQ/g fat. These findings point to the need to continue to conduct general monitoring programmes, including also milk samples from areas not close to the contaminant-emitting industries, in order to better evaluate the impact of industrial activities on surrounding environment.

Occurrence of heavy metals (Hg, Cd, and Pb) and polychlorinated biphenyls in salted anchovies.

The most popular brands of salted anchovies (Engraulis encrasicolus) from the Mediterranean Sea and Atlantic Ocean were purchased from several Italian supermarkets and grocery stores. Heavy metal (Hg, Cd, and Pb) and polychlorinated biphenyl (PCB) levels were determined and assessed by comparing the concentrations in these samples with the maximum permissible limits set by the European Union (Reg EC 629/2008 and Reg EC 1881/2006 [Off. J. Eur. Union L 173:3-9 and 364:5-24, respectively]). The Hg and Cd levels were higher than those of Pb in all samples examined. For Hg and Pb, the concentrations recorded in this study were below the authorized limits, while an appreciable percentage of samples from both locations (Mediterranean Sea, 35%, and Atlantic Ocean, 25%) showed Cd levels exceeding the threshold recommended for human consumption. Concerning PCBs, the results of principal component analysis showed that samples from the two different marine areas appeared to be discriminate, with Mediterranean anchovies more contaminated than the others, in spite of their lower lipid content. However, anchovy samples from both locations had dioxinlike-PCB levels (Mediterranean Sea, 0.011 pg World Health Organization toxic equivalency [WHO-TEQ] g(-1), wet weight, and Atlantic Ocean, 0.007 pg WHO-TEQ g(-1), wet weight) that were below the WHO-TEQ maximum concentration set by European regulation. The results of this study will help in generating data needed for the assessment of heavy metal and PCB intake from this food.

Total and subcellular distribution of trace elements (Cd, Cu and Zn) in the liver and kidney of green turtles (Chelonia mydas) from the Mediterranean Sea.

This study investigated the subcellular distribution of Cd, Cu and Zn in liver and kidney of green turtles (Chelonia mydas) stranded along the Italian coast of the South Adriatic Sea (Eastern Mediterranean). Cd and Zn mean concentrations did not differ significantly between liver (4.26microgg(-1) and 34.53microgg(-1), respectively) and kidney (5.06microgg(-1) and 26.39microgg(-1), respectively), whereas the levels of Cu were significantly higher in liver (32.75microgg(-1)) than in kidney (8.20microgg(-1)) (p<0.009). Most of Cd, Cu and Zn was present in hepatic and renal cytosol, and their concentrations increased with total levels in both organs, indicating that cytosol has a crucial role in metal accumulation. Cd and Cu in hepatic and renal cytosol were present mostly in metallothionein fractions (MTs), whereas Zn was fractionated into MTs and high-molecular-weight-substances (HMWS). The comparison with the results of other investigations on individuals of the same species collected in different marine areas shows good agreement relatively to essential metals. For Cd our data are comparable with those encountered in specimens from the Mediterranean Sea (Cyprus) confirming the homogeneity of the area comprising the south-eastern basin of the Mediterranean Sea from an ecological point of view.

Cadmium and mercury in cephalopod molluscs: estimated weekly intake.

Cadmium and mercury concentrations were measured in the flesh and hepatopancreas of different species of cephalopod molluscs (European squid, common octopus, curled octopus, horned octopus, pink cuttlefish, common cuttlefish) in order to establish whether the concentrations exceeded the maximum levels fixed by the European Commission. In hepatopancreas, the levels of cadmium were substantially higher than those in flesh (flesh 0.11-0.87 microg g(-1) wet weight, hepatopancreas 2.16-9.39 microg g(-1) wet weight), whilst the levels of mercury (flesh 0.13-0.55 microg g(-1) wet weight, hepatopancreas 0.23-0.79 microg g(-1)) were approximately double those in flesh. Concentrations exceeding the maximum permitted limit of cadmium were found in 39.8 and 41.0% of common octopus and pink cuttlefish flesh, respectively. For mercury, concentrations above the limit were found only in octopuses, and precisely in 36.8, 50.0 and 20.0% of flesh samples of common, curled and horned octopus, respectively. In the hepatopancreas, concentrations of cadmium and mercury were above the proposed limits in all the samples examined. The estimated weekly intake of between 0.09 and 0.49 microg kg(-1) body weight for cadmium and between 0.05 and 0.24 microg kg(-1) body weight for mercury made only a small contribution to the provisional tolerable weekly intake (cadmium 1.3-7.0%, mercury 1.0-4.8%) set by the WHO.

Trace elements in loggerhead turtles (Caretta caretta) from the eastern Mediterranean Sea: overview and evaluation.

Concentrations of trace elements (Hg, Cd, Pb, Zn, Cu, Fe, and Se) in different organs and tissues (liver, kidney, muscle tissue, spleen, heart, lung, and fat tissue) of loggerhead turtles Caretta caretta from eastern Mediterranean Sea were determined. The highest levels of mercury and cadmium were found in liver (Hg: 0.43 microg g(-1) wet weight; Cd: 3.36 microg g(-1) wet weight) and kidney (Hg: 0.16 microg g(-1) wet weight; Cd: 8.35 microg g(-1) wet weight). For lead the overall concentrations were low and often below the limit of detection. Copper and selenium tended to be higher in liver than in other tissues and organs, while for zinc the concentrations were quite homogenous in the different organs and tissues, except fat tissue (64.7 microg g(-1) wet weight) which showed a higher accumulation of this element. For iron the greatest concentrations were observed in liver (409 microg g(-1) wet weight) and spleen (221 microg g(-1) wet weight).

Polychlorinated biphenyl residues in deep-sea fish from Mediterranean Sea.

Polychlorinated biphenyls including coplanar congeners were measured in the liver of different species of skates and ghostsharks. The mean concentrations of PCBs in skates (314 ng/g wet wt) were comparable with those in ghostsharks (285 ng/g wet wt). The specific analysis of PCB congeners in skates hepatic tissue revealed a profile dominated by hexa-, penta-, and heptachlorinated congeners followed by trichlorobiphenyls, tetra- and decachlorobiphenyls. A different profile dominated by hexa-, tri-, and pentachlorinated congeners, followed by heptachlorobiphenyl and decachlorobiphenyls was found in ghostsharks liver. The estimated 2,3,7,8-tetrachlorodibenzo-p-dioxin toxic equivalents (TEQs) of coplanar PCBs in skates and ghostsharks were 0.48 and 0.33 pg/g wet weight, respectively, with mono-ortho congeners PCB105, 118 and 156 contributing more than 90% to the TEQs.

Total mercury and methylmercury content in edible fish from the Mediterranean Sea.

The objective of this study was to determine the current levels of total mercury and methylmercury in the muscle tissues of different fish species caught in the Mediterranean Sea to ascertain whether these concentrations exceed the maximum level stipulated by the European Commission Decision. Total mercury concentrations in the muscles of skates ranged from 0.18 to 1.85 mg/kg (wet weight) (average, 1.00 mg/kg) while levels of 0.11 to 1.92 mg/kg (wet weight) (average, 0.70 mg/kg) and 0.21 to 1.74 mg/kg (wet weight) (average, 0.70 mg/kg) were recorded for blue whiting and red mullet, respectively. For 66.7% of long nose skate samples, 61.4% of thornback ray samples, 42.8% of winter skate samples, and 38% of starry ray samples, the total mercury concentrations exceeded the prescribed legal limit (1.0 mg/kg [wet weight]). Concentrations exceeding the maximum total mercury level stipulated by the European Commission Decision (0.5 mg/kg [wet weight]) were observed in 63.6 and 40% of blue whiting and striped mullet samples, respectively. Mercury was present in the different species almost completely in the methylated form at 55 to 100%. Weekly intakes were estimated and compared with the provisional tolerable weekly intake recommended by the Joint Food and Agriculture Organization/World Health Organization Expert Committee on Food Additives.

Survey of total mercury and methylmercury levels in edible fish from the Adriatic Sea.

Total mercury and methylmercury concentrations were measured in the muscle tissue of different fish species from the Adriatic Sea to ascertain whether the concentrations exceeded the maximum level fixed by the European Commission. Large species-dependent variability was observed. The highest total mercury mean concentrations were in benthic (0.20-0.76 microg g(-1) wet wt) and demersal fish (0.22-0.73 microg g(-1) wet wt), while pelagic species showed the lowest levels (0.09-0.23 microg g(-1) wet wt). In 15% of frost fish, in 42% of skate and in 30% of angler fish samples total mercury concentrations exceeded the maximum level fixed by the European Commission (Hg = 1 microg g(-1) wet wt); for the species for which the maximum level was set to 0.5 microg g(-1) wet wt, concentrations exceeding the prescribed legal limit were observed in 6.4% of bokkem, in 6.6% of pandora, in 20% of megrin, in 12.5% of four-spotted megrim, in 16% of striped mullet, in 5.0% of forkbeard and in 5.3% of picarel samples. In all the different species, mercury was present almost completely in the methylated form, with mean percentages between 70 and 100%. Weekly intake was estimated and compared with the provisional tolerable weekly intake recommended by the FAO/WHO Expert Committee on Food Additives. A high exposure was associated with the consumption of only skates, frost fish and angler fish, thought the consumption of the other species, such as, megrim, four spotted megrim, red fish striped mullet and forkbeard, resulted in a weekly intake slightly below the established provisional tolerable weekly intake.

Heavy metals in the aquatic environment of the Southern Adriatic Sea, Italy: macroalgae, sediments and benthic species.

Samples of sea urchins (Paracentrotus lividus), holothurians (Holothuria polii), green algae (Ulva lactuca. Codium vermilara and Enteromorpha prolifera) and sediments were collected from different coastal zones of the South Adriatic Sea (Italy). The occurrence of metals in macroalgae is poor especially if compared with that reported in other coastal areas affected by human activities, with the exception of Fe that showed high mean values (405 microg g(-1) dry wt.). Likewise, relationships between metal concentrations in holothurians and sediments were found, demonstrating that H. polii could serve as bioindicator for Hg and Cu.

Heavy metals in mussels (Mytilus galloprovincialis) from the lonian Sea, Italy.

Concentrations of six heavy metals (Hg, Pb, Cd, Cr, Zn, and Sn) were determined in mussels (Mytilus galloprovincialis) collected between June and September 1997 from 10 locations along a sound formed by two inlets (Mar Piccolo) near the Gulf of Taranto (Ionian Sea, Italy). The average concentrations of the heavy metals found in mussels samples were 0.15 mg/kg for Hg, 1.19 mg/kg for Pb, 0.64 mg/kg for Cd, 0.31 mg/kg for Cr, 5.15 mg/kg for Zn, and 0.54 mg/kg for Sn. The concentrations of heavy metals in mussels from the first inlet did not differ greatly from those observed in mussels from the second inlet. The concentrations of heavy metals in the mussels analyzed were below acceptable levels for human consumption.

Thyrotoxic periodic paralysis in a Caucasian man in treatment for Graves' disease.

Thyrotoxic periodic paralysis (TPP) is the main secondary form of hypokalemic periodic paralysis and is mostly associated with Graves' disease. Initially diagnosed in Asian countries, TPP has been sporadically reported in different populations of the Western World. Increased Na+/K(+)-ATPase activity seems to be responsible for the marked hypokalemia observed during the transient paralysis attacks. We report on a 35-year-old Italian man without history of hypokalemic periodic paralysis and hyperthyroidism, in treatment for Graves' disease, who suffered episodes of flaccid paralysis even with normal thyroid hormone levels. An insulin-glucose provocation test confirmed our diagnosis. Oral and parenteral potassium reverse the symptoms. Monitoring of thyroid function is also important to prevent further attacks.

[Intravenous amiodarone in the therapy of paroxysmal supraventricular tachycardias]. / L'amiodarone endovena nella terapia delle tachiaritmie parossistiche sopraventricolari.

The Authors evaluated the effectiveness and the tolerance of intravenous Amiodarone in 50 cases of recent onset paroxysmal supraventricular tachyarrhythmias. Fifty consecutive patients, aged 17 to 84 (mean 52 years), presenting with paroxysmal supraventricular tachycardia (PSVT, 33 cases) or atrial flutter (11 cases) or atrial fibrillation (6 cases), were given 300 mg of Amiodarone intravenously within 2 min, followed in 4 patients by 150 mg after 15 min. All patients were monitored for 1 hour; ECG and blood pressure were recorded at fixed times. Within 15 min sinus rhythm was restored in 88% of PSVT, in 27% of atrial flutter and in 17% of atrial fibrillation cases; the other cases of atrial flutter and fibrillation always showed a 48-81% reduction of the average heart rate within 15 min. We have evidenced neither significant modifications of blood pressure and ECG parameters (P-Q, QRS and Q-T duration) nor particular side effects, except for 2 cases in which brief hot flushes were reported. The Authors believe Amiodarone to be an effective and well tolerated drug for the above mentioned arrhythmias, particularly promptly acting in PSVT cases, in whom sinus rhythm was restored within 15 min in 88% and within 1 hour in 100% of the cases.