Canine babesiosis in Austria in the 21st century - A review of cases.

A retrospective study on 699 cases of canine babesiosis presented to veterinary clinics in eastern Austria were evaluated for the location where infection had presumably taken place. Of these, 542 (77.54%) had acquired the infection in Austria, while the majority of non-autochthonous cases came from neighboring countries, most notable Hungary. Both groups were recorded primarily in Vienna, eastern Lower Austria and Burgenland, but cases from the southern (Styria, Carinthia) and western (Upper Austria, Tyrol, Salzburg) provinces of the country were also recorded. Records were made all year round, with most cases in spring (46.6%) and fall (48.4%). The annual cases ranged from four to 58 (mean: 31.8) with large fluctuations and no visible trend for an in- or decrease. The tick vector of Babesia canis, Dermacentor reticulatus, is present in Austria but displays a very patchy distribution, and its occurrence and activity are not readily foretold, which might be a reason why its presumably increasing density in Europe is not reflected by increased incidences of canine babesiosis. Another factor that may influence the numbers of cases per year could be the application (or non-application) of acaricidal or repellent compounds. A limitation of this study is that bias is exerted by the location of the participating clinics, and by the unknown rate of infections that does not induce clinical symptoms and is likely not presented in veterinary practices and clinics. The data, however, clearly show that at least the lowlands of Austria are endemic for B. canis, and appropriate tick control must be advised all year round.

Co-infection with Babesia canis and Babesia gibsoni in a dog.

A four-year-old intact male Boxer, that had a history of travelling to Serbia, was referred for lethargy and anaemia. Shortly before the dog was referred, it was diagnosed twice with an infection with Babesia canis and was treated with imidocarb both times. A blood smear evaluation was indicative of the presence of intraerythrocytic piroplasms. After receiving inconclusive results regarding the type of piroplasm, the dog was diagnosed with simultaneous infections with B. canis and Babesia gibsoni via real-time polymerase chain reaction (rt-PCR) testing. The dog was treated with imidocarb, atovaquone and azithromycin, and in a follow-up examination, the PCR results were negative for B. canis and B. gibsoni. Several weeks later, the dog was presented again, and a PCR was positive for B. gibsoni. After atovaquone and azithromycin failed to eliminate the parasites, a therapy attempt using metronidazole, clindamycin and doxycycline was initiated. Six months after diagnosis, the treatment appeared successful in eliminating B. gibsoni. This case report describes the clinical findings of the co-infection and the initiated diagnostic and therapeutic approaches.

Complications and risk factors regarding the outcomes of canine babesiosis in Central Europe - A retrospective analysis of 240 cases.

The purpose of this study was to investigate retrospectively the prevalence of the complicated and uncomplicated forms of babesiosis and to evaluate various laboratory and clinical parameters of dogs infected with Babesia canis in order to assess their prognostic value regarding the outcomes of the disease. Medical records, complete blood count and serum biochemical analysis from the animal hospital information system of 240 dogs were reviewed and evaluated retrospectively. Binary logistic regression analysis was used to ascertain correlations between alterations in the obtained parameters and survival probability. The results showed that creatinine levels of more than 5 mg/dL and phosphate levels of more than 3 mmol/L have a highly significant link to death (P ≤ 0.001). Albumin levels of <2.2 g/dL (P = 0.003) and a rectal body temperature below 38 °C (P ≤ 0.001) may also serve as prognostic markers for the severity of the disease. If renal involvement was present, 33.9% of the dogs died, while 40.0% of the dogs died in the presence of pancreatitis. The parameters creatinine, phosphate, albumin and rectal temperature serve as reliable predictive markers of an increased risk of death in the case of an infection with B. canis.