Sublethal behavioral impacts of resource limitation and insecticide exposure reinforce negative fitness outcomes for a solitary bee.

Contemporary landscapes present numerous challenges for bees and other beneficial insects that play critical functional roles in natural ecosystems and agriculture. Pesticides and the loss of food resources from flowering plants are two stressors known to act together to impair bee fitness. The impact of these stressors on key behaviors like foraging and nesting can limit pollination services and population persistence, making it critical to understand these sublethal effects. We investigated the effects of insecticide exposure and floral resource limitation on the foraging and nesting behavior of the solitary blue orchard bee, Osmia lignaria. Bees in field cages foraged on wildflowers at high or low densities, some treated with the common insecticide, imidacloprid, in a fully crossed design. Both stressors influenced behavior, but they had differential impacts. Bees with limited food resources made fewer, but longer foraging trips and misidentified their nests more often. Insecticide exposure reduced bee foraging activity. Additionally, insecticides interacted with bee age to influence antagonistic behavior among neighboring females, such that insecticide-exposed bees were less antagonistic with age. Our findings point towards mechanisms underlying effects on populations and ecosystem function and reinforce the importance of studying multiple drivers to understand the consequences of anthropogenic change.

Past insecticide exposure reduces bee reproduction and population growth rate.

Pesticides are linked to global insect declines, with impacts on biodiversity and essential ecosystem services. In addition to well-documented direct impacts of pesticides at the current stage or time, potential delayed "carryover" effects from past exposure at a different life stage may augment impacts on individuals and populations. We investigated the effects of current exposure and the carryover effects of past insecticide exposure on the individual vital rates and population growth of the solitary bee, Osmia lignaria Bees in flight cages freely foraged on wildflowers, some treated with the common insecticide, imidacloprid, in a fully crossed design over 2 y, with insecticide exposure or no exposure in each year. Insecticide exposure directly to foraging adults and via carryover effects from past exposure reduced reproduction. Repeated exposure across 2 y additively impaired individual performance, leading to a nearly fourfold reduction in bee population growth. Exposure to even a single insecticide application can have persistent effects on vital rates and can reduce population growth for multiple generations. Carryover effects had profound implications for population persistence and must be considered in risk assessment, conservation, and management decisions for pollinators to mitigate the effects of insecticide exposure.

A meta-analysis of single visit pollination effectiveness comparing honeybees and other floral visitors.

PREMISE: Many animals provide ecosystem services in the form of pollination including honeybees, which have become globally dominant floral visitors. A rich literature documents considerable variation in single visit pollination effectiveness, but this literature has yet to be extensively synthesized to address whether honeybees are effective pollinators. METHODS: We conducted a hierarchical meta-analysis of 168 studies and extracted 1564 single visit effectiveness (SVE) measures for 240 plant species. We paired SVE data with visitation frequency data for 69 of these studies. We used these data to ask three questions: (1) Do honeybees (Apis mellifera) and other floral visitors differ in their SVE? (2) To what extent do plant and pollinator attributes predict differences in SVE between honeybees and other visitors? (3) Is there a correlation between visitation frequency and SVE? RESULTS: Honeybees were significantly less effective than the most effective non-honeybee pollinators but were as effective as the average pollinator. The type of pollinator moderated these effects. Honeybees were less effective compared to the most effective and average bird and bee pollinators but were as effective as other taxa. Visitation frequency and SVE were positively correlated, but this trend was largely driven by data from communities where honeybees were absent. CONCLUSIONS: Although high visitation frequencies make honeybees important pollinators, they were less effective than the average bee and rarely the most effective pollinator of the plants they visit. As such, honeybees may be imperfect substitutes for the loss of wild pollinators, and safeguarding pollination will benefit from conservation of non-honeybee taxa.

Pesticide and resource stressors additively impair wild bee reproduction.

Bees and other beneficial insects experience multiple stressors within agricultural landscapes that act together to impact their health and diminish their ability to deliver the ecosystem services on which human food supplies depend. Disentangling the effects of coupled stressors is a primary challenge for understanding how to promote their populations and ensure robust pollination and other ecosystem services. We used a crossed design to quantify the individual and combined effects of food resource limitation and pesticide exposure on the survival, nesting, and reproduction of the blue orchard bee Osmia lignaria. Nesting females in large flight cages accessed wildflowers at high or low densities, treated with or without the common insecticide, imidacloprid. Pesticides and resource limitation acted additively to dramatically reduce reproduction in free-flying bees. Our results emphasize the importance of considering multiple drivers to inform population persistence, management, and risk assessment for the long-term sustainability of food production and natural ecosystems.