RESUMO
Uniaxial hyperbolic materials enable excitation of phonon polaritons with utrahigh wavevectors that have been shown to be promising for many optical and thermal radiative applications and thus have attracted much attention recently. However, the characteristics of surface and volume phonon polaritons excited with uniaxial hyperbolic materials that exhibit in-plane anisotropy or in-plane isotropy have not been discussed thoroughly and some issues have so far remained elusive. In this paper, we conducted a comprehensive investigation on surface and volume phonon polaritons in a bulk or a thin slab of hexagonal boron nitride (hBN). We clarified the excitation, characteristics and topology of surface and volume phonon polaritons in such a uniaxial hyperbolic material. In particular, we showed that hyperbolic surface phonon polaritons (HSPhPs) can exist in the Type I hyperbolic band of hBN with confined wavevectors when the optic axis (OA) is parallel to the surface. For a thin hBN slab, we revealed a split of HSPhPs and a smooth transition between HSPhPs and HVPhPs in the Type II hyperbolic band. Furthermore, we also identified non-Dyakonov surface phonon polaritons excited without evanescent ordinary waves. These findings may extend the understanding of phonon polaritons in hyperbolic materials and offer new theoretical guidance for the design of infrared optical devices with hyperbolic materials.
RESUMO
OBJECTIVE: To investigate the effect of recombinant adenovirus-mediated bone morphogenetic protein 9 (BMP-9) and erythropoietin (EPO) genes co-transfection on osteogenic differentiation of adipose-derived stem cells (ADSCs) in vitro. METHODS: The inguinal adipose tissue was harvested from 4-month-old New Zealand rabbits, ADSCs were isolated with enzyme digestion and adherence method, and multipotent differentiation capacity was identified. The 3rd generation ADSCs were divided into 5 groups: normal cells (group A), empty plasmid control group (group B), BMP-9 or EPO recombinant adenovirus transfected cells (groups C and D), BMP-9 and EPO recombinant adenovirus co-transfected cells (group E). The inverted phase contrast microscope was used to observe the cell growth at 7 days; the expression of cell fluorescence was observed under a fluorescence microscope at 14 days, and viral transfection efficiency was calculated at 48 hours; Western blot was used to detect the expressions of BMP-9 and EPO proteins at 14 days. The expression of alkaline phosphatase (ALP) activity was detected at 3, 7, and 14 days after osteogenic induction, and alizarin red staining was used to detect calcium nodules formation and real-time fluorescence quantitative PCR to detect the expressions of osteopontin (OPN) and osteocalcin (OCN) at 3 weeks. RESULTS: At 7 days after transfected, some cells showed oval, round, and irregular shape under the inverted phase contrast microscope in groups A and B; a few fusiform cells were observed in groups C and D; oval cells increased obviously, and there were only few round cells in group E. The fluorescence microscope observation showed that BMP-9 and EPO, BMP-9/EPO recombinant adenovirus could stably transfected ADSCs, with transfection efficiency of 80%-93%. The expressions of BMP-9 and EPO proteins significantly higher in group E than the other groups by Western blot (P < 0.05). The ALP activity significantly increased in group E when compared with that in the other groups at 3, 7, and 14 days after osteogenic induction (P < 0.05); the number of calcium nodules in group E was significantly more than that in the other groups (P < 0.05). Real-time fluorescence quantitative PCR showed that OPN and OCN genes expressions were significantly higher in group E than other groups (P < 0.05), and in groups C and D than groups A and B (P < 0.05). CONCLUSION: Recombinant adenovirus-mediated BMP-9 and EPO genes can transfect ADSCs, which can stably express in ADSCs, BMP-9/EPO genes co-transfection can more promote the expressions of osteoblast-related genes and protein than non-transfected and single gene transfection.