[Effects of salinomycin on proliferative, migratory and invasive property of tongue squamous cell carcinoma cells in vitro].

PURPOSE: To investigate the effects of salinomycin on proliferative, migratory and invasive properties of tongue squamous cell carcinoma cells, and to explore its possible mechanism. METHODS: CCK8 assay was used to detect the effect of salicamycin and cisplatin on proliferative abilities of CAL-27 cells and EA.hy926 cells. The invasive and migratory ability was detected by Transwell assay. The protein expressions of E-cadherin, vimentin and ß-catenin was evaluated by Western blot. SPSS20.0 software package was used to analyze the data. RESULTS: Salicamycin can effectively inhibit proliferation of CAL-27 cells, and the inhibitive ability of salicamycin on the proliferation was stronger than that of cisplatin. CAL-27 cells were treated by salinomycin (4 µmol/L) before invasive and migratory abilities were examined. Compared with control group, the number of invasive and migratory cells in the salinomycin-treated group was significantly decreased (P＜0.05). Western blot analysis showed that the protein expression of vimentin and ß-catenin was significantly down-regulated. The expression of E-cadherin was significantly increased with the increase of salicamycin concentration. CONCLUSIONS: The proliferative, invasive and migratory ability of CAL-27 cells can be inhibited by salinomycin, which may be related to the inhibition of epithelial-mesenchymal transitions.

[In vivo study of 3D printed porous tantalum implant on osseointegration].

OBJECTIVE: This work aims to investigate the effect of porous tantalum and porous titanium on osseointegration. METHODS: Two kinds of porous materials with same microporous parameters, namely, porous tantalum and porous titanium, were fabricated by computer-aided design (CAD) modeling and 3D printing technology. A defect model was established in 24 New Zealand white rabbits in the bilateral femoral lateral malleolus at the left and right side of each animal. Then, animals were randomly divided into two groups, and bone defects were repaired by porous tantalum and porous titanium (experimental and control groups, respectively). Animals were sacrificed at two, four, and eight weeks after implantation. Gross observation and methylene blue-acid fuchsin staining were used to observe osseointegration of the implant and bone interface, and the osseointegration strength of implant bone interface was tested by push-out test. RESULTS: At two, four, and eight weeks after operation, the new bone tissue in the two groups increased gradually, and new bone trabecula appeared and grew into the pores of the materials. No significant difference (P>0.05) in osteogenesis and the strength of implant bone tissue interface between the two groups was observed. CONCLUSIONS: 3D
printed porous tantalum implants, which exhibit comparable osseointegration capabilities to porous titanium implants, can form an early biological combination with bone tissue.