RESUMO
Collagen membranes are widely used in guided bone regeneration (GBR) because of their good biocompatibility and low immunogenicity. As a bioderived collagen membrane, small intestinal submucosa (SIS) has good regenerative potential for soft tissue repair, but it lacks sufficient mechanical properties for GBR application unless properly modifided. Epigallocatechin-3-gallate (EGCG) is a natural cross-linking agent featuring osteoinductive activity. In this study, we modified SIS by EGCG cross-linking, and such modified materials were characterized both in vitro and in vivo. The results showed that EGCG cross-linking significantly improved the mechanical properties and hydrophilicity of SIS while maintaing good cytocompatibility. Compared to SIS, EGCG-cross-linked SIS (E-SIS) enhanced the adhesion of fibroblasts and preosteoblasts and promoted the osteogenic differentiation of MC3T3-E1 cells cultured on the materials. In a rat cranial defect model, E-SIS material showed better occlusion effect than SIS material. Most importantly, E-SIS material accelerated bone regeneration more than SIS material and even a commercially available GBR membrane. Taken together, we conclude that E-SIS is a promising material as a GBR membrane.
RESUMO
OBJECTIVE: To determine the current status and influence factors of reporting quality of the Chinese meta-analysis on stomatology. METHODS: A comprehensive electronic search was carried out through Chinese BioMedical Literature Database (CBM), VIP Database for Chinese Technical Periodicals (VIP) and China National Knowledge Infrastructure (CNKI) and a hand searching was also performed through 19 stomatological journals in Chinese to identify meta-analysis on stomatology. Two reviewers took responsibility for study inclusion, data extraction and reporting quality assessment with preferred reporting items for systematic reviews and meta-analysis (PRISMA) in duplicate and any disagreement was resolved by discussion. RESULTS: A total of 34 meta-analysis on stomatology were eligible, most of them were on oral medicine and oral and maxillofacial surgery, and mainly focusing on etiology, prevention and treatment of oral diseases. The number of the meta-analysis increased during recent years. Reporting quality of the meta-analysis was not high and the PRISMA scored (13.6 ± 4.2). The main factors that influenced the reporting quality of meta-analysis were published on evidence-based medicine journals (adjusted ß = 0.53, t = 4.15, P < 0.001) and year of publication (adjusted ß = -0.44, t = -3.28, P = 0.001). The sensitivity analysis showed that this outcome was stable. CONCLUSIONS: Reporting quality of the Chinese meta-analysis on stomatology is low. To provide sufficient evidence to the clinicians and promote the development of evidence-based dentistry in China, experts on stomatology should study the knowledge of evidence-based medicine and comply with PRISMA statement when producing the meta-analysis.
