Influence of nitrogen-based fertilization on nitrates occurrence in groundwater of hilly vineyards.

Nitrogen losses from intensive agricultural production may end up as high nitrate (NO3-) concentrations in groundwater, with a long-term impact on groundwater quality. The main objective of this study was to evaluate the impact of fertilization practices used for grape cultivation on groundwater quality of Tidone Valley, northwest of Italy, following an integrated socio-hydrogeological approach that consists on (i) the involvement of 175 farmers in the description of agricultural and fertilization practices, using a survey of ad hoc questionnaires, (ii) the evaluation of NO3- occurrence in groundwater and (iii) the identification of NO3- sources through isotopic and hydrochemical analysis. In this area, as for certain particular Apennines shallow aquifers, groundwater is of reduced interest due to its limited storage capacity and there are insufficient wells currently monitored by the local Environmental Agency (ARPAE) to evaluate the impact of agricultural fertilization on existing local aquifers. Farmers' questionnaires results highlighted an extensive use of inorganic nitrogen fertilization and a tendency of farmers to follow their own experience for fertilization. Chemical analyses revealed high variability of major and trace elements concentrations isotope data. NO3- concentrations were significantly higher in deeper wells with respects to shallow wells. Isotopic results indicated that groundwater NO3- origin is inorganic, in agreement with the land use and the declared viticultural practices. Comparing groundwater NO3- occurrence from the studied area with values of entire Emilia-Romagna Region, only 7.7% of groundwater samples showed values above the EQS. (50 mg NO3-/L) between Nov 2017 and Sept 2018, while in the entire region 11.5% of groundwater samples showed values above the EQS in the same period. Considering that the vineyards surface in the studied area represents almost 75% of the entire regional vineyard surface, the obtained results suggest a low to moderate impact of viticulture on NO3-concentration of regional groundwater.

The WaterProtect governance guide: Experiences from seven agricultural and drinking water production catchments across Europe.

Although collaborative, multi-level and adaptive governance is put forward as the right approach to manage water resources, it remains a challenge for local water managers to engage with multiple understandings and perspectives and to move towards more transdisciplinary approaches in water governance. In this research, we address this need by proposing a practical guide for water governance assessment and improvement, existing of three successive steps, i.e. (1) an assessment of water governance, (2) the implementation of a multi-actor process and (3) an evaluation of achievements. This guide is tested in the seven action labs of the WaterProtect project, which have in common that they are drinking water catchments suffering from agricultural pollution throughout Europe. By implementing the guide in the different action labs, we find that water managers appreciated the integrated approach of the guide, helping them in the efficient organization of the water governance process while taking into account the human dimension. Furthermore action lab leaders evaluated the governance guide as being hands-on and practical, allowing them to redirect and open up the governance process. Despite this, they welcomed the presence of a facilitator, who boosted the local action lab leaders' confidence and motivation to put things into motion. Looking at the results, we find that over the different action labs a lot of progress has been made towards intermediary goals, however that an improvement of the water quality cannot be expected in the short time frame of a project. We therefore recommend improving the design of the guide with insights from transition management, which will allow local water managers to better address change at different time-scale levels.

First evaluation of pesticides occurrence in groundwater of Tidone Valley, an area with intensive viticulture.

Agricultural practice often involves an intensive and incorrect use of pesticides and fertilizers. These chemicals can leach through the soil profile and contaminate groundwater, including drinking water. For this reason, an effective groundwater monitoring is strongly advisable. The aim of this study was to investigate the groundwater contamination by plant protection products (PPPs) on a hilly area situated in the Tidone Valley, North-West of Italy, a region characterized by an intensive viticulture production. This area is not included in the national groundwater monitoring plan and therefore scarce information is available regarding the quality of groundwater, even though the local Environmental Agency previously revealed the occurrence of PPPs at values higher than the Environmental Quality Standard downstream this area. Hence, a monitoring wells network was developed following an upstream-downstream criterion, a list of pesticides to be monitored, based on a multi-actor approach, and an analytical method for PPPs detection and quantification. The analytical approach involved solid phase extraction followed by High-Performance Liquid Chromatography tandem mass spectrometry. The results of three monitoring campaigns revealed the occurrence of seven PPPs at a level higher than EQS for groundwater (0.1 µg/L) in 30% of the wells. The main pesticides detected were Chlorantraniliprole, Dimethomorph, Fluopicolide, Metalaxyl-M, Penconazole, and Tetraconazole, all commonly used in viticulture, together with S-metolachlor, authorized for cereal cropping. Statistical analysis revealed a significant influence of the sampling time, slope of the soil surrounding the wells, wells depth and wells location on the concentration of five PPPs. Therefore, the results obtained show that the improper use of PPPs for grapevine cultivation may cause groundwater contamination and suggest the need for a deeper analysis of territorial reality, including hydrology studies and farmer behavior and for an urgent introduction of best management practices and mitigation measures to promote a sustainable use of PPPs in viticulture.

Ecological and human exposure assessment to PBDEs in Adige River.

The interest for environmental issues and the concern resulting from the potential exposure to contaminants were the starting point to develop methodologies in order to evaluate the consequences that those might have over both the environment and human health. Considering the feature of POPs, including PBDEs, such as bioaccumulation, biomagnification, long-range transport and adverse effects even long time after exposure, risk assessment of POPs requires specific approaches and tools. In this particular context, the MERLIN-Expo tool was used to assess the aquatic environmental exposure of Adige River to PBDEs and the accumulation of PBDEs in humans through the consumption of possible contaminated local aquatic food. The aquatic food web models provided as output of the deterministic simulation the time trend of concentrations for twenty years of BDE-47 and total PBDEs, expressed using the physico-chemical properties of BDE-47, in aquatic organisms of the food web of Adige River. For BDE-47, the highest accumulated concentrations were detected for two benthic species: Thymallus thymallus and Squalius cephalus whereas the lowest concentrations were obtained for the pelagic specie Salmo trutta marmoratus. The trend obtained for the total PBDEs, calculated using the physico-chemical properties of BDE-47, follows the one of BDE-47. For human exposure, different BDE-47 and total PBDEs concentration trends between children, adolescent, adults and elderly were observed, probably correlated with the human intake of fish products in the daily diet and the ability to metabolize these contaminants. In detail, for the adolescents, adults and elderly a continuous accumulation of the target contaminants during the simulation's years was observed, whereas for children a plateau at the end of the simulation period was perceived.

Combined ecotoxicological risk assessment in the frame of European authorization of pesticides.

Organisms are frequently exposed to mixtures of chemical contaminants in the environment, causing a potential "cocktail effect", or combined effect. The joint action of different molecules with similar or different modes of action could result in a potentially unlimited number of additives, synergistic or antagonistic combinations. Since the large number of contaminants makes it impossible to perform ecotoxicity tests for each potential mixture, a robust approach for prospective environmental risk assessment of chemical mixtures is needed. A number of recent publications by the European Commission and the authorities in charge prove the increasing interest that is spreading in the European community towards the topic of the assessment of chemical mixtures. The current EU regulation for Plant Protection Products authorization (Reg. 1107/2009 EC) explicitly requires the evaluation of the potential combined effects of active substances. We reviewed current methods and limitations of mixture assessment of pesticides (7 fungicides and 4 herbicides) through the analysis of the approaches adopted to investigate possible risks for different non-target organisms. The Concentration Addition (CA) approach was the most used approach to predict multiple toxicity to non-target organisms. The guidance for birds and mammals first introduced standard procedures to assess the multiple toxicity based on on CA concept. The recent aquatic EFSA guidance introduced some requirements to evaluate potential mixture toxicity, while the current guidance requirements for terrestrial organisms still lack clear indications on how to conduct the assessment. Moreover, new indications come from the draft guidance for the assessment of terrestrial plants and in-soil organisms. However, the approval and implementation of these new guidelines are still at a developmental stage. Some final considerations are drawn on the future possibilities to improve risk assessment procedures so as to identify harmful effects of pesticides mixtures on non-target organisms.

Ecological status and sources of anthropogenic contaminants in mangroves of the Wouri River Estuary (Cameroon).

Mangroves are critically threatened by human activities, despite the important ecosystem functions and services they provide. Mangroves in Cameroon represent no exception to the worldwide trend of mangrove destruction, especially around Douala, on the Wouri river estuary. In two sites around Douala, we assessed the presence of sterols, PAHs, PCBs, DEHP, DDT and its metabolite p,p'-DDE and potentially toxic metals in sediment samples. As a proxy of ecological quality, we measured the diversity and abundance of macrobenthos assemblages. We detected p,p'-DDE contamination, with concentrations higher than 3µgkg(-1) in 16 out of 26 samples which were attributed to recent widespread use of DDT. The detection of sterols revealed faecal contamination. Significant sensitivity of the macrobenthos to contaminants was revealed, with possible implications on the overall mangrove vulnerability to climate change and on the provision of ecosystem services to local populations.

A new approach to assessing the water footprint of wine: an Italian case study.

Agriculture is the largest freshwater consumer, accounting for 70% of the world's water withdrawal. Water footprints (WFs) are being increasingly used to indicate the impacts of water use by production systems. A new methodology to assess WF of wine was developed in the framework of the V.I.V.A. project (Valutazione Impatto Viticoltura sull'Ambiente), launched by the Italian Ministry for the Environment in 2011 to improve the Italian wine sector's sustainability. The new methodology has been developed that enables different vines from the same winery to be compared. This was achieved by calculating the gray water footprint, following Tier III approach proposed by Hoekstra et al. (2011). The impact of water use during the life cycle of grape-wine production was assessed for six different wines from the same winery in Sicily, Italy using both the newly developed methodology (V.I.V.A.) and the classical methodology proposed by the Water Footprint Network (WFN). In all cases green water was the largest contributor to WF, but the new methodology also detected differences between vines of the same winery. Furthermore, V.I.V.A. methodology assesses water body contamination by pesticides application whereas the WFN methodology considers just fertilization. This fact ended highlights the highest WF of vineyard 4 calculated by V.I.V.A. if compared with the WF calculated with WFN methodology. Comparing the WF of wine produced with grapes from the six different wines, the factors most greatly influencing the results obtained in this study were: distance from the water body, fertilization rate, amount and eco-toxicological behavior of the active ingredients used.

Pesticides water decontamination in oxygen-limited conditions.

This study was undertaken to develop a laboratory bioreactor, with a functioning principle similar with that of biobed systems but working in oxygen-limited conditions, suitable for decontaminating wastewater mixtures with pesticides. The system is composed by two cylindrical plastic containers. The first one, where the pesticides solution is collected, is open, whereas the second one, where the biomass is disposed, is closed. The pesticides solution was pumped at the biomass surface and subsequently recollected and disposed in the first container. Four pesticides with different physical-chemical characteristics were tested. The results obtained showed a relatively good capacity of the developed prototype to decontaminate waste water containing the mixture of pesticides. The time of the experiment, the number of cycles that the solution made in the system and the environmental temperature have a significantly influence for the decontamination of acetochlor and chlorpyrifos whereas for the decontamination of terbuthylazine and metalaxyl no significant influence was observed. Even if the present prototype could represent a valid solution to manage the water pesticides residues in a farm and to increase the confidence of bystanders and residents, the practical difficulties when replacing the biomass could represent a limit of the system.