RESUMO
Comparative analysis was performed with a CFo ATP synthase subunit II homologue (CFo-II) derived from marine or fresh-water algae. The marine algae-derived CFo-II-transformed Escherichia coli grew and accumulated ATP more vigorously in NaCl or Cadmium containing medium, suggesting that this gene was useful for the development of stress-tolerant plant.
Assuntos
Trifosfato de Adenosina/metabolismo , Escherichia coli/metabolismo , Eucariotos/enzimologia , Água Doce/microbiologia , ATPases Translocadoras de Prótons/química , ATPases Translocadoras de Prótons/metabolismo , Água do Mar/microbiologia , Proliferação de Células , Clonagem Molecular , Escherichia coli/genética , Subunidades Proteicas , Proteínas Recombinantes/metabolismo , Homologia de Sequência de Aminoácidos , Especificidade da EspécieRESUMO
Here we report the isolation and characterization of the Chlamydomonas sp. W80-derived CFo ATP synthase subunit II WCFII homologue gene. Intracellular ATP concentration and stress-tolerance of Escherichia coli were increased by transformation with WCFII. We propose that WCFII-mediated stress tolerance is due to enhanced accumulation of intracellular ATP.
Assuntos
Complexos de ATP Sintetase/genética , Chlamydomonas/genética , Sequência de Aminoácidos , Animais , Chlamydomonas/efeitos dos fármacos , Dados de Sequência Molecular , Estresse Oxidativo , Paraquat/farmacologia , Subunidades Proteicas/genética , Proteínas de Protozoários/genética , Alinhamento de Sequência , Homologia de Sequência de AminoácidosRESUMO
A novel gene with antistress activities against both salt (NaCl) and cadmium stresses was isolated from the cDNA library of halotolerant green alga Chlamydomonas sp. strain W80 by a functional expression screening with Escherichia coli. The C-terminal region of this protein is responsible for the antistress activity, because N-terminal truncated clone of this gene retains the antistress activity, and the C-terminal truncated clone loses the activity. In the C-terminal region, there is a histidine and aspartic acid-rich domain (HD-rich domain).
Assuntos
Adaptação Fisiológica/genética , Chlamydomonas/genética , Chlamydomonas/fisiologia , Resistência a Medicamentos/genética , Sequência de Aminoácidos , Animais , Cádmio/metabolismo , Cádmio/farmacologia , Chlamydomonas/efeitos dos fármacos , DNA de Algas/química , DNA de Algas/genética , DNA de Protozoário/química , DNA de Protozoário/genética , Escherichia coli/genética , Viabilidade Microbiana , Dados de Sequência Molecular , Análise de Sequência de DNA , Deleção de Sequência , Cloreto de Sódio/farmacologia , Microbiologia da ÁguaRESUMO
The medical applications of photosynthetic bacteria are summarized. Photosynthetic bacteria can produce various types of physiological active substance such as vitamin B(12), ubiquinone (coenzyme Q10), 5-aminolevulinic acid (ALA), porphyrins and RNA. In particular, photosynthetic bacterial ALA was commercially applied to cancer diagnosis and treatment. Recently, ALA has been applied to the treatment of acne vulgaris and the suppression of the inflammatory response to coronary and iliac injuries. In addition, the recent applications of RNA from a marine photosynthetic bacterium as a medical supplement for immune improvement and suppression of infection are described. Furthermore, the feasible application of a biopolymer consisting of RNA from a photosynthetic bacterium as a drug delivery system (DDS) to cancer treatment is described.