RESUMO
Indonesia first reported SARS-CoV-2 in March 2020. From March to April, clinical microbiology laboratory Universitas Indonesia in Jakarta received 4617 specimens with 12.6% positivity rate and 22% asymptomatic case. The result of this study could give an early picture of Indonesia's COVID 19 outbreak situation.
RESUMO
Pulsed-field gel electrophoresis (PFGE) revealed that multiple genetic variants of Salmonella typhi are simultaneously present in Southeast Asia and are associated with sporadic cases of typhoid fever and occasional outbreaks. Comparative analysis of PFGE patterns also suggested that considerable genetic diversity exists among S. typhi strains and that some PFGE patterns are shared between isolates obtained from Malaysia, Indonesia, and Thailand, implying movement of these strains within these regions of Southeast Asia, where they are endemic.
Assuntos
Eletroforese em Gel de Campo Pulsado/métodos , Salmonella typhi/genética , Salmonella typhi/isolamento & purificação , Sudeste Asiático/epidemiologia , Enzimas de Restrição do DNA , DNA Bacteriano/genética , DNA Bacteriano/isolamento & purificação , Variação Genética , Humanos , Epidemiologia Molecular , Febre Tifoide/epidemiologia , Febre Tifoide/microbiologiaRESUMO
Detection of Salmonella typhi in blood by culture of the mononuclear cell-platelet layer was compared with other methods currently used for the diagnosis of typhoid fever. Colonies of S. typhi were present in all mononuclear cell-platelet layer-positive cultures within 18 h of plating and were identified within an additional 10 min by a coagglutination technique. In contrast, identification of all positive cultures by conventional blood culture required 3 days.
Assuntos
Plaquetas/microbiologia , Leucócitos Mononucleares/microbiologia , Salmonella typhi/isolamento & purificação , Febre Tifoide/diagnóstico , Testes de Aglutinação , Humanos , Manejo de Espécimes , Fatores de TempoRESUMO
A DNA probe was used to detect Salmonella typhi from blood samples from 14 of 33 patients with culture-confirmed typhoid fever, using the equivalent of 2.5 ml of blood. In contrast, S. typhi was detected in 17 of the same 33 patients by culture of 8 ml of blood. The probe hybridized to blood samples of 4 of 47 patients from whom S. typhi was not isolated.