RESUMO
Exopolysaccharides (EPS) are produced by probiotic bacteria Lactiplantibacillus plantarum PRK7 and L. plantarum PRK11. The structure of EPS-7 and EPS-11 was characterized by Fourier-transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (NMR), scanning electron microscopy (SEM), gas chromatography-mass spectroscopy (GCMS), and thermogravimetric analysis (TGA). Further, in in vitro studies antioxidant, emulsion, and antibiofilm activity were investigated. The FTIR spectrum confirmed the presence of polysaccharides in EPS-7 and EPS-11, with absorbance at 1654.93 and 1655.33 cm-1, respectively. H1 NMR further confirmed the presence of glucose, galactose, xylose, and mannose. Sugar derivatives in EPS-7 and EPS-11 were further confirmed with GCMS. The SEM analysis revealed that EPS-7 had a weblike structure and EPS-11 had a smooth porous layer. The result of the TGA revealed that EPS-7 and EPS-11 had greater thermal stability at 319.1 and 300.1 °C, respectively. Furthermore, EPS-7 and EPS-11 showed a good percentage of free radical scavenging in DPPH (89.77 % and 93.1 %), ABTS (57.65 % and 58.63 %), hydroxyl radical scavenging (44.46 % and 40.308 %), and reducing power assay. The emulsion activity was confirmed with edible oils such as coconut oil, sesame oil, almond oil, castor oil, and neem oil. The highest emulsion activity for EPS-7 and EPS-11 was found with coconut and castor oil. In addition, the antibiofilm activity against pathogens revealed that EPS can prevent biofilm formation. Thus, it was found that EPS-7 and EPS-11 possess good structural characteristics and their biological activity makes them ideal for applications in the food and pharmaceutical industry.
