RESUMO
The motion of individual weaver ants from Oecophylla smaragdina was tracked within a small arena to study the algorithm that these ants use for navigation. The arena, a floor tile, was either clean or partly covered by a mild chemical repellent. Statistical properties of the observed motion of the ant can be described by a model that is analogous to the Langevin theory of the motion of Brownian particles. With each time step, the velocity of the ant changes by a random vector with a robust probability distribution. When the average ant encounters the chemical repellent it responds, like a particle seeing a potential energy barrier, by initially slowing before recovering towards its equilibrium state of motion. The model accounts for most qualitative properties of motion with a small number of parameters.
RESUMO
Escherichia coli single-stranded (ss)DNA binding (SSB) protein mediates genome maintenance processes by regulating access to ssDNA. This homotetrameric protein wraps ssDNA in multiple distinct binding modes that may be used selectively in different DNA processes, and whose detailed wrapping topologies remain speculative. Here, we used single-molecule force and fluorescence spectroscopy to investigate E. coli SSB binding to ssDNA. Stretching a single ssDNA-SSB complex reveals discrete states that correlate with known binding modes, the likely ssDNA conformations and diffusion dynamics in each, and the kinetic pathways by which the protein wraps ssDNA and is dissociated. The data allow us to construct an energy landscape for the ssDNA-SSB complex, revealing that unwrapping energy costs increase the more ssDNA is unraveled. Our findings provide insights into the mechanism by which proteins gain access to ssDNA bound by SSB, as demonstrated by experiments in which SSB is displaced by the E. coli recombinase RecA.
