Multisite-Sequential Cyclization To Construct 1,2,4-Triazole-Based N-Fused Heterocyclics.

A feasible protocol that uses atomic groups (KSCN, KSeCN, and NH2CN), o-bromobenzoyl hydrazides, and formyls as reaction factors to synthesize N-fused 1,2,4-triazole with benzothiazides, benzoselenazinones, and quinazolinones was proposed. The method overcomes the lengthy multistep synthesis, narrow substrate scope, and toxicity challenge induced by the use or production of hazardous substances. It also enables the development of fused-heterocyclic selenium and quinazolinone derivatives. Their fluorescent performance further demonstrates the practicability of this methodology.

Combining Lattice Boltzmann method and genetic algorithm to optimize the layout of artificial floating islands in river network in China.

How to solve the pollution problem of water environment in river network is a hot issue in the world. Artificial floating island is an efficient way to deal with water pollution. Taking Jiashan, Zhejiang, China, as an example, Lattice Boltzmann method (LBM) is used to simulate the law of hydrodynamics and pollutant transport in river network. Lattice Boltzmann equations are established in both artificial floating island and non-artificial floating island sections, and the river network boundary is automatically identified. The simulation results have high accuracy and are more suitable for the establishment of complex boundaries. On this basis, combined with the genetic algorithm (GA) module, the location of the artificial floating island is optimized, and the retention ratio of the pollution in optimized layout of artificial floating island is between and 2.4 and 7.2%. The research results of this paper can provide theoretical reference for the selection and location of artificial floating island in practical engineering.

Distribution, Phylogeny and Evolution of Clinical and Environmental Vibrio vulnificus Antibiotic-Resistant Genes.

Vibrio vulnificus is an emergent marine pathogen and is the cause of a deadly septicemia. However, the evolution mechanism of antibiotic-resistant genes (ARGs) is still unclear. Twenty-two high-quality complete genomes of V. vulnificus were obtained and grouped into 16 clinical isolates and 6 environmental isolates. Genomic annotations found 23 ARG orthologous genes, among which 14 ARGs were shared by V. vulnificus and other Vibrio members. Furthermore, those ARGs were located in their chromosomes, rather than in the plasmids. Phylogenomic reconstruction based on single-copy orthologous protein sequences and ARG protein sequences revealed that clinical and environmental V. vulnificus isolates were in a scattered distribution. The calculation of non-synonymous and synonymous substitutions indicated that most of ARGs evolved under purifying selection with the Ka/Ks ratios lower than one, while h-ns, rsmA, and soxR in several clinical isolates evolved under the positive selection with Ka/Ks ratios >1. Our result indicated that V. vulnificus antibiotic-resistant armory was not only confined to clinical isolates, but to environmental ones as well and clinical isolates inclined to accumulate beneficial non-synonymous substitutions that could be retained to improve competitiveness.

The bacterial community structure in epiphytic biofilm on submerged macrophyte Potamogetom crispus L. and its contribution to heavy metal accumulation in an urban industrial area in Hangzhou.

Submerged macrophytes and their epiphytic biofilms are important media for metal transport/transformation in aquatic environment. However, the bacterial community structure and the contribution of the epiphytic biofilm to the heavy metal accumulation remain unclear. Therefore, in this study, water, sediment, submerged macrophyte (Potamogeton crispus L.) and its epiphytic biofilm samples in three sites of the moat in the industrial area of Hangzhou were collected for analyzing. The bacterial community structure was significantly impacted by the TN concentrations, and Genus Aeromonas (24.5-41.8%), Acinetobacter (16.2-29.8%) and Pseudomonas (12.6-23.6%) dominated in all epiphytic biofilm samples, which had the heavy metal pollutant resistibility. The contents of Cr in biofilms (7.4-8.3 mg/kg, DW) were significantly higher than those in leaves (1.0-2.4 mg/kg, DW), while the contents of Cu (11.0-13.9 mg/kg, DW) in leaves were significantly higher than those in biofilms (0.7-3.9 mg/kg, DW) in all the three sites. The BCF values of metals in the biofilm were followed the order of YF < IC < ETS. The results indicated that the epiphytic biofilm had positive effects on the metal bioaccumulation, and the metal accumulation ability increased with the hydrodynamic forces. Bioaccumulation by the epiphytic biofilm may be an effective way for metal (especially Cr) remediation.

Air pollutant emissions from vehicles in China under various energy scenarios.

Estimations of air pollutant emissions from vehicles in China under different energy and emission abatement policy scenarios are presented in this paper. Three scenarios are designed: (i) "business as usual" (BAU); (ii) "advanced fuel economy" (AFE); and (iii) "alternative energy replacement" (AER). The CO, VOCs, NOx, PM10, and CO2 emissions are predicted to reach 105.8, 5.9, 7.5, 1.1, and 3522.6 million tons, respectively, in the BAU scenario by 2030. In the AFE scenario, the CO, VOCs, NOx, PM10, and CO2 emissions in 2030 will be abated by 23.8%, 18.6%, 25.3%, 18.2%, and 24.5% respectively compared with the BAU scenario. In the AER scenario, the CO and VOCs in 2030 will be further reduced by 15.9% and 6.1% respectively, while NOx, PM10, and CO2 will be increased by 10.7%, 33.3%, and 2.0% compared with AFE. In conclusion, our models indicate that the emission abatement policies introduced by governmental institutions are potentially viable, as long as they are effectively implemented.

[The dietary patterns and nutritional status of adult Yi people in Liangshan autonomous region].

This study was undertaken to know about the dietary pattern and nutrition status of adult Yi people in Liangshan autonomous region so that to provide evidence to ameliorate and promote the health and prevent nutrition-related diseases. 220 families, 468 inhabitants aged from 18 to 60, were sampled for the survey by a multistage, stratified, random, cluster process. Questionnaire-survey and measurement of height and weight were executed on the spot. Between rural and urban Yi inhabitants, there were differences in income, dietary pattern and eating habit. Compared with RNIs, dietary nutrients intakes of adult Yi people were irrational and differences lay between rural and urban. For urban inhabitants, the prevalence of overweight and obesity was 188%, and the prevalence of emaciation was 4.7%; while for rural inhabitants, the prevalence of emaciation was 9.8%, but no prevalence of overweight and obesity. There was obvious imbalance in energy and nutrients intakes of rural and urban adult Yi people in Liangshan. Emaciation, overweight and obesity prevalence was different between these two subgroups of population.