Bacterial specialists playing crucial roles in maintaining system stability and governing microbial diversity in bioremediation of oil-polluted sediments under typical deep-sea condition.

Ecologically, interactions and contributions of microbiota generalists and specialists remain largely unexplored in remediation of deep-sea oil pollution. Herein, ecological and evolutionary characteristics of the two taxa were comprehensively investigated in restoration of oil-polluted sediment at deep-sea microcosm. Niche-specialized taxa exhibited rapid speciation rate, more complex network structure and highly interspecific mutualism. In contrast, generalists possessed higher richness but with poor local performance, as evidenced by higher extinction rate, lower stability, and more interspecific antagonism. Generalists were the primary oil degraders, while specialists acted as auxiliaries promoting degradation via production of biofilm and biosurfactant. Evolutionarily, the continuous transition from specialists to generalists insured the exclusion of generalist at a relatively constant level for ecological trade-offs. Collectively, the findings emphasize the importance of specialists in facilitating oil degradation by elucidating their vital roles in maintaining system stability and regulating microbial diversity during process, and offer valuable guidance for designing remediation plans.

RNF144A-AS1 stabilizes TAF15 and promotes malignant biological behaviors of skin cutaneous melanoma.

LncRNAs have been demonstrated to regulate biological processes in malignant tumors. In our previous study, we identified the immune-related LncRNA RNF144A-AS1 as a potential regulator in SKCM. However, its precise function and regulatory mechanism remain unclear. In this study, we observed upregulation of RNF144A-AS1 in SKCM and found that knockdown of RNF144A-AS1 suppressed proliferation, migration, invasion, and epithelial-mesenchymal transition abilities of melanoma cells. Mechanistically, as a high-risk prognostic factor, RNF144A-AS1 regulated biological processes of SKCM by interacting with TAF15 through an RNA-binding protein-dependent (RBP-dependent) manner. Furthermore, we confirmed that TAF15 activated downstream transcriptional regulation of YAP1 to modulate malignant behaviors in melanoma cells. In vivo experiments revealed that knockdown of RNF144A-AS1 inhibited tumorigenic capacity of melanoma cells and exhibited promising therapeutic effects. Collectively, these findings highlight the significance of the RNF144A-AS1/TAF15/YAP1 axis in promoting malignant behaviors in SKCM and provide novel insights into potential prognostic biomarkers and therapeutic targets for this disease.

Red and processed meat and pancreatic cancer risk: a meta-analysis.

Background: The relationship between red and processed meat consumption and pancreatic cancer risk is controversial and no study has looked specifically at the correlation for 6 years. We conducted a meta-analysis to summarize the evidence about the association between them. Methods: We systematically searched PubMed, Embase and Cochrane Library for studies of red or processed meat consumption and pancreatic cancer published from December 2016 to July 2022. We performed random-effects models to pool the relative risks from individual studies. Subgroup analyses were used to figure out heterogeneity. We also performed publication bias analysis. Results: Seven cohort studies and one case-control study that contained a total of 7,158 pancreatic cancer cases from 805,177 participants were eligible for inclusion. The combined RRs (95% CI) comparing highest and lowest categories were 1.07 (95% CI: 0.91-1.26; p = 0.064) for red meat and 1.04 (95% CI: 0.81-1.33; p = 0.006) for processed meat with statistically significant heterogeneity. Conclusion: This meta-analysis suggested that red and processed meat consumption has no relationship with pancreatic cancer risk.

Thermo-Responsive Polymer-siRNA Conjugates Enabling Artificial Control of Gene Silencing around Body Temperature.

PURPOSE: Controlling small interfering RNA (siRNA) activity by external stimuli is useful to exert a selective therapeutic effect at the target site. This study aims to develop a technology to control siRNA activity in a thermo-responsive manner, which can be utilized even at temperatures close to body temperature. METHODS: siRNA was conjugated with a thermo-responsive copolymer that was synthesized by copolymerization of N-isopropylacrylamide (NIPAAm) and hydrophilic N,N-dimethylacrylamide (DMAA) to permit thermally controlled interaction between siRNA and an intracellular gene silencing-related protein by utilizing the coil-to-globule phase transition of the copolymer. The composition of the copolymer was fine-tuned to obtain lower critical solution temperature (LCST) around body temperature, and the phase transition behavior was evaluated. The cellular uptake and gene silencing efficiency of the copolymer-siRNA conjugates were then investigated in cultured cells. RESULTS: The siRNA conjugated with the copolymer with LCST of 38.0°C exhibited ~ 11.5 nm of the hydrodynamic diameter at 37°C and ~ 9.8 nm of the diameter at 41°C, indicating the coil-globule transition above the LCST. In line with this LCST behavior, its cellular uptake and gene silencing efficiency were enhanced when the temperature was increased from 37°C to 41°C. CONCLUSION: By fine-tuning the LCST behavior of the copolymer that was conjugated with siRNA, siRNA activity could be controlled in a thermo-responsive manner around the body temperature. This technique may offer a promising approach to induce therapeutic effects of siRNA selectively in the target site even in the in vivo conditions.

Cumulative Ecological Risk and Problem Behaviors Among Adolescents in Secondary Vocational Schools: The Mediating Roles of Core Self-Evaluation and Basic Psychological Need Satisfaction.

Based on the Cumulative Risk Model, a single risk factor cannot play a decisive role, but the cumulative ecological risks may have complex superposition effects on adolescents' problem behaviors. However, although many studies have investigated the specific influences of single external risk factors on problem behaviors, the effect of cumulative ecological risk on problem behaviors and especially the underlying mechanisms therein have been under-investigated. Therefore, this study aimed to explore the effect of cumulative ecological risk on adolescents' problem behaviors, and the mediating effects of core self-evaluation and basic psychological needs satisfaction therein. To achieve this, 1,080 adolescents in secondary vocational schools were surveyed with the questionnaires of cumulative ecological risk, basic psychological need satisfaction, core self-evaluation, externalizing and internalizing problem behaviors. Results showed that: (a) cumulative ecological risk was positively related with both internalizing problem behavior and externalizing problem behavior; (b) core self-evaluation and basic psychological need satisfaction not only respectively but also sequentially mediated the relationship between cumulative ecological risk and two sorts of problem behaviors. These results provide some pivotal implications for the precaution and intervention of the adolescent problem behaviors.

Potential urinary monitoring of the enhanced permeability and retention effect using MMP-2-responsive poly(ethylene glycol) derivatives.

The enhanced permeability and retention (EPR) effect is fundamental to tumor-targeted drug delivery using nanoparticles. However, recent studies reported heterogeneity of the EPR effect, and companion diagnostics are considered to be key to predicting and optimizing the benefits of the EPR effect. Here, as a new material to simply endow the function of companion diagnostics to nanoparticles, we designed a poly(ethylene glycol) (PEG) derivative conjugated with low molecular fluorescent dye through synthetic substrate linker that can be cleaved in response to MMP-2, which is overexpressed in tumor extracellular matrix. Upon tumor accumulation, the low molecular fluorescent dye is released from the PEG and quickly excreted to urine, thereby reporting its tumor accumulation level as a fluorescent signal in the urine. In this study, this urinary reporter was conjugated with albumin, and the functionalized albumin exhibited efficient accumulation in various tumors. Importantly, the functionalized albumin exhibited significantly higher excretion of the fluorescent dye in the urine in mice with tumors compared with those without tumors. The PEG derivatives proposed in this study may be a promising tool to predict the EPR effect in individual cancer patients.

Voltage-gated sodium channels ß3 subunit promotes tumorigenesis in hepatocellular carcinoma by facilitating p53 degradation.

The voltage-gated sodium channels (VGSCs) are aberrantly expressed in a variety of tumors and play an important role in tumor growth and metastasis. Here, we show that VGSCs auxiliary ß3 subunit, encoded by the SCN3B gene, promotes proliferation and suppresses apoptosis in HepG2 cells by promoting p53 degradation. ß3 significantly increases HepG2 cell proliferation, promotes tumor growth in mouse xenograft models, and suppresses senescence and apoptosis. We found that ß3 knockdown stabilizes p53 protein, leading to potentiation of p53-induced cell cycle arrest, senescence, and apoptosis. Mechanistic studies revealed that ß3 could bind to p53, promoting p53 ubiquitination and degradation by stabilizing the p53/MDM2 complex. Our results suggest that ß3 is a novel negative regulator of p53 and a potential oncogenic factor.

Relations Between Dual Filial Piety and Life Satisfaction: The Mediating Roles of Individuating Autonomy and Relating Autonomy.

Filial piety (FP) was formerly a Confucian concept that specifies how children should treat their elders. In recent years, some psychologists have postulated that there are considerable overlaps between Chinese FP and notions found in other cultures. They have redefined FP as a contextualized personality emphasizing the psychological schema of parent-child interaction so that it fits universal cultural contexts. Based on this theory construction, this study aimed to examine the effects of reciprocal FP and authoritarian FP on life satisfaction and the mediating roles of individuating autonomy and relating autonomy therein. To do so, we recruited and surveyed 360 high school students in China. Subsequently, a mediation model based on the Dual Filial Piety Model and previous studies was tested. Results demonstrate that reciprocal FP predicted life satisfaction positively and that both individuating autonomy and relating autonomy played significant mediating roles in the relationship between reciprocal FP and life satisfaction. Moreover, authoritarian FP had a negative indirect effect on satisfaction through the mediating role of individuating autonomy, while authoritarian FP had a positive indirect influence on satisfaction through the mediating role of relating autonomy. The theoretical and practical implications of these findings are discussed.

Gratitude and Problem Behaviors in Adolescents: The Mediating Roles of Positive and Negative Coping Styles.

The current study investigated the relationship between gratitude, internalizing and externalizing problem behaviors, along with the mediating roles of positive and negative coping styles therein. A sample of 589 Chinese adolescents completed the Gratitude Questionnaire (GQ-6), the Simplified Coping Style Questionnaire (SCSQ), the Irritability, Depression, and Anxiety Scale (IDAS), and the Aggression Questionnaire (AQ). Results of structural equation modeling showed that (1) the total effects of gratitude on both internalizing and externalizing problem behaviors were all significant and (2) both positive and negative coping styles mediated the links between gratitude and two types of problem behaviors. Thus, cultivating gratitude and developing adaptive coping style may help adolescents rectify problem behaviors.