Analysis of Co-expression of HSP 70 and BCL 2 in Oral Precancer and Cancer Patients Using Immunohistochemistry.

Introduction: Understanding of molecular model of oral carcinogenesis has carried cancer chemotherapy far forward from conventional drug therapies. Small molecule inhibitors have gained acceptance as it has fewer adverse effects and also provide targeted drug therapy. The association of HSP 70 (Heat Shock Protein 70) and BCL 2 (B-cell lymphoma 2) proteins with oral precancer and cancer is already established. However, the complex interaction between these two proteins and how they affect each other's expression is still not understood completely. In our study, we aimed to correlate the expression of HSP 70 and BCL 2 with different histopathological grades of oral precancer and cancer tissue samples using tissue immunohistochemistry. Materials and Methods: Tissue samples were taken from a total of 250 patients (100 OPMDs and 150 OSCCs) and subjected to immunohistochemistry using anti-human mouse monoclonal antibodies to HSP70 and BCL2. Immunostaining was done, and the immunostaining intensity distribution (IID) index was calculated. Results and Discussion: Immunoreactivity scores for both HSP 70 and BCL 2 correlated with different grades of dysplasia. However, only HSP 70 had a statistically significant association (p = 0.066). We also found that HSP 70 showed an inverse correlation, with higher expression majorly seen in well-differentiated OSCCs. Conclusion: Our study unveiled the HSP 70-BCL 2 interaction and provides insights about how this might affect drug designing and help overcome therapeutic lags. However, further studies are needed to provide a comprehensive review of such interactions among various small molecules.

A Case Report with Current Updates and Immunohistochemistry Features of Intraosseous Schwannoma.

Schwannomas are benign peripheral nerve sheath neoplasms that grow slowly and arise from Schwann cells. Here we report a case of an intraosseous schwannoma, with recent updates of molecular characterization and immunohistochemical data, affecting the left mandibular ramus featuring multilocular well-defined radiolucency making it difficult to diagnose.

Huge complex composite odontoma of mandible.

A young female patient reported to the department of oral and maxillofacial surgery with complaint of asymptomatic swelling in left posterior region of lower jaw since 6 months. Complete intraoral and extraoral clinical examination was done. Routine radiographical investigations were advised. Based on clinical and radiographical findings she was provisionally diagnosed with odontoma of left mandible. It was a considerably giant mass with thinned out both cortical plates and the inferior border of the mandible. Although we assumed high risk of fracture of the mandible, successful surgical excision of the tumour was done using a minimally invasive intraoral approach and by sectioning the odontoma, sparing cortical bones. We managed to remove the whole of tumour without fracturing the mandible. Final histopathological report confirmed the initial diagnosis of complex composite odontoma. Patient is under regular follow-up.