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Tracking evolution of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within infected individuals will help elucidate coronavirus disease 2019 (COVID-19) pathogenesis and inform use of antiviral interventions. In this study, we developed an approach for sequencing the region encoding the SARS-CoV-2 virion surface proteins from large numbers of individual virus RNA genomes per sample. We applied this approach to the WA-1 reference clinical isolate of SARS-CoV-2 passaged in vitro and to upper respiratory samples from 7 study participants with COVID-19. SARS-CoV-2 genomes from cell culture were diverse, including 18 haplotypes with non-synonymous mutations clustered in the spike NH2-terminal domain (NTD) and furin cleavage site regions. By contrast, cross-sectional analysis of samples from participants with COVID-19 showed fewer virus variants, without structural clustering of mutations. However, longitudinal analysis in one individual revealed 4 virus haplotypes bearing 3 independent mutations in a spike NTD epitope targeted by autologous antibodies. These mutations arose coincident with a 6.2-fold rise in serum binding to spike and a transient increase in virus burden. We conclude that SARS-CoV-2 exhibits a capacity for rapid genetic adaptation that becomes detectable in vivo with the onset of humoral immunity, with the potential to contribute to delayed virologic clearance in the acute setting. Author SummaryMutant sequences of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) arising during any individual case of coronavirus disease 2019 (COVID-19) could theoretically enable the virus to evade immune responses or antiviral therapies that target the predominant infecting virus sequence. However, commonly used sequencing technologies are not optimally designed to detect variant virus sequences within each sample. To address this issue, we developed novel technology for sequencing large numbers of individual SARS-CoV-2 genomic RNA molecules across the region encoding the virus surface proteins. This technology revealed extensive genetic diversity in cultured viruses from a clinical isolate of SARS-CoV-2, but lower diversity in samples from 7 individuals with COVID-19. Importantly, concurrent analysis of paired serum samples in selected individuals revealed relatively low levels of antibody binding to the SARS-CoV-2 spike protein at the time of initial sequencing. With increased serum binding to spike protein, we detected multiple SARS-CoV-2 variants bearing independent mutations in a single epitope, as well as a transient increase in virus burden. These findings suggest that SARS-CoV-2 replication creates sufficient virus genetic diversity to allow immune-mediated selection of variants within the time frame of acute COVID-19. Large-scale studies of SARS-CoV-2 variation and specific immune responses will help define the contributions of intra-individual SARS-CoV-2 evolution to COVID-19 clinical outcomes and antiviral drug susceptibility.
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PURPOSE: The purpose of this study was to examine the relationship among emotional intelligence, empathy and facilitative communication ability of psychiatric nurses. METHODS: Participants were 173 conveniently selected psychiatric nurses from 10 hospitals in Korea. Data were collected from June to September, 2015 through self-reported questionnaires. RESULTS: Facilitative communication ability was significantly different depending on educational level (t=6.06, p=.003). There were significant positive correlations between emotional intelligence and facilitative communication ability (r=.61, p<.001), and also between empathy and facilitative communication ability (r=.63, p<.001). In the multiple regression, except for education level, emotional intelligence (β=.17, p=.003) and empathy (β=.21, p=.000) explained 38.2% of the variance in facilitative communication ability. CONCLUSION: These findings indicate that emotional intelligence and empathy are important factors in psychiatric nurses' facilitative communication ability. Accordingly, to improve the facilitative communication ability of psychiatric nurses, educational programs based on development of emotional intelligence and empathy are necessary.
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Comunicação , Educação , Inteligência Emocional , Empatia , Coreia (Geográfico)RESUMO
PURPOSE: The purpose of this study was to identify the relationship among the factors of self-awareness, other-awareness and communication ability, and how they relate to communication ability in nursing students. METHODS: For data collection 237 nursing students completed self-report questionnaires. Data were analyzed using independent t-test, one-way ANOVA, Pearson correlation coefficients, and stepwise multiple regression. RESULTS: Self-awareness, other-awareness and communication ability of the participants showed moderate levels of self-awareness, other-awareness, and communication ability. There were significant positive correlations between self-awareness and communication ability (r=.59, p<.001). and between other-awareness and communication ability (r=.22, p=.001). Social anxiety, private self-awareness, and internal other-awareness were significant factors, which explained about 37% of the variance in communication ability. CONCLUSION: These findings suggest that a systematic and effective curriculum focused on self-awareness and other-awareness should be developed for nursing students in order to promote their communication ability.
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Humanos , Ansiedade , Comunicação , Currículo , Coleta de Dados , Enfermagem , Estudantes de EnfermagemRESUMO
PURPOSE: This study was done to identify types and characteristics of gender role identity from the perspectives of nursing students who experience challenges and maladjustments that are distinct from students in other departments because nursing students have a curriculum that includes both academic courses and clinical practice. METHODS: Q Methodology was used to examine differences in the meaning of gender role identity among individual students. Data from 40 women nursing students, using 40 Q samples representing masculinity and femininity, were collected and analyzed. RESULTS: The participants' responses were classified into three types: 'Affectionate-sensitive type' represented by those who share traditional Korean feminine characteristics and are delicately sensitive. 'Affectionate-assertive type' represented by those who have strong characteristics of warm leaders, and are responsive and confident. 'Sensitive-expressive type' represented by those who are excellent in communication and have high empathic ability. CONCLUSION: Nursing professors or counselors need to provide nursing students with guidance and counseling related to adaptation to school life, career exploration, and career choice based on the their type of gender role identity.
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Feminino , Humanos , Escolha da Profissão , Aconselhamento , Currículo , Feminilidade , Identidade de Gênero , Masculinidade , Métodos , Enfermagem , Estudantes de EnfermagemRESUMO
PURPOSE: The purpose of this study was to identify the specific types and characteristics of gender role identity in view of male nursing students. METHOD: Q-methodology, a technique for extracting subjective opinions, was used. Forty participants completed the Q-sort activity, rating each statement relative to the others. The Q sample has two categories, representing masculinity and femininity, and each category has 20 statements, resulting in 40 adjectives. The collected data were analyzed by QUANL PC program. RESULTS: Three types of gender role identity of male nursing students were identified: 'Taciturn warmth type', 'assertive power type', 'empathic warmth type'. Despite the differences among the types in this research, male nursing students are likely to have the understanding and keep faith. CONCLUSION: Male nursing students were exposed to many problems because of the gendered culture of nursing education. To understand the male nursing students' gender role identity will help to promote adaptation in nursing field.
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Humanos , Masculino , Educação em Enfermagem , Feminilidade , Identidade de Gênero , Masculinidade , Enfermagem , Q-Sort , Estudantes de EnfermagemRESUMO
The purpose of this study is to analyze the school dietitian's cognition, practical use of environment-friendly agricultural products to provide fundamental information for the qualitative improvement in the school food service. The results of the study are as follows: 1. It was clearly recognized that the subsidy for the school food service is generally insufficient, the amount of additional appropriate subsidy for the school food service was different according to type of school and roughly 300~600 won in each of foods needs to be supported. 2. The environmentfriendly agricultural products that were often used in the school food service were vegetables and grains. 3. In the analysis of cognition and problems toward using environment-friendly agricultural products, the dietitians recognized that environment-friendly agricultural products should be appropriately used. A reason that environmentfriendly agricultural products were better than general agricultural products was composed of a use of less additive, good ingredient, and good outer condition. 4. In the analysis of countermeasure on increasing environment-friendly agricultural products in the school meal service, the response to that more additional subsidy was necessary for the school food service. The highest and the most positive effects after using environment-friendly agricultural products were students' health improvement and improvement in parents' satisfaction with the school food service.
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Humanos , Grão Comestível , Cognição , Serviços de Alimentação , Refeições , VerdurasRESUMO
PURPOSE: This descriptive study was conducted to identify the use of complementary and alternative therapies, and the perception and attitudes toward CAT in Korean adults. METHODS: The 1,010 study subjects were recruited from ten regions in South Korea. Data were collected from July to September, 2004. RESULTS: The 43.2% of the sample(n=436) had an experience of using CAT. The subjects using CAT was more likely to have high score in the CAT perception compared to the subjects with no experience of CAT(p=.001). The primary source of information about CAT was mass media(59.7%). The 52.4% of the sample answered that CAT was supplementary and the 27.7% answered CAT was applicable to chronic pain or musculoskeletal disorders. Subjects(34.8%) pointed out that the greatest problem was no scientific informant in the use of CAT. The primary reason of using CAT was to maintain their health or to prevent disease(44.0%), and the 59.0% of the subjects responded that CAT was effective. Majority of subjects were satisfied with CAT(63.3%) and experienced no side effects of CAT(90.6%). CONCLUSION: This study suggests that professional counseling and education for CAT is needed for Korean public as well as the research approach to test the effects of CAT.
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Adulto , Animais , Gatos , Humanos , Dor Crônica , Terapias Complementares , Aconselhamento , Educação , Coreia (Geográfico)RESUMO
PURPOSE: The purposes of this study were to measure the degree of uncertainty in illness, hope, and spiritual well-being and to examine the relationship among uncertainty in illness, hope, and spiritual well-being of cancer patients. METHOD: The data were derived from answers to questionnaires obtained from 93 cancer patients at one general hospital in J area from April 6, 2004 to May 8, 2004. The collected data were analyzed by SSPS 10.0. RESULTS: The mean score of uncertainty in illness of cancer patients was 55.96. The mean score of hope of cancer patients was 79.77, and spiritual well-being was 55.20. Uncertainty in illness of cancer patients was related negatively to hope(r=-.57, p=.000). Also uncertainty in illness of cancer patients was related negatively to spiritual well-being(r=-.57, p=.000). However, hope of cancer patients was related positively to spiritual well-being(r=.67, p=.000). CONCLUSION: Higher uncertainty in illness was related to lower hope and spiritual well-being of cancer patients. Based upon these results, nurses should help patients to improve their hope and spiritual well-being through mediation of uncertainty in illness.
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Humanos , Esperança , Hospitais Gerais , Negociação , Incerteza , Inquéritos e QuestionáriosRESUMO
The nature of the implant surface can directly influence cellular response, ultimately affecting the rate and quality of new bone tissue formation. The aim of this in vitro study was to investigate if human osteoblast-like cells, Saos-2, would respond differently when plated on disks of magnesium titanate and machined titanium. Magnesium titanate disks were prepared using Micro Arc Oxidation(MAO) methods. Control samples were machined commercially pure titanium disks. The cell adhesion, proliferation and differentiation were evaluated by measuring cell number, and alkaline phosphatase(ALPase) activity at 1 day and 6 day after plating on the titanium disks. Measurement of cell number and ALPase activity in Saos-2 cells at 1 day did not demonstrate any difference between machined titanium and magnesium titanate. When compared to machined titanium disks, the number of cells was reduced on the magnesium titanate disks at 6 day, while ALPase activity was more pronounced on the magnesium titanate. Enhanced differentiation of cells grown on magnesium titanate samples was indicated by decreased cell proliferation and increased ALPase activity.
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Humanos , Osso e Ossos , Adesão Celular , Contagem de Células , Proliferação de Células , Magnésio , Osteoblastos , Saturno , TitânioRESUMO
The success of an implant is determined by its integration into the tissue surrounding the biomaterial. Surface roughness is considered to influence the behavior of adherent cells. The aim of this in vitro study was to determine the effect of surface roughness on Saos-2 osteoblast-like cells. Titanium disks blasted with 75 micrometer aluminum oxide particles and machined titanium disks were prepared. Saos-2 were plated on the disks at a density of 50,000 cells per well in 48-well dishes. After 1 hour, 1 day, 6 days cell numbers were counted. One day, 6 days after plating, alkaline phosphatase(ALPase) activity was determined. Compared to experimental group, the number of cells was significantly higher on control group. The stimulatory effect of surface roughness on ALPase was more pronounced on the experimental group than on control group. These results demonstrate that surface roughness alters proliferation and differentiation of osteoblasts. The results also suggest that implant surface roughness may play a role in determining phenotypic expression of cells.
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Óxido de Alumínio , Contagem de Células , Osteoblastos , TitânioRESUMO
PURPOSE: The study was to identify the relationship between the spiritual well-being, family support and depression in cancer patients. METHOD: Data were collected by questionnaires from 116 inpatients with cancer at one university hospital in J area using Spiritual Well-being Scale, Family Support Scale, and BDI. The collected data were analyzed by SPSS WIN 12.0 program. RESULT: 1) The mean scores of well-being, family support, and depression were 107.28, 41.14, and 16.79 respectively. 2) There were significant differences in the spiritual well-being by age, education, religion, and social group. There were significant differences in the family support by age, education, and number of admission. There were significant differences in the depression by occupation and social group. 3) Depression was significantly correlated with spiritual well-being, and family support. 4) The most signifiant predictor which influenced depression in cancer patients was spiritual well-being, followed by occupation, age, family support. CONCLUSION: These results suggested that providing spiritual nursing intervention and enhancing family support will effectively decrease depression in cancer patients.
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Humanos , Depressão , Educação , Pacientes Internados , Enfermagem , Ocupações , Inquéritos e QuestionáriosRESUMO
PURPOSE: This study was done to identify the degree that perceived social support effects the self esteem of the elderly. METHOD: A structured questionnaire was administered to 203 elderly subjects from April 2003 to June 2003. The data were analyzed with an SPSS program for descriptive statistics, Pearson`s correlation coefficients, t-test, ANOVA, and stepwise multiple regression. RESULTS: The level of social support was moderate; family support received the highest score among sources of support. In types of support, appraisal support was the highest score and material was the lowest. The level of self esteem was also moderate. The score of social support showed a slightly high positive correlation with self esteem. In general characteristics, several variables were significantly related to self esteem. The most powerful predictor of self esteem was material support by family and the variance explained was 19.6%. A combination of material support by family, informational support by relatives, perceived economic status, perceived health status, and having a subject to depend on accounted for 39.8% of the variance in self esteem of the elderly. CONCLUSION: To increase the self esteem of the elderly, it is necessary to consolidate material support by family.
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Idoso , Humanos , Autoimagem , Inquéritos e QuestionáriosRESUMO
PURPOSE: This study was done to identify the degree that perceived social support effects the self esteem of the elderly. METHOD: A structured questionnaire was administered to 203 elderly subjects from April 2003 to June 2003. The data were analyzed with an SPSS program for descriptive statistics, Pearson`s correlation coefficients, t-test, ANOVA, and stepwise multiple regression. RESULTS: The level of social support was moderate; family support received the highest score among sources of support. In types of support, appraisal support was the highest score and material was the lowest. The level of self esteem was also moderate. The score of social support showed a slightly high positive correlation with self esteem. In general characteristics, several variables were significantly related to self esteem. The most powerful predictor of self esteem was material support by family and the variance explained was 19.6%. A combination of material support by family, informational support by relatives, perceived economic status, perceived health status, and having a subject to depend on accounted for 39.8% of the variance in self esteem of the elderly. CONCLUSION: To increase the self esteem of the elderly, it is necessary to consolidate material support by family.
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Idoso , Humanos , Autoimagem , Inquéritos e QuestionáriosRESUMO
The primary cause of tooth loss after 30 years of age is periodontal disease. Destruction of alveolar bone by periodontal disease is done by bone resorbing activity of osteoclasts. Understanding differentiation and activation mechanism of osteoclasts is essential for controling periodontal disease. The purpose of this study is to identify the possible effects of Vitamin D and cytokines affecting osteoclasts and its precursor cells. Four to six week-old mice were killed and humerus, radius, tibia and femur were removed aseptically and washed two times with Hank's solution containing penicillin-streptomycin and then soft tissue were removed. Bone marrow cells were collected by 22 gauge needle. Cells were cultured in Hank's solution containing 1 mg/ml type II collagenase, 0.05% trypsin, 4mM EDTA. Supernatant solution was removed 5 times after 15 minutes of digestion with above mentioned enzyme solution, and remained bone particles were maintained in alpha-MEM for 15 minutes and 4degrees C temperature. Bone particles were agitated for 1 minute and supernatant solution containing osteoclast precursor cells were filtrated with cell stainer. These separated osteoclast precursor cells were dispensed with 100-mm culture dish by 1x10(7) cells unit and cultured in alpha-MEM containing 20 ng/ml recombinant human M-CSF, 30 ng/ml recombinant human soluble osteoclast differentiation factor and 10% fetal calf serum for 2 and 7 days. Total RNA of osteoclast precursor cells were extracted using RNeasy kit. One microgram of total RNA was reverse transcribed in 42degrees C for 30 minutes using SuperScriptII reverse transcriptase. Expression of transcribed receptors of each hormone and cytokine were traced with 1 microliter of cDNA solution by PCR amplification. Vitamin D receptor was found in cells cultured for 7 days. TNF-alphareceptor was found in cells cultured for 2 days and amount of receptors were increased by 7 days. IL-1 type I receptor was not found in cells cultured 2 and 7 days. But, IL-1 receptor type II was found in cells cultured for 2 days. TGF-alpha,betatype I receptor was found in cells cultured 2 and 7 days, and amount of receptors were increased by 7 days of culture. These results implies Vitamin D and cytokines can affect osteoclasts directly, and affecting period in differentiation cycle of osteoclasts is different by Vitamin D and cytokines.
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Animais , Humanos , Camundongos , Células da Medula Óssea , Colagenases , Citocinas , Digestão , Di-Hidroergotamina , DNA Complementar , Ácido Edético , Fêmur , Úmero , Interleucina-1 , Fator Estimulador de Colônias de Macrófagos , Agulhas , Osteoclastos , Doenças Periodontais , Reação em Cadeia da Polimerase , Rádio (Anatomia) , Ligante RANK , Receptores de Calcitriol , RNA , DNA Polimerase Dirigida por RNA , Tíbia , Perda de Dente , Tripsina , Vitamina D , VitaminasRESUMO
Death is the critical moment of life. All elderly are faced with death. Therefore, the elderly's notion of death must be include in holistic care for the elderly. The purpose of this study was to understand the elderly's notion of death. The research questions were "What is the elderly's notion of death?", and "What is the embedded meaning of the elderly's notion of death?". This study was conducted using an ethnographic approch. The fieldwork for this study was done from July 1996 to July 1998 on an island in Chonbuk province. Data were collected by indepth interviews and participant observation. The result of this study is as follows The elderly's notion of death was founded on 'Being a ancestor god', 'Evaluation of one's own life', 'Be sujected to the evil(BuJung : the taboo of uncleanness)' 'Being a ancestor god' means the notion of death leading to eternal life. It means a relationship to this world, So, it is required for all descendants, especialy the son. Also, they thought that the Spirit is greater importance mote thait the body, and that the spirit is embedded in bone. And so, the spirit is delivered to descendants through a propitious site for grave(MoungDang). It is thought toa change in the fortune of the descendants(Balbok). 'Evaluation of ons's own life' means that life was evaluated personally and socially throughout the dy ing process. This means reflection on an individual's life and interpersonal relationship's, and appearance social aspects of the individual death. 'BuJung(be subjected to the evil)' emerged as fear about death, and pursuit of the cause of death, Most of the causes were attributed to supernatural entities. It contributed to the inspiration of a cornmunity identity. The elderly's notion of death was the present world oriented and actualized. This study contributed to the nurse's understanding of the Korean elderly's notion of death. Implications for nursing practice, education, and research were discussed. Further research at other settings and using other research methods were recommended.
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Idoso , Humanos , Causas de Morte , Educação , Enfermagem , TabuRESUMO
This study was designed to identify the relationship between perceived social support and discernd powerlessness held by the elderly residing in a rural community. The purpose of this study was to contribute to theoretical understanding of the relationship of these two variables and eventually to the more effective adaptation of the elderly to their situation. The subjects for this study were the 89 elderly residing in a rural community. Data were collected by a questionnaire from June 2 to 20, 1998. The data were analysed by descriptive statistics, t-test, ANOVA, Pearson correlated coefficient and stepwise multiple regression using a SAS program. The results of this study can be summarized as follows. 1. The relationship between perceived social support and discernd powerlessness was significant(r = -.23502, p=.0266). Especially, discernd elderly's powerlessness shows a highly significant relationship with family and neighborhood support(family r= -.45096, p=.0001, neighborhood r= -.35681, p=.0006). In the support patterns, the discernd powerlessness of the elderly has a significant relationship to emotional, informational and evaluational support. Therefore the hypothesis that, "the lower the degree of social support peiceived by the elderly, the higher the degree of discened powerlessness" was supported. 2. Stepwise multiple regression analysis revealed that the most powerful predictor of discened powerlessness was family support in support system (R2 = .2034), and emotional support (R2 = .0627) in support patterns. 3. General characteristic related to the degree of social support was only residential status(P< .05), but the degree of family support was related to spouse(t=2.390. p<.01). residential status(t= -2.157, p<.05), and household.
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Idoso , Humanos , Características da Família , Características de Residência , População RuralRESUMO
This study was to find out the perception of toward death and caring behavior of lay parsons in one community : One Island in Pusan County, Chonbuk. The methodology of this study was ethnography. For this study, The fieldwork was conducted from October 1997 to July 1998. Data collected by in-depth interview and participant observations. The participants consisted of were 17 persons of both sexes. The key informants were four specific people. The result of this study is as follows; The people perceived two different kinds of death. Normal death, which means death from old age. The person was respected as an ancestor God and was believed to exist forever with their offspring. Abnormal death was regarded as negative, many had fears toward this king of death. The causes of abnormal death were supernatural phenomena and had absolute holy meanings. Whether death was good or bad, The death was not personal, but collective events as family or community affairs and was interpreted as death and birth for their offsprings. Funeral rites were family-centered and/or community-centered. The did normal procedures for normal deaths for abnormal deaths, there were many protective ceremonies(BuJungmagi : the prevention of the taboo of uncleanliness) for the remaining people. These ceremonies combined confucism and shamanism. Caring behavior for dying persons was ruled as community-centered, reciprocal and reality-centered principles.