RESUMO
Topoisomerase II beta (Topo IIß) is one of the two isoforms of type II topoisomerases present in higher eukaryotes. This 180 kDa nuclear protein involves in different cellular processes like transcription, recombination, etc., apart from its normal topological functions. Previously, we have reported the association of this isoform along with the other isoform topoisomerase II alpha (Topo IIα) with HIV-1 reverse transcription complex and the downregulation of Topo IIß expression resulted in incomplete reverse transcription. In this study, we have tested the Topo IIß specific siRNA delivery using protein nanoparticles prepared with c-terminal domine of transferrin (c-ter) for the first time. Results show that, c-ter nanoparticles resemble apotransferrin nanoparticles in drug holding capability and drug delivery but with small in size. Topo IIß specific siRNA delivered in the form of c-ter nanoformulation resulted in knockdown of Topo IIß expression for the prolonged periods and which intern resulted in decreased viral replication of HIV-1.