Effects of Edible Insects on the Mycelium Formation of Cordyceps militaris.

<b>Background and Objective:</b> <i>Cordyceps militaris </i>is a potential edible medicinal mushroom which containing various biological activity such as anti-inflammatory, anti-ageing, anti-protozoal and anti-microbial. The compositions of <i>C. militaris</i> media were composed of carbon source, nitrogen source and other additives. This research aimed to evaluate the effect of edible insects on the <i>C. militaris </i>mycelium formation. <b>Materials and Methods:</b> Seven edible insects including <i>Bombyx mori </i>L., <i>Samia ricini</i> D., <i>Acheta domesticus</i> L., <i>Gryllus bimaculatus</i> De Geer, <i>Tenebrio molitor</i> L., <i>Rhynchophorus ferrugineus</i> and <i>Lethocerus indicus</i> were used as nitrogen source supplemented in Potato Dextrose Agar (PDA) and the mycelium formation of each edible insects at day 7, 14 and 21 were recorded. <b>Results:</b> The results of nitrogen source from a boiled edible insect at day 21 indicated that the highest colony diameter at 88.00 mm was obtained when cultured with PDA+<i>B. mori</i> L. The results of nitrogen source from a dried edible insect at day 21 presented that the highest diameter at 84.33 mm was obtained from cultured using PDA+<i>A. domesticus</i> L. <b>Conclusion:</b> The suitable boiled and dried edible insects for the supplement in PDA were boiled <i>B. mori</i> L. and dried <i>A. domesticus </i>L. This is the first report about PDA supplemented with edible insects that can be increased the <i>C. militaris</i> mycelium formation which the initial stage that important for <i>C. militaris </i>cultivation.

Rapid and sensitive detection of Vibrio vulnificus by loop-mediated isothermal amplification combined with lateral flow dipstick targeted to rpoS gene.

A novel loop-mediated isothermal amplification (LAMP) combined with amplicon detection by chromatographic lateral flow dipstick (LFD) assay was developed and evaluated for the detection of Vibrio vulnificus. Biotinylated LAMP amplicons were produced by a set of six designed primers that recognized the V. vulnificus RNA polymerase subunit sigma factor S (rpoS) gene followed by hybridization with an FITC-labeled probe and LFD detection. The optimized time and temperature conditions for the LAMP assay were 90 min at 65 °C. The LAMP-LFD method accurately identified 14 isolates of V. vulnificus but did not detect 25 non-vulnificus Vibrio isolates and 37 non-Vibrio isolates. The sensitivity of LAMP-LFD for V. vulnificus detection in pure culture was 1.5 × 10(3) CFU ml(-1) or equivalent to 2.8 CFU per reaction. In the case of spiked oyster samples without enrichment, the detection limit for V. vulnificus was 1.2 × 10(4) CFU g(-1) or equivalent to 11 CFU per reaction. The results show that this method appears to be accurate, precise and valuable tool for identification of V. vulnificus and can be used efficiently for detection of V. vulnificus in contaminated food sample.