Sodium azide inhibition of microbial activities and impact on sludge floc destabilization.

Absence of sludge deflocculation under prolonged (24 h or longer) conditions with dissolved oxygen (DO) less than 0.5 mg L⁻1 was recently reported. The reduced aerobic microbial activity, was speculated, had been compensated by the activity of other bacterial (i.e. facultative) communities. To assess such a compensation mechanism and to better evaluate impact of overall microbial activity on the flocculation process, SBR sludge samples were inhibited by using sodium azide under various DO conditions. Sludge deflocculated only in the presence of sodium azide, regardless of DO conditions. This was linked to sodium azide's inhibitory effects on the microbes as indicated by the reduced ammonium and DOC removals. Extracellular potassium level in the mixed liquor of azide spiked samples also indicated simultaneous cell lysis. Fluorescence excitation emission matrix (FEEM) analysis of the extracted bound EPS and fluorescence quenching based interaction studies indicated sodium azide had interacted with the EPS components, and especially with the bound EPS proteins. The impact of such interactions on reduced floc stability needs consideration. This study confirmed the importance of overall microbial activity in the biological flocculation process and the role of bacterial communities, other than the aerobes, in mitigating deflocculation under low DO conditions.

Understanding and optimization of the flocculation process in biological wastewater treatment processes: A review.

In the operation of biological wastewater treatment processes, fast sludge settling during liquid-solids disengagement is preferred as it affects effluent quality, treatment efficiency and plant operation economy. An important property of fast settling biological sludge is the ability to spontaneously form big and dense flocs (flocculation) that readily separates from water. Therefore, there had been much research to study the conditions that promote biological sludge flocculation. However, reported findings have often been inconsistent and this has possibly been due to the complex nature of the biological flocculation process. Thus, it has been challenging for wastewater treatment plant operators to extract practical information from the literature. The aim of this review is to summarize the current state of understanding of the factors that affect sludge flocculation so that evaluation of such information can be facilitated and strategize for intervention in the sludge flocculation and deflocculation process.