Green Synthesis of Copper and Copper Oxide Nanoparticles From Brown Algae Turbinaria Species' Aqueous Extract and Its Antibacterial Properties.

Background Copper and copper oxide nanoparticles synthesized by green methods have attracted considerable attention due to their environmentally friendly properties and potential applications. Green synthesis involves non-hazardous and sustainable techniques used in the production of a wide range of substances, including nanoparticles, pharmaceuticals, and chemicals. These methods often use different organisms, including bacteria, fungi, algae, and plants, each offering different advantages in terms of simplicity, cost-effectiveness, and environmental sustainability. The environmentally friendly nature of these green synthesis methods responds to the growing need for sustainable nanotechnologies. Brown algae have gained popularity due to their distinct morphological characteristics and diverse biochemical composition. This research focuses on the process of synthesizing copper and copper oxide nanoparticles from the brown algae Turbinaria. It emphasizes the natural ability of the bioactive compounds contained in the algae extract to reduce and stabilize the nanoparticles. The green synthesis of copper and copper oxide nanoparticles from brown algae has demonstrated a wide range of applications, including antibacterial activity. Materials and methods Fresh Turbinaria algae were collected from marine environments to ensure that they were free of contaminants. The algae underwent a purification process to remove impurities and were dried. An aqueous extract was prepared by pulverizing the dried algae and mixing them with distilled water. A copper salt solution utilizing copper nitrate was prepared. The algae extract was mixed with the copper salt solution. There are bioactive compounds in the algae extract that help reduce copper ions, which makes copper and copper oxide nanoparticles come together. The reaction mixture was incubated in a controlled environment to facilitate the growth and enhance the stability of the nanoparticles. To separate the nanoparticles from the reaction mixture, centrifugation was employed, or filtration was done with Whatman filter paper (Merck, Burlington, MA). The nanoparticles were dried to yield a stable powder. Results Copper and copper oxide nanoparticles derived from brown algae extract showed antibacterial effects against Streptococcus mutans, Klebsiella sp., and Staphylococcus mutans. The scanning electron microscopy (SEM) analysis verified the irregular shape and elemental content of the synthesized copper and copper oxide nanoparticles. The X-ray diffraction (XRD) analysis indicated that the synthesized nanoparticles exhibited a crystallinity nature and were composed of a mixture of copper and copper oxide species, namely face-centered cubic and monoclinic structures. The transmission electron microscopy (TEM) images showed copper and copper oxide nanoparticles that were evenly distributed and had a rectangular shape. They exhibited substantial antimicrobial activity against both Gram-positive and Gram-negative bacteria. Conclusions This study enhances the field of green synthesis techniques by showcasing the adaptability of Turbinaria brown algae to synthesize copper and copper oxide nanoparticles. It underscores the potential advantages of these nanoparticles in terms of their antibacterial properties.

The Oncolytic Effect of Aerva lanata on Osteosarcoma Cell Lines via the Apoptotic Signaling Pathway.

Introduction Osteosarcoma, a malignant bone tumor, poses significant treatment challenges, necessitating the development of alternative therapeutic strategies. Aerva lanata (A. lanata), a medicinal plant with traditional use in various healthcare systems, has anti-cancer properties. This study looks at the oncolytic effect of A. lanata extract on osteosarcoma cell lines (sarcoma osteogenic-Saos2). Aim The aim of this study was to investigate the oncolytic effect of Aerva lanata on Saos2 cell lines through the apoptotic signaling pathway. Materials and methods A. lanata extract was prepared using Soxhlet extraction, and its cytotoxic effects on Saos2 cells were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Real-time polymerase chain reaction (RT-PCR) analysis of gene activity was used to assess the extract's effect on apoptotic signaling pathways. Results The MTT assay demonstrated a dose-dependent decrease in Saos2 proliferation following treatment with A. lanata extract at concentrations ranging from 50 µg to 200 µg. The standard deviations observed ranged from 1.414 to 7.071. Gene expression analysis revealed that the extract led to a reduction in the messenger ribonucleic acid (mRNA) levels of the anti-apoptotic marker B-cell lymphoma 2 (Bcl2), with standard deviations ranging from 1 to 0.535. Conversely, it induced an increase in the mRNA levels of the tumor suppressor protein p53, with standard deviations ranging from 1 to 1.835. These findings suggest that the extract modulates the apoptotic pathways of the Bcl2 and p53 genes.  Conclusion A. lanata extract exhibits promising anti-cancer activity against Saos2 osteosarcoma cell lines, inducing apoptosis by downregulating Bcl2 and increasing p53. The study's findings suggest that A. lanata may be useful as a natural treatment for osteosarcoma.

Anti-inflammatory Properties of Strontium Oxide Nanoparticles Synthesized From Suaeda monoica Saltmarsh.

Background Currently, nanotechnology is a rapidly advancing field of research. Because of their nanoscale dimensions, nanoparticles (NPs) find application in a wide range of industries, including engineering and medicine. The leaves of Suaeda monoica have anti-inflammatory qualities. The purpose of this study was to create SrO NPs isolated from the leaves of S. monoica aqueous extract and to evaluate their anti-inflammatory efficacy. The S. monoica saltmarsh, commonly known as South-Indian Seepweed, is a mangrove-associated plant and has been used as traditional medicine for decades with multifunctional biological activity. Objectives The aim of our study is to biosynthesize strontium oxide NPs from S. monoica saltmarsh and to see whether they have any anti-inflammatory properties. Materials and methods In the present study, the pharmacological significance was studied using crude extract and synthesized SrO NPs from S. monoica. The synthesized SrO NPs were characterized using UV spectrophotometry. The in vitro anti-inflammatory assay was analyzed using egg albumin denaturation. SrO NPs' peak observance was found at 630 nm, and a graph was plotted for the zone of inhibition vs concentration and compared with the standard.  Results It was observed that the color of the SrO NPs deepened during the synthesis process. Furthermore, at a wavelength of 630 nm, the UV spectrum analysis showed a noteworthy absorption value of 1.4. The activity of inflammatory enzymes is significantly impacted by the anti-inflammatory properties of SrO NPs in the protein denaturation inhibition test. Conclusions The application of SrO NPs in the synthesis process has the potential to enhance the anti-inflammatory activity of Suaeda monoica as evidenced by the observed increase in anti-inflammatory capacity and defense against infections and injury.

Sensing of Quercetin With Cobalt-Doped Manganese Nanosystems by Electrochemical Method.

BACKGROUND: The pressing need for precise, quick, and affordable detection of diverse biomolecules has led to notable developments in the realm of biosensors. Quercetin, a biomolecule rich in flavonoids predominantly found in our diet, is sensed by the electrochemical method. The electrochemical properties show remarkable improvement when Mn2O3 (MO) is doped with cobalt (Co).  Aim: This study aimed to investigate the biomolecule sensing of quercetin using Co-doped MO by electrochemical method.  Materials and methods: Co-doped MO nanospheres were prepared by hydrothermal method. The crystal structure of the synthesized material was evaluated by using X-ray diffraction analysis. The sample morphology was assessed by using field emission scanning electron microscopy (FE-SEM) techniques. The cyclic voltammetry technique was used for the detection of quercetin biomolecules.  Results: The synthesized Co-doped MO appeared to be spherical in morphology in FE-SEM. Energy-dispersive X-ray spectroscopy showed the only presence of Co, Mn, and O, which confirmed the purity of the sample. The modified electrode sensed the biomolecule with a higher current of 7.35 µA than the bare glassy carbon electrode of 6.1 µA. CONCLUSION: The Co-doped MO exhibited enhanced conductivity, reactivity, and electrochemical performance. This tailored approach will help in the optimization of material properties toward specific biomolecule sensing applications.

Isolation of Secondary Metabolites From Marine Actinobacterium of Microbispora sp.T3S11 and Their Antibacterial Activities.

Introduction Marine actinobacteria are promising sources of novel bioactive compounds due to their distinct ecological niches and diverse secondary metabolite production capabilities. Among these, Microbispora sp. T3S11 is notable for its unique spore chain structure, which allows for both morphological and genetic identification. Despite its potential, little is understood about the secondary metabolites produced by this strain. In this study, we hope to fill this gap by extracting and analyzing the antibacterial activities of secondary metabolites from Microbispora sp. T3S11, which will be the first time its bioactive compound profile is investigated. Aim To evaluate the antibacterial activity of secondary metabolites isolated from the marine actinobacterium Microbispora sp. T3S11. Materials and methods The antibacterial assays were carried out on agar plates containing the appropriate media for each pathogen. Sterile filter paper disks were impregnated with secondary metabolites extracted from Microbispora sp. T3S11 and placed on the surface of agar plates inoculated with the appropriate pathogens. Similarly, disks containing tetracycline were used as a positive control. The plates were then incubated at the appropriate temperature for each pathogen, and the zones of inhibition around the disks were measured to determine the extracted metabolites' antibacterial activity. Result Secondary metabolites had antimicrobial activity against Streptococcus mutans, Klebsiella pneumonia, and methicillin-resistant Staphylococcus aureus (MRSA). The inhibition of S. mutans was 7.5 mm and 8.5 mm at 75 µg/mL and 100 µg/mL, respectively. Klebsiella pneumonia zones measured 7 mm and 7.5 mm, while MRSA zones measured 7.6 mm and 8.5 mm at the same concentrations. Tetracycline, the standard antibiotic, had larger inhibition zones: 22 mm for S. mutans and Klebsiella pneumonia and 16 mm for MRSA, indicating variable susceptibility. Conclusion We conclude that the secondary metabolites extracted from Microbispora sp. T3S11 exhibits high antibacterial activity. This could be attributed to the presence of various active compounds.

Computational Analysis of Single Nucleotide Polymorphisms in Human HIC1 Gene.

Background A putative tumor suppressor gene called HIC1 (hypermethylated in cancer) is situated at 17p13.3, a locus where the allelic loss occurs often in human malignancies, including breast cancer. Hypermethylated in cancer 1 protein is a protein that in humans is encoded by the HIC1 gene and it's a Homo sapiens (Human). This gene functions as a growth regulatory and tumor repressor gene. The molecular function of HIC1 gene includes DNA-binding transcription factor activity, sequence-specific DNA binding, DNA binding, histone deacetylase binding, protein binding, metal ion binding, nucleic acid binding, DNA-binding transcription repressor activity, RNA polymerase II-specific, DNA-binding transcription factor activity, RNA polymerase II-specific. The biological process of HIC1 gene includes multicellular organism development, negative regulation of Wnt signaling pathway, positive regulation of DNA damage response, signal transduction by p53 class mediator regulation of transcription, DNA-templated, negative regulation of transcription by RNA polymerase II, Wnt signaling pathway, transcription, DNA-templated, intrinsic apoptotic signaling pathway in response to DNA damage, cellular response to DNA damage stimulus. The study aimed to predict the stability and structure of the protein that will arise from single nucleotide polymorphisms (SNPs) in the human HIC1 gene. Methodology To investigate the possible negative effects associated with these SNPs, bioinformatic analysis is typically essential. The following tools were employed for forecasting harmful SNPs: scale-invariant feature transform (SIFT), Protein Analysis Through Evolutionary Relationships (PANTHER), nonsynonymous SNP by Protein Variation Effect Analyzer (PROVEAN), and nonsynonymous SNP by Single Nucleotide Polymorphism Annotation Platform (SNAP). Results The present study identified a total of 36 SNPs using the SIFT approach, which were shown to have functional significance. Twenty-six were determined to be tolerable, whereas 10 were shown to be detrimental. Out of 20 SNPs, seven (P370A, P646S, R654P, A476T, S400S, D666N, D7V) SNPs were predicted as "Possibly damaging" and seven (L9F, G468R, G490R, L482R, S12W, G489D, S12P) were identified as "probably benign", and six (R725G, G620S, A56V, E463D, D394N, L338V) were identified as "probably damaging" according to the predictions made by PANTHER tools. The majority of the pixels on the strip were red, indicating that the gene changes may have dangerous consequences. These results highlight the need for more research to fully comprehend how these mutations affect the hic1 protein's function, which is essential for the emergence of different types of cancer. Conclusion The current research has provided us with essential information about how SNPs might be used as a diagnostic marker for cancer, given that SNPs may be candidates for cellular changes caused by mutations linked to cancer.

Merremia emarginata Extract Potentiates the Inhibition of Human Colon Cancer Cells (HT-29) via the Modulation of Caspase-3/Bcl-2-Mediated Pathways.

Background This study investigates Merremia emarginata's curative effectiveness against colon cancer cells. M. emarginata, often known as Elika jemudu, is a Convolvulaceae family plant. The inhibitory ability of anticancer herbal extracts against cancer cell growth and mediators is tested.  Aim This study aims to evaluate the potent anticancer activity of M. emarginata against colon cancer cell line (HT-29). Materials and methods M. emarginata leaves were gathered and processed using solvent extraction. Anticancer activity on colon cancer cells was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and cysteine aspartic acid protease-3 (caspase 3), B-cell lymphoma 2 (Bcl-2), and B-cell lymphoma-extra large (Bcl-xL) mRNA expressions. The data was reported as the mean ± SD of three separate experiments done in triplicate. The statistical analysis was carried out using one-way analysis of variance (ANOVA), with a p-value less than 0.05 indicating statistical significance. Results The cell viability test showed a gradual decrease in cell growth and proliferation as the concentration increased. The ethanolic extract of M. emarginata was found to be cytotoxic against colon caller cell lines. The extract was able to induce apoptosis of cancer as revealed by Bcl-2, Bcl-xL, and caspase-3 (p<0.05 and p<0.001) signaling pathways. Conclusion M. emarginata extracts showed good anticancer activity against colon cancer cell lines. Further work is required to establish and identify the chemical constituent responsible for its anticancer activity.

Synthesis of Zinc Oxide Nanoparticles From Cymodocea Serrulata Leaf Extract and Their Biological Activities.

Introduction The utilization of Cymodocea serrulata for the eco-friendly synthesis of zinc oxide nanoparticles, which contain distinguishable nanostructures, presents a cost-effective and environmentally sustainable alternative for producing zinc nanoparticles. The production process of zinc nanoparticles are rich in phytochemicals, which can serve as stabilizing and reducing agents. Zinc nanoparticles can easily pass through bacterial cell walls and reach all cellular components. C. serrulata, is a small submerged angiosperm commonly found in submerged and tidal coastal environments. Aim Analysis of the biological activities of zinc oxide nanoparticles made from C. serrulata leaf extract. Materials and Methods Dry leaves of C. serrulata were ground into a powder, which was then placed into a conical flask and filled with water. Subsequently, the color of the mixture turned black. Next, a 20 mm piece of ZnO was dissolved in a 60 ml sample of distilled water to prepare the metal solution. Following this, a wavelength scan ranging from 200 to 700 nm was conducted using ultraviolet (UV) spectroscopy. After shaking the solution for an hour, a final reading was taken across the UV spectrum. The synthetic sample should also be centrifuged to remove any pellets and subsequently dried in a hot air oven. Result Using nanoscale profiling, the average particle size was measured and found to be less than 100 nm, specifically UV spectrum analysis revealed a notable absorbance value of 47.0 nm, at different angles within the peak height. The wavelength range of the zinc nanoparticles was observed to be between 250 and 350 nm. Conclusion The antibacterial properties of ZnO NPs have been demonstrated through in vitro investigations, indicating their potential application in in vivo studies.

Biosynthesis of Zinc Nanoparticles From Actinobacterium Streptomyces Species and Their Biological Potential.

BACKGROUND: In today's world, antibiotic-resistant microorganisms are a major concern. There is solid evidence that metal nanoparticles (NPs) tend to have antimicrobial properties. The most effective substitute for antibiotic resistance is the incorporation of metal NPs. The antibacterial properties of NPs are currently being explored and shown to be successful. Zinc (Zn) NPs that are biosynthesized from marine Actinobacterium proved to be more biocompatible, bioactive, and affordable.  Aim: This study aims to investigate the synthesis of ZnNPs from Actinobacterium Streptomyces species and their antimicrobial effects against gram-positive and gram-negative bacteria. MATERIALS AND METHODS: The current study uses natural, considerably safer processes to synthesize ZnNPs from marine Actinobacteria with little to no negative side effects. It involves sample collection, identification, and isolation of Actinobacterium Streptomyces species. The isolated sample was air-dried, and extracts of ZnNPs were taken. Among the isolates from marine sediment, two Actinobacteria that generate bioactive secondary metabolites-Streptomyces species (MOSEL-ME28) and Rhodococcus rhodochrous (MOSEL-ME29)-were selected for extracellular synthesis of ZnNPs. The antimicrobial activity of the biosynthesized ZnNPs from marine Actinobacteria was analyzed against Staphylococcus (MRSA), Klebsiella pneumoniae, and Streptococcus mutans. The results were statistically analyzed and graphs were created. RESULTS: ZnNPs obtained from Actinobacterium Streptomyces species exhibited antimicrobial effects against Staphylococcus (MRSA), Klebsiella, and Streptococcus mutans. At 280 nm wavelength, analysis of the UV spectrum showed a notable absorbance value of 1.8. The antibacterial efficacy against Staphylococcus MRSA, Klebsiella species, and Streptococcus mutans was assessed by measuring the zone of inhibition in diameter. The zones of inhibition were 8, 8, and 7 mm on the evaluation for Streptococcus mutans, S. aureus, and Klebsiella species, respectively, at a dose of 75 µg/mL. When the dosage was increased to 100 µg/mL, the inhibition zones were found to be 9.5, 9, and 7.5 mm for the respective bacterial strains. CONCLUSION: ZnNPs are biosynthesized from marine Actinobacterium Streptomyces species in this research study. They have a significant antimicrobial activity against both gram-positive and negative bacteria. This indicates that ZnNPs have enormous antimicrobial potential and have an extensive spectrum of applications. However, clinical trials must be completed before it can be used safely on patients.

Development of the Antioxidant Property of Seagrass Extract-Based Hydrogel for Dental Application.

Background Seagrass is rich in antioxidants, which can help neutralize harmful free radicals in the oral cavity. Free radicals can contribute to oxidative stress, inflammation, and various oral health issues. Incorporating seagrass extract into a hydrogel can enhance its antioxidant capacity, providing a protective effect for oral tissues. The hydrogel, composed of a biocompatible base, ensures that the material is well-tolerated by oral tissue. This is crucial for any dental application to avoid adverse reactions. Aim This work aimed to develop an antioxidant hydrogel that incorporates seagrass extract, with a specific emphasis on its possible use in dentistry. Methods A seagrass sample was collected, and its bioactive compounds were extracted through the utilization of methanol, and subsequent filtration was done. The resulting seagrass filtrate was then integrated into a hydrogel, which was synthesized using polyacrylamide and sodium alginate. Antioxidant hydrogel underwent testing for antioxidant activity through both the 2,2-diphenyl-1-picrylhydrazyl assay and the 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) assay. Besides, the hydrogel functional groups were investigated using Fourier transform infrared spectroscopy, while its crystalline structure was examined using X-ray diffraction analysis. Conclusion Seagrass extract provides inherent antioxidant properties, and incorporating this bioactive extract into the hydrogel imparts antioxidant features. The hydrogel's controlled-release property ensures both safety and efficiency. Antioxidant hydrogel for dental applications holds the potential to improve oral health.

Biosynthesis of Silver Nanoparticles From Marine Actinobacterium Micromonospora sp. and Their Bioactive Potential.

Background The biosynthesis of nanoparticles represents a rapid, environmentally friendly, cost-effective, and straightforward technology. This approach allows for the production of nanoparticles with a wide range of chemical compositions, sizes, shapes, high uniformity, and scalability. One of the principal advantages of biogenic nanoparticles is their water solubility and compatibility with biological systems. Biologically synthesized nanoparticles have demonstrated superior efficiency compared to conventionally synthesized particles. Among biosynthesis, microbial-mediated biosynthesis is a promising one that has a selectively reducing ability on specific metal ions through electron transfer.  Objectives Evaluation of antimicrobial and antioxidant activity of silver nanoparticle synthesized by actinobacteria Micromonospora sp. which is isolated from marine environment. Materials and methods In this study, actinobacteria were isolated from the marine sediment using the spread plate method. The isolates were identified based on morphological observation, cell wall amino acids, sugar analysis, and micromorphological analysis. The silver nanoparticle synthesis from microbes and their inhibition against clinical pathogens have been evaluated by the disc diffusion method. Antioxidant efficiency was evaluated in terms of total antioxidant activity through ammonium molybdenum assay. Results A total of five isolates were isolated from the sediment sample. The cell-free extract of MBIT-MSA4 can synthesize silver nanoparticles that have potential antimicrobial activity against the clinical pathogens Streptococcus mutans at a zone of inhibition 6 mm, 10 mm inhibition zone of Klebsiella pneumonia, and 8 mm zone of inhibition of Staphylococcus aureus. Also, it has significant antioxidant activity up to 73% of free radical inhibition. Conclusion Marine microbial-mediated biosynthesized silver nanoparticles have potential antimicrobial activity against S. mutans and methicillin-resistant Staphylococcus aureus (MRSA) and inhibit the oxidation process through antioxidant activity. This enhanced efficient biosynthesised nanoparticle has significantly reduced the concentration of free radicals caused by pathogens.

Anticancer Effects of Rosmarinus officinalis Leaf Extract on KB Cell Lines.

Background The value and use of medicinal plants, including the widespread cultivation of Rosmarinus officinalis, have increased rapidly. R. officinalis, a medicinal plant native to the Mediterranean, has received attention for its potential therapeutic benefits. This study evaluates R. officinalis anticancer activity using human epithelial carcinoma (KB) cell lines derived from nasopharyngeal epidermoid carcinoma. The KB cell line is known for its increased sensitivity to specific chemotherapeutic agents (CA), making it a useful model in cancer research. The impact of R. officinalis is assessed using comprehensive analyses of cell viability and gene expression. Aim This study aims to evaluate the anti-cancer effects of R. officinalis on KB cell lines. Materials and methods The R. officinalis leaf extract was separated and used to treat KB cell lines. The cell viability of treated KB cells was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Real-time polymerase chain reaction (RT-PCR) was used to analyze the expressions of matrix metalloproteinase (MMP-9) and tumor-inducing metalloproteins (TIMP-1) messenger ribonucleic acid (mRNA) genes. The statistical analysis was performed. Results This study analyzes the anticancer properties of R. officinalis on KB cell lines. The results show that increasing the concentration of rosemary extract reduces cell viability in malignant cells. Furthermore, the R. officinalis effect on the apoptotic signaling system is demonstrated by a decrease in MMP-9 and TIMP-1 mRNA expressions, as observed by RT-PCR analysis. Conclusion Patients looking for natural anticancer treatments may benefit from biogenically prepared anticancer drugs. The current research focuses on R. officinalis as a potential alternative to chemically synthesized anticancer drugs.

Evaluation of Antibacterial and Anti-oxidant Activities of Suaeda monoica Extract for Its Potential Application.

BACKGROUND:  An assessment of Suaeda monoica extract's antimicrobial and antioxidant properties was undertaken in light of its possible application as an oral care product. The maintenance of optimal dental health is just as important as overall wellness. Food particles become trapped in the mouth cavity, making it easy for oral bacteria to infect. AIM:  The study sought to ascertain the antibacterial and antioxidant properties of salt marsh Suaeda monoica extract. MATERIALS AND METHODS:  Leaves of Suaeda monoica, collected, dried and powdered, were dissolved in 70% methanol and the extract of 25-100 µg/ml was analyzed for antioxidant activity through total antioxidant assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and total reducing power. Suaeda monoica antibacterial activity was also performed and the minimum inhibitory concentration was determined for 75 µg/ml, 100µg/ml, and 150 µg/ml concentrations and tetracycline in 10mcg/disc as a control against three different oral pathogens: Staphylococcus mutans, Streptococcus aureus, and Klebsiella spp. RESULTS:  At varying concentrations of 75 mg/ml to 150 mg/ml, Suaeda monoica extracts are efficacious with varying concentrations against the investigated bacterial strains. In the present study, in the DPPH assay, total reducing power, and total antioxidant activity assay, there was an increase in inhibitory percentage as the concentration increased from 25-100 µg/ml, showing maximum inhibition at 100 µg/ml concentration. CONCLUSION:  The results of the investigation show that Suaeda monoica has significant antibacterial and antioxidant activity in a concentration-dependent manner and can be potentially used as an oral care agent after it is assessed for clinical use.

Fabrication and Partial Characterization of Silver Nanoparticles From Mangrove (Avicennia marina) Leaves and Their Antibacterial Efficacy Against Oral Bacteria.

BACKGROUND: Scientists are currently investigating ecologically sound and enduring techniques for nanoparticle production. Utilizing natural sources such as plant extracts provides an environmentally friendly and economically efficient method. Avicennia marina, also referred to as the gray mangrove, is predominantly located in coastal regions. The leaves of this plant may contain bioactive metabolites that can be used to synthesize nanoparticles. OBJECTIVES: This study aimed to synthesize silver nanoparticles (AgNPs) using A. marina leaf extract and subsequently assess their antibacterial properties against oral pathogens. MATERIALS AND METHODS: The present research involved the successful synthesis of AgNPs using an environmentally sustainable method employing the leaf extract of A. marina. The reduction of Ag ions to AgNPs was confirmed using UV-visible spectroscopy. This analytical technique revealed the presence of a distinct surface plasmon resonance peak at approximately 420 nm, which is indicative of the formation of AgNPs. Fourier transform infrared spectroscopy (FTIR) operating within the frequency range of 500-3500 cm-1 and scanning electron microscopy (SEM) morphology of the image indicated agglomeration of the nanoparticles, with distinct particles ranging from 10 to 20 nm and dense rod-shape, which was carried out from Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu, India. In energy-dispersive spectroscopy (EDS), a strong signal and maximum formation percentage were received at 42.7%, assigned to the element silver. RESULTS: AgNPs showed significant antibacterial efficacy against both gram-positive bacteria, including Staphylococcus aureus and Streptococcus mutans, and gram-negative bacteria, such as Klebsiella sp. In general, the use of A. marina leaf extract for the green synthesis of AgNPs is a viable and environmentally friendly approach for producing nanoparticles that exhibit favorable biological properties. Consequently, these nanoparticles hold considerable appeal as potential candidates for a range of biomedical applications, particularly as antibacterial agents. CONCLUSION: The synthesis of AgNPs using A. marina leaf extract shows great potential in the field of creating nanomaterials that are compatible with biological systems and is promising for a wide range of clinical applications. Nevertheless, it is imperative to conduct comprehensive scientific research and rigorous clinical trials to effectively apply these discoveries to real-world medical interventions, while prioritizing patient safety and therapeutic effectiveness.

Computational Exploration of Single-Nucleotide Polymorphisms in the Human hRAS Gene: Implications and Insights.

Background A group of genes called oncogenes includes the Harvey rat sarcoma virus (hRAS) gene. Along with hRAS, Kirsten rat sarcoma viral oncogene homolog (kRAS) and neuroblastoma RAS viral oncogene homolog (nRAS) genes belong to the Rat sarcoma (Ras) family of oncogenes. These three genes result in Rho guanosine triphosphate hydrolases (GTPases) as their protein product. Instructions for producing the protein hRAS, which is mainly involved in controlling cell division, are provided by the hRAS gene. The hRAS protein transfers signals from outside through a process called signal transduction. Because the hRAS protein is a GTPase, it changes the chemical guanosine-5'-triphosphate (GTP) into guanosine diphosphate (GDP). GTP and GDP molecules operate as switches to turn on and off the hRAS. This study aimed to anticipate the structure and stability of the protein resulting from missense single-nucleotide polymorphisms (SNPs) in the human hRAS genes. Methodology To investigate the possible negative effects associated with these SNPs, bioinformatic analysis is typically essential. The following tools were employed for forecasting harmful SNPs: Scale-Invariant Feature Transform (SIFT), Protein Analysis Through Evolutionary Relationships (PANTHER), non-synonymous SNP by Protein Variation Effect Analyzer (PROVEAN), and non-synonymous SNP by Single Nucleotide Polymorphism Annotation Platform (SNAP). Results The present study identified a total of 11 SNPs using the SIFT approach, which were shown to have functional significance. Only two of these 11 SNPs were determined to be tolerable, whereas nine were shown to be detrimental. Among the 11 SNPs analyzed, seven (Q61H, Q99H, K117R, A121D, A146V, R169W, R169Q) were classified as possibly damaging,and four (G13V, Q22K, Q61K, Q13V) were categorized as probably benign according to the predictions made by PANTHER tools. Therefore, the seven SNPs were identified as high-risk SNPs. Conclusions Given that SNPs have the potential to be candidates for cellular alterations brought on by mutations that are associated with cancer, this study provides vital information about how SNPs might be utilized as a diagnostic marker for cancer.

Biosynthesis of Copper Nanoparticles From Seaweed Ulva lactuca and Their In Vitro Antioxidative Potential.

BACKGROUND: Marine macroalgae is consumed by individuals in several regions, including Scandinavia, Great Britain, Ireland, China, and Japan; in Japan, it is commonly referred to as aosa. Copper nanoparticles are primarily composed of copper and exhibit a size distribution ranging from 1 to 100 nm. Copper nanoparticles can be synthesized using chemical or natural means, similar to other nanoparticle variants. The nanoparticles in question have garnered significant attention owing to their historical utilization as coloring agents, as well as their contemporary applicability in medicine and antibacterial treatments. OBJECTIVES: The objective of this study was to investigate the biosynthesis of copper nanoparticles derived from Ulva lactuca seaweed and explore their in vitro antioxidative potential. MATERIALS AND METHODS: Seaweed samples (10 g) were mixed with 50 ml of distilled water and placed in a shaker for two days. Copper sulfate (10 mM) was mixed with 100 ml of distilled water to obtain a copper (Cu) solution. Cu nanoparticles were then synthesized by adding the aqueous extract to 100 ml of the Cu solution and mixing it in an orbital shaker at 180 rpm for 24 hours. They were observed both visually and via ultraviolet (UV) spectrophotometry to confirm their nanoparticle synthesis. The initial reading was performed using a UV-visible spectrometer at 300-800 nm. The sample was centrifuged at approximately 8000 rpm for 15 minutes, the pellet was removed, and the pellet was dried in a hot-air oven. The synthesized Cu nanoparticles were then investigated using in vitro antioxidant assays. RESULT: The seaweed-derived copper nanoparticles exhibited a 1.2 peak absorbance at 580 nm. Various concentrations of copper nanoparticles (25, 50, 75, and 100 µg/ml) were tested for free radical scavenging. As the copper nanoparticle concentration increased, the scavenging ability on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals assay showed that the free radical scavenging activity increased in a dose-dependent manner. Similar to the DPPH assay, the total antioxidant and hydrogen peroxide (H2O2)assays showed increased free radical scavenging with increasing concentration. CONCLUSION: The application of Cu nanoparticles in the synthesis process has the potential to enhance the antioxidant activity of Ulva lactuca as evidenced by the observed increase in antioxidant capacity and defense against reactive oxygen species.

Synthesis of Silver Nanoparticles From Cymodocea rotundata Leaf Extract and Their Biological Activities.

AIM: Silver nanoparticles (AgNPs) are considered to be a very significant and intriguing type within the category of metallic nanoparticles, particularly in the context of their involvement in biological applications. The objective of this research is to use the green synthesis method in order to synthesize AgNPs by using the leaf extract of C. rotundata. Furthermore, the study aims to evaluate the antioxidant and anti-inflammatory properties of these nanoparticles. MATERIALS AND METHODS: Fresh and healthy specimens of C. rotundata were gathered from Palk Bay, Tamil Nadu, India, and afterward subjected to a thorough washing process using tap water. The cleaned materials were air-dried and then fragmented into small bits and finely ground. The ethanolic extract of seagrass was then combined with a solution containing 1 millimolar (mM) silver nitrate (AgNo3). The decrease of silver ions in the solution was frequently measured using a UV-visible spectrophotometer. Synthesized AgNPs were investigated for antioxidants by DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and anti-inflammatory activity was measured by protein-denaturation assay. RESULTS: The use of C. rotundata leaf extract in the green synthesis of AgNPs, in the presence of 1 mM AgNO3, led to a noticeable alteration in the colour of the mixture, transitioning from a pale hue to a brown shade. This change in colour serves as evidence of the reduction of AgNo3 ions to silver ions, thereby facilitating the creation of AgNPs. The duration of the bio-reduction process of silver ions in the reaction mixture was observed to be two hours. The antioxidant and anti-inflammatory activity showed promising activity for AgNPs. CONCLUSION: This study concluded that C. rotundata had antioxidant capabilities, and AgNPs derived from C. rotundata have potential use in pharmaceuticals and medication administration.

Synthesis of Zinc Oxide Nanoparticles From Aqueous Extract of Avicennia marina Mangrove Leaves and Their Antibacterial Activities Against Oral Pathogens.

Introduction The field of nanotechnology is currently being extensively researched. Nanoparticles (NPs) are used in many fields, such as engineering and medicine, owing to their nanoscale dimensions. Zinc (Zn) appears to be the most desirable metal NP, as it is being applied in various drug delivery systems and other fields. The green synthesis of the NPs used in this study makes it affordable and nonpolluting. Avicennia marina leaves possess antimicrobial properties and a high secondary metabolite content. This study aimed to synthesize ZnO NPs from the aqueous extracts of A. marina mangrove leaves and assess their antibacterial activities against oral pathogens. Methodology The leaves of A. marina were dried to obtain a preprocessed powder, and from that, an aqueous extract was prepared. ZnO NPs were then synthesized by adding the aqueous extract to 100 mL of ZnS solution and mixing it in an orbital shaker. They were observed both visually and by ultraviolet (UV) spectrophotometry to confirm their synthesis. The antibacterial properties of these ZnO NPs were assayed using the disc diffusion method on three different oral bacterial strains (Streptococcus mutans, Staphylococcus aureus, and Klebsiella sp.).  Results For the synthesis process, it was seen that zinc oxide (ZnO) NPs exhibited a deepening in coloration. Additionally, the UV spectrum analysis revealed a notable absorbance value of 1.2 at a wavelength of 320 nm. The antibacterial efficacy against S. mutans, S. aureus, and Klebsiella sp. was assessed by measuring the zone of inhibition in diameter. At a dosage of 100 µg/mL of ZnO NPs, the inhibition zones were found to be 7.5 ± 0.2, 9.5 ± 0.5, and 9.5 ± 1.2 mm for S. mutans, S. aureus, and Klebsiella sp., respectively. Similarly, at a concentration of 75 µg/mL, the inhibition zones were measured to be 7 ± 0.25, 9 ± 1, and 7.5 ± 0.5 mm for the respective bacterial strains. Conclusions This study synthesizes ZnO NPs using A. marina leaf aqueous extract in a sustainable and eco-friendly manner. The ZnO NPs' antibacterial activities against oral infections indicate their use in dental products. These NPs have promising potential for nanomedicine and oral health studies due to their antibacterial properties and ecologically sustainable manufacturing.

Evidence linking the association of periodontal viruses and pentraxins in periodontitis patients with coronary artery disease.

BACKGROUND: Although there is ample evidence in the literature supporting a significant positive association between key periodontal pathogens and established inflammatory markers of periodontitis and coronary artery disease (CAD), their exact role remain unclear. Especially, the role of viruses in the etiology and specific biomarkers have not been validated. Thus, the current study aims to evaluate the role of periodontal viruses such as Epstein-Barr virus (EBV), cytomegalovirus (CMV), and herpes simplex virus (HSV), as well as the inflammatory marker pentraxin-3 (PTX3), and to analyze their association with CAD. METHODS: The study included 240 patients divided into four groups of 60 patients each: nonperiodontitis + noncardiac (NP+NC) group, periodontitis + noncardiac patients (P+NC) group, nonperiodontitis + cardiac patients (NP+C) group, and periodontitis + cardiac (P+C) group. The cardiac surgery group (C-S) was a subgroup of NP+C and P+C. It consisted of 60 patients from the abovementioned two cardiac groups in whom coronary artery bypass graft (CABG) was indicated. Demographic variables, cardiac parameters, and periodontal parameters were recorded. The viruses (EBV, CMV, and HSV) and the inflammatory marker PTX3 were evaluated in the subgingival plaque samples of all the four groups and atheromatous plaque samples of the C-S using reverse transcriptase-polymerase chain reaction (RT-PCR) and quantitative polymerase chain reaction (qPCR), respectively, and were compared between the groups. The results were obtained and statistically analyzed. RESULTS: The demographic variables did not differ significantly between the groups, except for age. Systolic blood pressure, diastolic blood pressure, low-density lipoprotein, and random blood sugar were significantly higher in NP+C and P+C, whereas high-density lipoprotein was significantly lower (p ≤ 0.05) in the same. Plaque index (PI), probing pocket depth (PPD), and clinical attachment level (CAL) were significantly higher (p ≤ 0.05) in P+NC and P+C. PTX were significantly elevated in P+C among the four groups. On evaluating the subgingival plaque samples, EBV and CMV were significantly higher in the two periodontitis groups P+NC and P+C (p = 0.000). HSV was significantly higher in the two cardiac groups (NP+C and P+C) (p ≤ 0.05). Cardiac EBV and CMV were significantly elevated in the P+C group with a p value of 0.004 and 0.033, respectively. Cardiac HSV was found in the NP+C group with statistical insignificance (p = 0.410) between the groups. On correlation, oral PTX were significantly associated with bleeding index (BI), PPD, and CAL (p = 0.000). Similarly, cardiac PTX showed significant association with PI, BI, PPD, and CAL (p = 0.000). Oral and cardiac PTX also showed significant correlation with each other. Multiple logistic regression analysis revealed a significant association between CAL and oral EBV (p ≤ 0.05). Similarly, cardiac EBV showed a significant association with CAL and oral EBV (p ≤ 0.05). Multiple logistic regression analysis also revealed that both cardiac and oral PTX showed a significant association only with oral EBV, CMV, and HSV. CONCLUSION: The results of the current study suggest that the clinical severity of periodontitis (CAL), etiology of periodontitis (EBV and CMV), and inflammatory marker of periodontitis (PTX3) were found to be significantly elevated in CAD. These findings suggests that periodontal diseases may be a risk factor that could influence the progression of CAD.

Evaluation of the periodontal viruses in cardiovascular patients associated with periodontitis.

OBJECTIVES: The current study aimed in evaluating the prevalence of Epstein-Barr virus (EBV), cytomegalovirus (CMV), and herpes simplex virus (HSV) in periodontitis and/or coronary artery disease (CAD) patients to compare with their healthy controls and insist their significance in the same. METHODOLOGY: Two hundred and forty patients were divided into 4 groups. Non-periodontitis+non-cardiac (NP+NC) = 60 patients, periodontitis+non-cardiac patients (P+NC) = 60 patients, non-periodontitis+cardiac patients (NP+C) = 60 patients, and periodontitis+cardiac (P+C) = 60 patients. Demographic variables, cardiac and periodontal parameters were recorded. EBV, CMV, and HSV were evaluated in the subgingival plaque samples using RT-PCR (real-time polymerase chain reaction) and compared between the groups. The results were statistically analyzed using Student's t-test, Pearson's chi-square, Turkey post hoc analysis, and multiple logistic regression analysis. RESULTS: The demographic variables did not differ significantly between the groups, except for age. Systolic blood pressure, diastolic blood pressure, low-density lipoprotein, and random blood sugar were significantly higher in NP+C and P+C (p ≤ 0.05). The plaque index, probing pocket depth, and clinical attachment loss (p ≤ 0.05) were significantly higher in P+NC and P+C. EBV and CMV were significantly higher in the two periodontitis groups P+NC and P+C (p-value = 0.000). HSV was significantly higher in the two cardiac groups (NP+C and P+C) (p≤0.05). Multiple logistic regression analysis revealed a significant association between EBV and CAL (p ≤ 0.05). CONCLUSION: The study concluded that higher prevalence of EBV and CMV was found in groups with periodontitis patients. This indicates the significant role of the viruses in periodontitis as confirmed by association between EBV and CAL. The viruses were said to be highest in periodontitis patients with CAD. This could pave a new link in the risk of CAD in periodontitis patients.