Biodegradable calcium carbonate carriers for the topical delivery of clobetasol propionate.

Inflammatory dermatoses represent a global problem with increasing prevalence and recurrence among the world population. Topical glucocorticoids (GCs) are the most commonly used anti-inflammatory drugs in dermatology due to a wide range of their therapeutic actions, which, however, have numerous local and systemic side effects. Hence, there is a growing need to create new delivery systems for GCs, ensuring the drug localization in the pathological site, thus increasing the effectiveness of therapy and lowering the risk of side effects. Here, we propose a novel topical particulate formulation for the GC clobetasol propionate (CP), based on the use of porous calcium carbonate (CaCO3) carriers in the vaterite crystalline form. The designed carriers contain a substantially higher CP amount than conventional dosage forms used in clinics (4.5% w/w vs. 0.05% w/w) and displayed a good biocompatibility and effective cellular uptake when studied in fibroblasts in vitro. Hair follicles represent an important reservoir for the GC accumulation in skin and house the targets for its action. In this study, we demonstrated successful delivery of the CP-loaded carriers (CP-CaCO3) into the hair follicles of rats in vivo using optical coherent tomography (OCT). Importantly, the OCT monitoring revealed the gradual intrafollicular degradation of the carriers within 168 h with the most abundant follicle filling occurring within the first 48 h. Biodegradability makes the proposed system especially promising when searching for new CP formulations with improved safety and release profile. Our findings evidenced the great potential of the CaCO3 carriers in improving the dermal bioavailability of this poorly water-soluble GC.

Rapid Ultrasound Optical Clearing of Human Light and Dark Skin.

Application of optical clearing of biological tissue in humans in vivo is challenging due to toxicity of chemical agents, long processing time (≥30 min), and moderate (1.3-1.5-fold) imaging depth improvement. Here, we introduce novel, robust, and rapid ultrasound-based optical clearing of human skin without chemical agents that provides dramatic (up to 10-fold) reducing processing time down to 2-5 min. We discovered that ultrasound alone can increase a light depth penetration for optical coherence tomography (OCT) up to ~1.5-fold during 2 min. Nevertheless, sequent application of microdermabrasion, oleic acid and ultrasound allowed increasing OCT signal amplitude up to 3.3-fold with more than twice improved depth penetration during 30 min that was not demonstrated with other approaches. Comparison of these effects in light and dark skin revealed similarity of the optical clearing mechanisms. However, for combined optical clearing, only 1.34-fold increase in OCT signal amplitude was achieved for dark skin.