Data on community structure and diversity of the intestinal bacteria in elver and fingerling stages of wild Indonesian shortfin eel (Anguilla bicolor bicolor).

This article describes the data on community structure and diversity of intestinal bacteria of Indonesian shortfin wild eel fingerling and elver (Anguilla bicolor bicolor). The specimens were obtained from Ci Kangean River, Alur Village, Cipatujah District, Tasikmalaya, West Java, Indonesia. The data were generated using DNA metagenomic approach on an Illumina paired-end platform by utilizing the V3-V4 region of the 16S rRNA gene. A total of 151,636 reads with 91.60% effective tags and 155,388 with 84.86% effective tags were generated from the intestine of wild eel fingerling (WF) and elver (WE), respectively. The total OTUs was 100 in WF and 358 in WE. The phyla Bacteroidetes (50%), Firmicutes (15%), Proteobacteria (13%), Fusobacteria (11%) and Verrucomicrobia (8%) were found in WF, and Proteobacteria (64%), Firmicutes (34%) and Fusobacteria (1%) were found in WE. The predominating families in WF were Porphyromonadaceae (50%), Clostridiaceae (12%), Fusobacteriaceae (10%), Verrucomicrobiaceae (8%), and in WE were Enterobacteriaceae (38%), Clostridiaceae (30%), Aeromonadaceae (17%), Moraxellaceae (7%). The predominating genera in WF were unassigned (48%), Cetobacterium (10%), Clostridium (sensu stricto) (9%), Akkermansia (8%), Odoribacter (4%), Bacteroides (4%), Desulfovibrio (4%), and in WE were Plesiomonas (36%), Clostridium (sensu stricto 1) (31%), Aeromonas (17%), Acinetobacter (7%). The amount of lactic acid bacteria found in the intestine of WF was 0.0028% and WE was 0.1218%. The data provide baseline information on the changes in the community and bacterial composition in line with the stages of growth and development of wild Indonesian shortfin eels.

Characterization of pyrene and chrysene degradation by halophilic Hortaea sp. B15.

Polycyclic aromatics hydrocarbons (PAHs) are ubiquitous and toxic pollutants that are dangerous to humans and living organism in aquatic environment. Normally, PAHs has lower molecular weight such as phenanthrene and naphthalene that are easy and efficient to degrade, but high-molecular-weight PAHs such as chrysene and pyrene are difficult to be biodegraded by common microorganism. This study investigated the isolation and characterization of a potential halophilic bacterium capable of utilizing two high-molecular-weight PAHs. At the end of the experiment (25-30 days of incubation), bacterial counts have reached a maximum level (over 40 × 1016 CFU/mL). The highest biodegradation rate of 77% of chrysene in 20 days and 92% of pyrene in 25 days was obtained at pH 7, temperature 25 °C, agitation of 150 rpm and Tween 80 surfactant showing to be the most impressive parameters for HMWPAHs biodegradation in this research. The metabolism of initial compounds revealed that Hortaea sp. B15 utilized pyrene to form phthalic acid while chrysene was metabolized to form 1-hydroxy-2-naphthoic acid. The result showed that Hortaea sp. B15 can be promoted for the study of in situ biodegradation of high molecular weight PAH.