Managing insect and plant pathogen pests with organic and conventional pesticides in onions.

Onion thrips (Thrips tabaci Lindeman, Thysanoptera: Thripidae) is a significant insect pest of onions (Allium cepa L., Asparagales: Amaryllidaceae). In addition to feeding on onion foliage, they may spread plant pathogens. Currently, onion thrips and pathogens are managed as separate pests with insecticides and fungicides. It may be beneficial to manage these pests simultaneously as limiting onion thrips may reduce pathogen damage. We tested combinations of bio- and conventional pesticides in a season-long management program in Michigan onion fields. From 2020 to 2022, we counted onion thrips weekly and visually estimated plant foliage necrotic damage (%) in experimental plots each year. In 2020, we tested 6 treatment programs including: azadirachtin, spinosad, a copper-based fungicide, azadirachtin + copper-based fungicide, spinosad + copper-based fungicide, and untreated control. The thrips populations were not significantly reduced compared to the control, but necrotic damage was reduced significantly in spinosad-treated plots. In 2021, we tested a combination of 8 bio- and conventional pesticide programs. Compared to the control, the bioinsecticides did not reduce onion thrips populations, but the conventional pesticide programs reduced both onion thrips numbers and necrotic damage. In 2022, we tested only conventional insecticide programs but included 3 different action thresholds for initiation and applied them with or without a fungicide, for 8 treatments. All insecticide programs reduced onion thrips compared to the control, the action threshold did not impact thrips numbers significantly. Overall, the use of action thresholds can lead to fewer insecticide applications and a lower incidence of leaf damage.

Onion Thrips (Thysanoptera: Thripidae) Host Plant Preference and Performance are Mediated by a Facultative Plant Pathogen of Onion.

Insect vector and phytopathogen interactions are mediated by host plants. Insects interact with pathogens directly or indirectly and they may prefer host plants based on infection status. Performance on infected hosts varies depending on the type of pathogen involved. Species specific studies of economically important insects and phytopathogens are needed to understand how these interactions impact crop yields. Onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae), is an economically devastating insect pest of onions (Allium cepa L., Asparagales: Amaryllidaceae) worldwide and it co-occurs simultaneously with many different pathogens. Colletotrichum coccodes (Wallr) (Glomerellales: Glomerellaceae) is a generalist fungal pathogen that attacks onion foliage, causing tan lesions and decreasing yield. Onion thrips and C. coccodes represent two important pests of onions, but the relationship between onion thrips and C. coccodes infected onions has not been studied, and it is unclear if onion thrips contribute to the spread of C. coccodes in onion fields. A four-choice test with control, artificially injured, artificially injured + symptomatic, and inoculated-symptomatic onion suggests that onion thrips distinguish between hosts based on health status. Furthermore, a two-choice test with control, inoculated-asymptomatic, and inoculated-symptomatic onion pairings revealed that onion thrips distinguish between hosts based on infection status and prefer inoculated-symptomatic hosts. In a no-choice test, onion thrips numbers increased on inoculated-symptomatic plants compared to control or inoculated-asymptomatic plants. Overall, we found that onion thrips preferred and performed best on C. coccodes infected plants.

Beneficial and Pest Arthropod Responses to Tillage and Cover Crop Residues in Organic Cucurbits.

Reduced tillage methods such as strip tillage are often combined with cover crop mixtures to provide agronomic benefits which can support crop and soil health. However, reduced tillage and cover crop species/arrangements effects on arthropods is less understood and results of previous studies have varied. In this study, we examined how agriculturally relevant pest and beneficial arthropod species were impacted by tillage and cover crop methods in USDA-certified organic Cucurbita (L.) (Cucurbitales: Cucurbitaceae). Aphididae were the most observed foliar pests and abundance was highest in full tillage treatment plots while foliar herbivores overall, excluding Aphididae, were more abundant in strip tillage treatment plots regardless of cover crop arrangement. Formicidae was also observed more on foliage, flowers, and in pitfall traps in full tillage, similarly to Aphididae. Parasitica were the most observed foliar natural enemies with increased abundance in strip tillage treatments. Activity densities of several epigeal natural enemies were also higher in strip tillage compared to full tillage. No significant effects of tillage and cover crop treatments were observed on flower visitation rates of Eucera pruinosa (Say) (Hymenoptera: Apidae), an important pollinator of Cucurbita, while the highest visitation rates of Apis mellifera (L.) (Hymenoptera: Apidae) and Bombus spp. (Latreille) (Hymenoptera: Apidae) were in strip tillage treatments. These results suggest that reduced tillage methods can support greater abundances of natural enemies and possibly pollinators. This may lead to enhanced biological control and pollination, but impacts may vary for different arthropod species and crops.

Heat of the moment: extreme heat poses a risk to bee-plant interactions and crop yields.

Extreme heat events threaten the development, functioning, and success of bee pollinators and crops that rely on pollinators for high yields. While direct effects of extreme heat and climate warming have gained more attention, the indirect effects on bees and crops remain largely unexplored. Extreme heat can directly alter the nutritional value of floral rewards, which indirectly contributes to lower bee survival, development, and reproduction with implications for pollination. Phenological mismatches between bee activity and crop flowering are also expected. Heat-stressed crop plants with reduced floral rewards may reduce bee foraging and nesting, limiting pollination services. Understanding how extreme heat affects bee-crop interactions will be essential for resilient production of pollinator-dependent crops in this era of climate change.

A newly invasive species may promote dissimilarity of pest populations between organic and conventional farming systems.

Insect taxa vary in their phenology across space creating dissimilar patterns of species abundance over time. The role of human disturbances and invasive species in these patterns of temporal dissimilarity (phenological differences) across space, however, remain largely unexplored. To dissect these patterns, we evaluated four common pests and one newly invasive species (Contarinia nasturtii; Swede midge) at 220 time points across 2 years on organic and conventional farms. We first summarized across time and evaluated differences in pest abundance between farm management (organic and conventional). We then used generalized additive models to describe temporal patterns of abundance, disentangling phenological differences across management systems. Last, we conducted a temporal beta diversity analysis to identify which species and management practices contribute most to dissimilarity. We found that aggregating pest abundance across time and species masked differences in pest phenology across management systems and concealed variation in pest abundance that was strongly driven by an invasive species, respectively. Overall, our results suggest that organic and conventional farms may be only superficially similar in pest abundance. Rather, by accounting for time, we demonstrate a more nuanced understanding of pest communities moving beyond abundance that may be particularly important for management of newly invasive species.

A Meta-analysis of Interactions Between Insect Herbivores and Plant-Parasitic Nematodes.

Insect herbivores and plant-parasitic nematodes are global, economically devastating pests that are present in nearly every crop and natural system worldwide. Although they may be spatially separated, they indirectly interact with each other by altering both plant chemical defense and nutrition. However, the outcome of these interactions is highly variable across different focal species. We performed a meta-analysis to determine how plant and nematode traits influence insect herbivore growth and reproduction, as well as nematode abundance and reproduction. We investigated how interactions between plant-parasitic nematodes and insect herbivores influence plant biomass, carbon, and nitrogen in the roots and shoots. We found no overall effect of nematodes on insect herbivores or insect herbivores on nematodes. However, while phloem-feeding insect reproduction was not affected by nematode feeding guild or plant family, chewing insect growth increased in the presence of cyst nematodes and decreased in the presence of gall nematodes. The effect of nematodes on chewing insect herbivore growth was also affected by the focal plant family. Nematode presence did not alter plant biomass when plants were exposed to aboveground insect herbivory, but carbon and nitrogen were higher in roots and nitrogen was higher in shoots of plants with nematodes and insects compared to plants with insects alone. Our results indicate that the mechanisms driving the outcome of aboveground-belowground interactions are still unclear, but those chewing insects may have more variable responses to nematode damage than phloem-feeders.

Identifying Leafhopper Targets for Controlling Aster Yellows in Carrots and Celery.

Aster yellows phytoplasma (Candidatus Phytoplasma asteris) is a multi-host plant pathogen and is transmitted by at least 24 leafhopper species. Pathogen management is complex and requires a thorough understanding of vector dynamics. In the American Midwest, aster yellows is of great concern for vegetable farmers who focus on controlling one vector, Macrosteles quadrilineatus-the aster leafhopper. However, vegetable-associated leafhopper communities can be diverse. To investigate whether additional species are important aster yellows vectors, we surveyed leafhopper communities at commercial celery and carrot farms in Michigan from 2018 to 2019 and conducted real-time PCR to determine infection status. Leafhoppers were collected within crop fields and field edges and identified with DNA barcoding. Overall, we collected 5049 leafhoppers, with the most abundant species being M. quadrilineatus (57%) and Empoasca fabae-the potato leafhopper (23%). Our results revealed the most abundant aster yellows vector in Michigan in both crops is M. quadrilineatus, but we also found that E. fabae may be a potential vector for this pathogen. While several taxa reside in and near these crops, we did not find strong evidence that they contribute to phytoplasma infection. These findings indicate that M. quadrilineatus should be the primary target for controlling this pathogen.

Predation threat modifies Pieris rapae performance and response to host plant quality.

Predators impact prey populations through both consumptive and non-consumptive effects, such as behavioral and physiological changes by prey in response to a predation threat. Additionally, various top-down (e.g. predator characteristics) and bottom-up factors (e.g. plant nutrients) may impact non-consumptive effects, yet little is understood about how these interact. We studied how host-plant choice, leaf consumption, and growth of an herbivore, Pieris rapae, were impacted by different levels of plant nitrogen (N) and two predator species representing varying degrees of threat, Hippodamia convergens (predator of early-instars) and Podisus maculiventris (predator of all-instars). We found that P. rapae adults and larvae made similar choices about bottom-up and top-down factors when threatened by two different predator species. Adults and larvae preferred high N plants when threatened by H. convergens, but plant N did not influence their host plant choice when threatened by P. maculiventris. Additionally, larvae consumed more leaf tissue and grew larger when threatened by H. convergens, but leaf tissue consumption and larval growth did not change under threat by P. maculiventris, suggesting that larvae may change their behavior if they are able to quickly outgrow life stages vulnerable to predation. These results indicate that top-down factors such as predator identity may determine how P. rapae modulate their responses to bottom-up factors such as host plant quality when utilizing anti-predator behaviors.

A novel plant pathogen management tool for vector management.

BACKGROUND: Decision support systems often focus on insect control due to direct damage. However, when insects vector plant pathogens, these decision support systems must be tailored to disease management. However, a decision system that links diagnosticians to vector management is lacking and complicated by patterns of insect abundance over space and time. Here, we describe an approach that integrated monitoring of an insect pest (aster leafhopper; Macrosteles quadrilineatus, Forbes) that vectors aster yellows phytoplasma (Candidatus Phytoplasma spp.), with rapid disease diagnostics and web-based text messaging in two crops, carrots and celery. RESULTS: From 2014-2019, a total of 8,343 aster leafhoppers were collected, 99 of these were infected with phytoplasma. Text messaging reduced the number of infected leafhoppers. When we compared infected leafhopper density across crops, their temporal patterns were most similar at a 2-week delay. Comparisons within crop indicated that in celery uninfected and infected leafhopper density was most similar at a 2-week delay, but there was no similar pattern in carrots. Leafhopper density and infectivity were not similar beyond individual farms. CONCLUSION: Our results suggest that farmers should account for these temporal and spatial patterns when managing leafhoppers infected with aster yellows phytoplasma to improve pest management. By combining extensive monitoring, with rapid disease diagnostics, and text messaging, we demonstrate the value of our decision support tool. © 2020 Society of Chemical Industry.

Citizen science improves our understanding of the impact of soil management on wild pollinator abundance in agroecosystems.

Native bees provide essential pollination services in both natural and managed ecosystems. However, declines in native bee species highlight the need for increased understanding of land management methods that can promote healthy, persistent populations and diverse communities. This can be challenging and costly using traditional scientific methods, but citizen science can overcome many limitations. In this study, we examined the distribution and abundance of an agriculturally important wild bee species, the squash bee (Eucera (Peponapis) pruinosa, Hymenoptera: Apidae). They are ground nesting, specialist bees that depend on cultivated varieties of Cucurbita (squash, pumpkins, gourds). The intimate relationship between squash bees and their host plants suggests that they are likely sensitive to farm management practices, particularly those that disturb the soil. In this study, citizen scientists across Michigan used a survey to submit field management and bee observation data. Survey results indicated that squash bees occupy a wide geographic range and are more abundant in farms with reduced soil disturbance. Citizen science provided an inexpensive and effective method for examining impacts of farm management practices on squash bees and could be a valuable tool for monitoring and conserving other native pollinators.

Biological Control of Asparagus Pests Using Synthetic Herbivore-Induced Volatiles.

Natural enemies can be important regulators of pests in agroecosystems, and they often rely on volatile chemical cues to find hosts. Herbivore-induced plant volatiles (HIPVs) have been a focal point in many studies that seek to increase the efficacy of biological control programs by increasing recruitment and retention of natural enemies. Our research sought to explore the role of HIPVs in asparagus agroecosystems to answer the following questions: 1) What is the composition of HIPV produced by asparagus ferns following feeding by a chewing herbivore? 2) Do field deployed lures baited with synthetic asparagus HIPVs attract natural enemies? and 3) Can HIPV lures increase biological control of asparagus pests? Volatiles were field collected from the headspace of healthy asparagus ferns, mechanically damaged ferns, and ferns fed upon by asparagus beetle larvae (Crioceris asparagi L.) (Coleoptera: Chrysomelidae). We found that asparagus beetle damaged asparagus had significantly higher concentrations of (E)-ß-ocimene, (E,E)-α-farnesene, and (1)-tetradecanol than healthy or mechanically damaged ferns. Field experiments demonstrated that lures baited with isolates of ocimene and farnesene attracted parasitoids without attracting pests, but had no impact on predator recruitment. Finally, we determined that overall parasitism rates were not increased by synthetic HIPV lures but found evidence that lures may increase parasitism of asparagus miner (Ophiomyia simplex Loew) (Diptera: Agromyzidae) by pteromalids.

Onion Thrips (Thysanoptera: Thripidae) Feeding Promotes Infection By Pantoea ananatis in Onion.

Onion thrips, Thrips tabaci Lindeman, is a primary insect pest of onions (Allium cepa) worldwide. Onion thrips cause feeding damage by destroying epidermal tissue. They are also vectors of Pantoea ananatis (Serrano) Mergaert, the bacteria that causes center rot. Onions with center rot develop white streaks with water-soaked margins along the onion leaves, which turn necrotic and lead to bulb rot during storage. The role of thrips feeding on the establishment and progression of bacterial infection in onions has not been investigated. Onions infested with thrips and inoculated with P. ananatis had more necrotic tissue and symptoms were more severe with increasing thrips density. We conducted a fluorescence microscopy study that examined how P. ananatis (expressing a fluorescence protein gene) colonized a control group of onions without thrips in comparison to a test group of onions with thrips. We found that P. ananatis colonized some onions in the control group because of naturally existing wounds in the epidermal tissue but more colonization was found in the thrips infested group because of the increased presence of entry points caused by thrips feeding. Overall, our results demonstrate that wounds caused by thrips feeding facilitate center rot development by providing entry sites for the bacteria into leaf tissue.

Bee Community of Commercial Potato Fields in Michigan and Bombus impatiens Visitation to Neonicotinoid-Treated Potato Plants.

We conducted a bee survey in neonicotinoid-treated commercial potato fields using bowl and vane traps in the 2016 growing season. Traps were placed outside the fields, at the field edges, and 10 and 30 m into the fields. We collected 756 bees representing 58 species, with Lasioglossum spp. comprising 73% of all captured bees. We found seven Bombus spp., of which B. impatiens was the only known visitor of potato flowers in our region. The majority of the bees (68%) were collected at the field edges and in the field margins. Blue vane traps caught almost four-times as many bees and collected 30% more species compared to bowl traps. Bee communities did not differ across trap locations but they were different among trap types. We tested B. impatiens visitation to neonicotinoid treated and untreated potato flowers in field enclosures. The amount of time bees spent at flowers and the duration of visits were not significantly different between the two treatments. Our results demonstrate that a diverse assemblage of bees is associated with an agroecosystem dominated by potatoes despite the apparent lack of pollinator resources provided by the crop. We found no difference in B. impatiens foraging behavior on neonicotinoid-treated compared to untreated plants.

Elucidating the Common Generalist Predators of Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae) in an Organic Apple Orchard Using Molecular Gut-Content Analysis.

Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae), plum curculio, is a serious direct pest of North American tree fruit including, apples, cherries, peaches and plums. Historically, organophosphate insecticides were used for control, but this tool is no longer registered for use in tree fruit. In addition, few organically approved insecticides are available for organic pest control and none have proven efficacy as this time. Therefore, promoting biological control in these systems is the next step, however, little is known about the biological control pathways in this system and how these are influenced by current mechanical and cultural practices required in organic systems. We used molecular gut-content analysis for testing field caught predators for feeding on plum curculio. During the study we monitored populations of plum curculio and the predator community in a production organic apple orchard. Predator populations varied over the season and contained a diverse assemblage of spiders and beetles. A total of 8% of all predators (eight Araneae, two Hemiptera, and six Coleoptera species) assayed for plum curculio predation were observed positive for the presence of plum curculio DNA in their guts, indicating that these species fed on plum curculio prior to collection Results indicate a number of biological control agents exist for this pest and this requires further study in relation to cultural practices.

Comparing qPCR and Nested PCR Diagnostic Methods for Aster Yellows Phytoplasma in Aster Leafhoppers.

The aster yellows phytoplasma (AYp) is a wall-less bacterium that causes damage in multiple crops. They are spread primarily by the aster leafhopper, Macrosteles quadrilineatus (Hemiptera: Cicadellidae). A total of 3,156 aster leafhoppers were collected during the 2014 and 2015 growing seasons in Michigan celery and carrot fields using sweep nets. The objective of this study was to test previously developed 16S rDNA phytoplasma gene primers to find the most reliable and least time-consuming method for AYp detection in leafhoppers. Nested polymerase chain reaction (PCR) was performed with universal primers P1/P7 and R16F2n/R16R2, and then, restriction enzymes AluI, MseI, and HhaI identified the phytoplasma to subgroup. Over the two years, 2.2% of samples were phytoplasma positive with nested PCR, classified in subgroups 16SrI-A or 16SrI-B. All samples were also tested with a TaqMan quantitative qPCR assay with universal phytoplasma primers and probe and 4.6% tested positive. A subset of samples were also tested with AYp-specific SYBR green qPCR, showing a >93% similarity between SYBR green and TaqMan qPCR assay results. The qPCR assays were more than two times faster than nested PCR. However, qPCR assays likely have specificity issues that need to be addressed before they can be used as a reliable method of detection for AYp in leafhoppers.

Spatial and Temporal Potato Intensification Drives Insecticide Resistance in the Specialist Herbivore, Leptinotarsa decemlineata.

Landscape-scale intensification of individual crops and pesticide use that is associated with this intensification is an emerging, environmental problem that is expected to have unequal effects on pests with different lifecycles, host ranges, and dispersal abilities. We investigate if intensification of a single crop in an agroecosystem has a direct effect on insecticide resistance in a specialist insect herbivore. Using a major potato pest, Leptinotarsa decemlineata, we measured imidacloprid (neonicotinoid) resistance in populations across a spatiotemporal crop production gradient where potato production has increased in Michigan and Wisconsin, USA. We found that concurrent estimates of area and temporal frequency of potato production better described patterns of imidacloprid resistance among L. decemlineata populations than general measures of agricultural production (% cropland, landscape diversity). This study defines the effects individual crop rotation patterns can have on specialist herbivore insecticide resistance in an agroecosystem context, and how impacts of intensive production can be estimated with general estimates of insecticide use. Our results provide empirical evidence that variation in the intensity of neonicotinoid-treated potato in an agricultural landscape can have unequal impacts on L. decemlineata insecticide insensitivity, a process that can lead to resistance and locally intensive insecticide use. Our study provides a novel approach applicable in other agricultural systems to estimate impacts of crop rotation, increased pesticide dependence, insecticide resistance, and external costs of pest management practices on ecosystem health.

The parasitoids of the asparagus miner (Diptera: Agromyzidae): field parasitism and the influence of food resources on life history.

The goals of this study were to identify pupal parasitoids of the asparagus miner, Ophiomyia simplex Loew (Diptera: Agromyzidae), and examine the effect of different diets and floral resources on the lifespan of adult asparagus miners and their parasitoids. We also measured the effect of parasitism on stem damage caused by the asparagus miner. The identity and abundance of the parasitoids of the asparagus miner were determined in asparagus fields in Michigan from weekly asparagus miner pupal collections during the 2010-2013 seasons. Twelve species of hymenopterous parasitoids were reared from asparagus miner pupae, including Chorebus rondanii (Giard) (Ichneumonoidea: Braconidae), 10 species in three families of Chalcidoidea, and one species of Bethylidae (Chrysidoidea), that represent new host records for the asparagus miner. C. rondanii and Thinodytes cephalon (Walker) (Pteromalidae) were the most common parasitoids. The effects of different diets and flowers on the lifespan of the pest and parasitoid adults were also evaluated. Buckwheat resulted in the shortest life span for the asparagus miner, whereas Riddell's goldenrod significantly increased its lifespan relative to the control. Parasitoid lifespan was doubled when individuals were fed sugar-rich diets. In the field, parasitoids preferred stems that contained more pupae and damage. The two most commonly reared parasitoids should be considered as targets for future conservation biological control efforts of the asparagus miner.

The development of the asparagus miner (Ophiomyia simplex Loew; Diptera: Agromyzidae) in temperate zones: a degree-day model.

BACKGROUND: The asparagus miner is a putative vector of Fusarium spp., which have been implicated in globally declining asparagus production. Growers currently apply broad-spectrum insecticides for the asparagus miner, but lack management guidelines for adequately controlling the pest. Our aims were (1) to determine the lower developmental threshold of the asparagus miner, (2) develop and validate a degree-day model describing its phenology, and (3) create a developmental time budget for the asparagus miner to help guide growers' management decisions. RESULTS: We found that the lower developmental threshold for the asparagus miner was 12.1 °C, and that the phenology of the asparagus miner could be reliably predicted over the course of a two-year study. Predictions from the model match well with previously published information on the bionomics of the asparagus miner, but fit better for sampling data collected from the midwestern and eastern United States than for the United Kingdom. The life cycle of the asparagus miner likely requires between 1500 and 2000 degree-days to complete; the longest developmental time requirement was for the pupal stagen CONCLUSION: This study provides tools for the targeted management of the asparagus miner by offering a degree-day model that may be used to predict its life stages in the north-eastern United States.

Patterns of spatial and temporal distribution of the asparagus miner (Diptera: Agromyzidae): implications for management.

The asparagus miner is an obligatory feeder on asparagus and a putative vector for pathogenic fungi implicated in the early decline of asparagus fields. To date, the distribution of the asparagus miner over space and time is poorly understood. Our study evaluated the spatial and temporal pattern of adult asparagus miners in commercial asparagus fields in Michigan in 2011 and 2012. We sampled adults and damage weekly during the growing season using yellow sticky traps outside, at the edge, and inside commercial fields. Yellow sticky traps at each trapping location were placed at the canopy and ground level to determine vertical distribution of adults. During the first generation, adults were more evenly distributed throughout the field. In the second generation, adults were more commonly found on the edge of the field. Overall, there was a greater percent of mining damage near the edge of the field. Additionally, three times as many asparagus miners were found in the canopy compared with ground-level traps. There were 12 times as many asparagus miner adults on edges bordered by another asparagus field than on ones bordered by forest. Taken together, our results indicate that while asparagus miner management in the beginning of the growing season should focus on the entire field, in the latter half of the season, growers could save money and resources by targeting miner adults at the edges of fields. Finally, conserving the remaining naturally forested landscape and planting borders of trees may help ameliorate pest pressure in asparagus fields.

Segregation and efficacy of the cry1Ia1 gene for control of potato tuberworm in four populations of cultivated potato.

The potato (Solanum tuberosum L.) cultivar 'SpuntaG2' contains a single copy of the Bacillus thuringiensis (Bt) cry1Ia1 gene and controls potato tuberworm (Phthorimaea operculella Zeller, Lepidoptera: Gelechiidae). Two potato cultivars and two breeding lines were crossed with SpuntaG2 creating four populations used to study cry1Ia1 segregation and efficacy. The cry1Ia1 gene segregated in each of the four populations with a 1:1 ratio. All progeny that were polymerase chain reaction positive for the cry1Ia1 gene had no surviving larvae and no leaf mining in detached leaf assays after 72 h. These results support previous evidence that SpuntaG2 carries a single copy of the cry1Ia1 gene and that transmission of the transgene from parent to progeny is not restricted and follows expected Mendelian segregation ratios. Based on detached leaf assays, the efficacy of the cry1Ia1 gene is retained through sexual transmission. If the SpuntaG2 cry1Ia1 insertion event is deregulated for commercial use, SpuntaG2 could be used for conventional breeding and the progeny carrying the SpuntaG2 event would also be available for commercial use.