Assuntos
Anticorpos Monoclonais/uso terapêutico , Doenças Cardiovasculares/prevenção & controle , Lipídeo A/imunologia , Choque Séptico/prevenção & controle , Animais , Pressão Sanguínea/efeitos dos fármacos , Doenças Cardiovasculares/etiologia , Cães , Ensaio de Imunoadsorção Enzimática , Hemodinâmica/efeitos dos fármacos , Imunoglobulina M/imunologia , Radioisótopos do Iodo , Camundongos , Camundongos Endogâmicos BALB C , Salmonella/imunologia , Choque Séptico/complicações , Choque Séptico/fisiopatologiaAssuntos
Anticorpos Antibacterianos/análise , Doadores de Sangue , Transfusão de Sangue , Endotoxinas/imunologia , Plasma/imunologia , Salmonella/imunologia , Anticorpos Antibacterianos/administração & dosagem , Criança , Feminino , Glicolipídeos/imunologia , Humanos , Imunoterapia/métodos , Lactente , Toxemia/imunologia , Toxemia/terapiaRESUMO
The interaction of 62 S. aureus clinical strains and, respectively, 20 and 17 isolated S. epidermidis and S. saprophyticus strains with human blood plasma fibronectin (FN) has been studied. The specific interaction of FN with bacteria has been evaluated simultaneously by the binding of 125I with FN (method 1), the FN-mediated agglutination of staphylococci (method 2) and the character of colonies formed in 0.15% agar medium containing FN (method 3). The data obtained in this investigation indicated that all S. aureus strains under study react with FN to a different extent. When evaluating the binding of FN with bacteria, the most pronounced correlation was observed between methods 1 and 3. None of the methods used in this investigation has revealed interaction between FN and S. epidermidis and S. saprophyticus strains under study. The authors suggest that a preliminary inference on the capacity of the isolated clinical strains of staphylococci for reaction with FN may be made from the character of colonies formed in 0.15% agar medium containing FN.
Assuntos
Fibronectinas/sangue , Staphylococcus/metabolismo , Humanos , Radioisótopos do Iodo , Ligação Proteica , Staphylococcus/crescimento & desenvolvimento , Staphylococcus aureus/crescimento & desenvolvimento , Staphylococcus aureus/metabolismo , Staphylococcus epidermidis/crescimento & desenvolvimento , Staphylococcus epidermidis/metabolismoRESUMO
One hundred and seven sessions of hemosorption were performed on 77 patients with severe ulcerative colitis. Clinically, improvement was demonstrated in the reduction of the signs of intoxication in 50 to 60 per cent of patients. Combination of hemosorption and anti-inflammatory medication allowed to achieve a remission or a marked improvement determined by clinical observation or endoscopy of 39 from 52 patients with a severe, total form of the disease, whereas toxic influence of sulfasalazine was controlled and extra-intestinal complications were weakened in 8 patients from 11. Thirteen patients were operated upon due to inefficiency of therapy. Hemosorption contributed to reduction of content of protein molecules with mean molecular weight (61 per cent), phenol (73 per cent), and endotoxin of gram-negative bacteria (50 per cent). Dynamics of acute phase reactants and humoral immune factors testifies to a weak anti-inflammatory action of hemosorption. Reduced levels of plasma protein, albumin, potassium and cholesterol were corrected or spontaneously returned to normal.
Assuntos
Colite Ulcerativa/terapia , Hemoperfusão , Complexo Antígeno-Anticorpo/isolamento & purificação , Colite Ulcerativa/sangue , Colite Ulcerativa/imunologia , Humanos , Sulfassalazina/efeitos adversos , Sulfassalazina/uso terapêutico , Toxinas Biológicas/sangue , Toxinas Biológicas/isolamento & purificaçãoRESUMO
The data on the biological properties of the culture fluid of Streptococcus strain sp. TOM-1606 are presented. The native preparation has been shown to possess the capacity for stimulating the rate of the clearance of the peritoneal cavity of mice from Staphylococcus aureus cells, strain MT-1, rif. r., found to be insensitive to the action of the above-mentioned preparation in vitro. The crude preparation produces a transitory bacteriostatic effect on the streptococcal and staphylococcal strains under study. The preparation produces a prolonged bacteriostatic effect only on Micrococcus luteus test strain. All these data suggest that the crude preparation contains at least two active principles.
Assuntos
Antibacterianos/isolamento & purificação , Streptococcus/metabolismo , Animais , Antibacterianos/farmacologia , Bacteriocinas , Camundongos , Micrococcus/efeitos dos fármacos , Resistência às Penicilinas , Penicilinas/farmacologia , Cavidade Peritoneal/microbiologia , Staphylococcus aureus/efeitos dos fármacos , Streptococcus pyogenes/efeitos dos fármacos , Fatores de TempoRESUMO
Diagnostic possibilities of selective examination of renal urine particularly collected under medicamentous polyuria conditioned by the administration of Lazix were studied in latent bacteriuria. By means of separate collection of renal urine against the background of polyuria it was possible additionally to detect bacteriuria in 1/3 of the patients, to record increase in the intensity of the index in almost half of the patients with renal bacteriuria and, in 1/4 of them to detect, in renal urine, the aetiological agent absent from bladder urine. The latter circumstance not only has a diagnostic significance but also plays a certain role in the selection of medicamentous therapy in chronic pyelonephritis. In addition to traditional bacteriological methods, filtration through membrane filters was used to isolate and identify microflora in the urine. By means of this method it is possible to detect extremely low bacteriuria which cannot be established by any other method.
Assuntos
Bactérias/isolamento & purificação , Bacteriúria/diagnóstico , Pielonefrite/urina , Bacteriúria/microbiologia , Bacteriúria/urina , Doença Crônica , Feminino , Humanos , Rim/microbiologia , Cálculos Renais/microbiologia , Cálculos Renais/urina , Pielonefrite/microbiologia , Bexiga Urinária/microbiologiaRESUMO
Active and passive immunization with heated and ribosomal vaccines prepared from the mutant of S. minnesota was found to enhance the inactivation of P. aeruginosa in the abdominal cavity of mice, but had no influence on the inactivation of staphylococci. The inactivating potency of antisera was due to the presence of IgM and IgG. Vaccines prepared from the S-forms of enterobacteria did not enhance the inactivation of P. aeruginosa.
Assuntos
Abdome/microbiologia , Vacinas Bacterianas/imunologia , Mutação , Pseudomonas aeruginosa/imunologia , Salmonella/imunologia , Animais , Imunização , Imunização Passiva , Imunoglobulina G/imunologia , Imunoglobulina M/imunologia , CamundongosAssuntos
Escherichia coli/genética , Plasmídeos , Antígenos de Bactérias/genética , Bacteriocinas/biossíntese , Conjugação Genética , DNA Bacteriano/genética , Escherichia coli/imunologia , Escherichia coli/patogenicidade , Fator F , Conformação Molecular , Peso Molecular , Fenótipo , Fatores R , Tolerância a Radiação , VirulênciaRESUMO
It was shown that mutation in the rfa region causing disturbances in the structure of the basal part of the polysaccharide of the cell wall or the absence of O-specific side chains led to the loss of protective activity of the ribosomal fractions isolated from the cells of the murine typhoid salmonella by sedimentation with dihydrostreptomycine sulphate. Ribosomal fractions isolated from the murine typhoid salmonella transductants with the replaced rfb region failed to protect the animals from the infection with the virulent. S. typhimurium, S. enteritidis cultures. The virulence of the mutants and transductants was also changed in comparison with the initial strains.
Assuntos
Polissacarídeos Bacterianos/biossíntese , Ribossomos/metabolismo , Salmonella typhimurium/patogenicidade , Transdução Genética , Animais , Lipopolissacarídeos/biossíntese , Camundongos , Mutação , Salmonella typhimurium/citologia , VirulênciaAssuntos
Enterotoxinas/biossíntese , Escherichia coli/metabolismo , Doença Aguda , Animais , Colite/etiologia , Diarreia/etiologia , Diarreia/microbiologia , Enterite/etiologia , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/complicações , Fezes/microbiologia , Temperatura Alta , Humanos , Técnicas In Vitro , Intestino Delgado/microbiologia , Camundongos , Plasmídeos , Coelhos , SorotipagemRESUMO
Enterotoxin was obtained from the culture of E. coli O15 by salt precipitation and gel-chromatography. The toxic activity of the preparation increased during the isolation and purification: 60-fold according to the results of the method of ligated rabbit intestinal segment and 66-100-fold according to the skin test. The "plateau" and the second fraction obtained as a result of gel-chromatography were inactive according to the results of the method of ligated intestinal segment, but possessed PF-activity in the skin test. Two suppositions are put forward: 1) possibly the factor of vascular permeability and the diarrheal factor were two different substances ((molecules), or 2) the skin test was more sensitive for determination of the toxicity than the method of the ligated segment of rabbit intestine.