Effects of leached components from silorane and methacrylate-based dental composites on the male mice reproductive system.

BACKGROUND: This study aimed to investigate the potential toxic effects of leached components from either a methacrylate-based composite (Filtek Z250) or a silorane-based composite (Filtek P90) on the male mice reproductive system. METHODS: Sixty adult Syrian male mice were divided into six groups. In test groups, leached components from composite specimens in artificial saliva or 75% aqueous ethanol solution were administered intragastrically daily for 28 days. The mice were then euthanized and the following reproductive parameters recorded: body weight changes; weight of paired testes; testis volume; Gonadosomatic Index (GSI); sperm motility; sperm viability; daily sperm production and epididymal sperm count. RESULTS: There were no significant differences in body weight changes, weight of paired testes, GSI, testis volume, epididymal sperm count, and daily sperm production between groups. Sperm motility and sperm viability were significantly lower in all the test groups in comparison to the control groups. In addition, they were significantly lower in the test groups in which composite samples were immersed in aqueous ethanol solution. CONCLUSIONS: Within the limitations of this study, the present data indicate that leached components from dental composites could affect sperm quality and therefore could potentially cause adverse effects on the male mice reproductive system.

Evaluation of Bis-GMA/TEGDMA monomers leaching from a hybrid dental composite resin.

AIM: Postcuring elution of unreacted monomers has harmful effects on mechanical characteristics and biocompatibility of resin composite restorations. The purpose of this study was to evaluate the amount of eluted Bis-GMA and TEGDMA monomers from a hybrid dental composite cured with LED or halogen curing unit after immersing in saliva or water. METHODS: Thirty-six composite disc specimens (Hybrid Filtek Z250,3M-ESPE, USA) were made and divided in four groups. After polymerization with LED or halogen curing units, samples were immersed in water or saliva for 24 hours. The amounts of each monomer (Bis-GMA and TEGDMA) released in different mediums were detected by high-performance liquid chromatography (HPLC). Data were analyzed with two-way ANOVA and one-way ANOVA tests. RESULTS: The samples were immersed in water, in both curing methods, released significantly more Bis-GMA than TEGDMA (P<0.05). The total monomers eluted was higher in samples cured with halogen curing unit than LED (P=0.001); in addition, saliva caused elution of more monomer than water (P=0.007). CONCLUSION: In conclusion, chemical property of monomers, power of curing light unit and the type of immersion media play key roles in the quantity of eluted monomers from composite resins. Saliva and halogen curing unit can cause greater monomer leaching from hybrid resins composite.