Superior outcomes after arthroscopic treatment of femoroacetabular impingement and labral tears with closed versus open capsule.

PURPOSE: The purpose of the study was the clinical evaluation of the capsular management with arthroscopic treatment of femoroacetabular impingement and labral tears by comparing the functional outcomes of closed versus open capsule. METHODS: Patients with a median age of 38 years (18-55), clinical and radiological features of FAI and/or labral tear, and non-arthritic non-dysplastic hips were selected for arthroscopic treatment. Capsulotomy was performed primarily as an interportal section, then a distal extension preserving the zona orbicularis was added. The study compared two matched groups: patients with open capsule versus patients with closed capsule. Clinical outcomes were assessed by Non-Arthritic Hip Score, hip outcome scores of daily living activities and sports-specific scales. Scores were collected preoperatively and 6 months, 2 years and 5 years postoperatively. Rate of revision arthroscopy and conversion to total hip arthroplasty were used for comparing groups. Minimal clinically important differences were calculated for both groups. RESULTS: The study included 42 patients in the OC group and 44 patients in the CC group. Significant improvement of postoperative PROMs was recorded in both groups compared to preoperative scores. CC group significantly improved more than the OC group based on NAHS, HOS-ADL and HOS-SSS over all check points except for NAHS and HOS-ADL at 6 months, which were statistically non-significant. A non-significant difference was observed in the percentage of patients who met the MCID for all reported outcome scores at 5 years in both groups. The rate of reoperation was similar in both groups, but with different indications. CONCLUSION: Arthroscopic treatment of FAI and labral repair with complete closure of the hip capsule led to significantly improved functional outcomes after 5 years follow-up compared with open capsule. Closed capsule can provide greater improvement in the sports-specific outcomes at early follow-up. Controlled capsulotomy limited by zona orbicularis did not produce instability at any postoperative stage. Similar proportions of patients achieved minimal clinically important difference, and similar rates of reoperation were reported in both groups. LEVEL OF EVIDENCE: III.

Promising radiological outcome after repair of acetabular chondral defects by microfracture augmented with chitosan-based scaffold: mid-term T2 mapping evaluation.

PURPOSE: Radiological evaluation of the repair tissue produced after arthroscopic treatment of acetabular chondral lesions associated with femoroacetabular impingement (FAI) by the chitosan-based scaffold. METHODS: Patients of age 18-55 years with clinical and radiological features of FAI and non-arthritic non-dysplastic hips were selected for arthroscopic treatment. Full-thickness acetabular chondral defects were filled with chitosan-based scaffold material after microfracture. T2 mapping was carried out for all patients after 24 months using a 1.5-T machine. Nine regions of interest (ROIs) were localized from three consecutive sagittal slices including the area of repair. T2 relaxation times of ROIs in the repair area were compared with the corresponding posterior cartilage. RESULTS: Twenty-one patients, 17 men and 4 women, underwent arthroscopic treatment of full-thickness acetabular chondral defects with mean size of 3.6 ± 1 cm2 (range 2-6 cm2). Zone 2 was affected in all cases while zone 3 was involved in 13 cases. T2 relaxation values were collected from 189 ROIs for quantitative analysis. Within the peripheral repair area, the mean T2 value was 49.1 ± 7.2 ms (ms), while ROIs of the central repair area had mean T2 values of 50.2 ± 7.1 ms. Posterior cartilage showed mean T2 value of 46.2 ± 7.6 ms CONCLUSION: Arthroscopic microfracture of large full-thickness acetabular chondral defects with chitosan-based scaffold produced a homogenous repair tissue similar to the corresponding native cartilage of the same joint on quantitative T2 mapping at mid-term follow-up. CLINICAL RELEVANCE: augmentation of the microfracture by chitosan-based scaffold is a promising modality for treatment of large full-thickness acetabular defects. LEVEL OF EVIDENCE: IV.

Arthroscopic Repair of Acetabular Cartilage Lesions by Chitosan-Based Scaffold: Clinical Evaluation at Minimum 2 Years Follow-up.

PURPOSE: To evaluate the functional outcome of using chitosan-based material in our patients after 2 years of follow-up. METHODS: Nonarthritic nondysplastic femoroacetabular impingement patients with an acetabular chondral lesion, 18 to 55 years of age, were included for arthroscopic repair between May 2013 and July 2015. Full-thickness chondral defects ≥2 cm2 were filled with chitosan-based implant after microfractures. Follow-up consisted of alpha angle assessment and clinical outcome in the form of the Non Arthritic Hip Score (NAHS), International Hip Outcome Tool 33 (iHOT33), Hip Outcome Score of Activities of Daily Living (HOS-ADL), and Hip Outcome Score of Sports Specific Scale (HOS-SSS). RESULTS: Twenty-three patients were included. The mean follow-up was 38.4 ± 7.0 months (range, 24-50 months). The mean defect size was 3.5 ± 1.0 cm2, principally involving zone 2 and to a lesser extent in zones 1 and 3. Using femoroplasty, the alpha angle was corrected from a mean 70.5 ± 6.3° to 44.3 ± 4.9° (P = .00001). Significant improvement occurred comparing the preoperative to the first-year postoperative patient-reported outcomes: P = .00001 for the NAHS, P = .00004 for the iHOT33, P = .00005 for the HOS-ADL, and P = .0002 for the HOS-SSS. No statistically significant change has been observed in the patient-reported outcomes obtained at the endpoint when compared with the first-year values (P = .13 for the NAHS, P = .21 for the HOS-ADL, and P = .29 for the HOS-SSS), except for the iHOT33, which showed further significant improvement (P = .02). Up to 91% of the patients met or exceeded the minimal clinically important difference. One patient needed total hip arthroplasty. Perineal hypoesthesia occurred in 3 patients, who recovered within 2 to 6 weeks, and 1 patient needed a prolonged physiotherapy program for postoperative muscular stiffness. CONCLUSIONS: The arthroscopic combined treatment of microfractures and chitosan-based scaffold has maintained satisfactory clinical outcomes in 91% of the patients with s large (≥2 cm2) full-thickness acetabular chondral defect associated with femoroacetabular impingement at a mean follow-up of 38.4 months. The study could not definitely draw any conclusion regarding the safety of chitosan-based material for use in the hip joint. LEVEL OF EVIDENCE: Level IV, case series.

Results of arthroscopic treatment of chondral delamination in femoroacetabular impingement with bone marrow stimulation and BST-CarGel®.

OBJECTIVES: The purpose of this study is to show the preliminary results of using chitosan-based scaffold (BST-CarGel®) with microfracture for treatment of acetabular chondral delamination associated with femoroacetabular impingement. METHODS: A prospective study was performed on 13 hips. Patients were selected in the age group between 18 and 50 years. Patients with delamination of acetabular cartilage associated with femoroacetabular impingement received arthroscopic debridement and microfracture technique. Then cases with defect > 2 cm2 were considered for the application of BST-CarGel® and included in the study. Also, reattachment of the torn labrum and resection of the cam deformity were performed according to the case. For evaluation of the functional outcome, the patients had completed the hip outcome score (HOS) pre- and post-operatively. For evaluation of the regeneration of the cartilage, delayed gadolinium-enhanced MRI of cartilage (dGEMRIC) was used and the percentage of defect filling and type of cartilage studied. RESULTS: Patients had a mean age of 41 years, with moderate to high level of activity (mean Tegner scale 7). The mean size of the chondral defect after debridement was 3.7 cm2. The mean HOS for daily live activities has been improved from 64.4 to 87.4 and for sports subscale from 35.2 to 75.2, which is statistically highly significant. All patients had > 90% of filling of chondral defect. CONCLUSION: The use of BST-CarGel® with microfracture for treatment of acetabular chondral delamination associated with femoroacetabular impingement can improve the functional outcome at two years, with a complete restoration of the cartilage defect in magnetic resonance images (MRI) with specific cartilage sequences.

The expression of propionicin PLG-1 gene (plg-1) by lactic starters.

Propionicin PLG-1 is a bacteriocin produced by Propionibacterium thoenii P127. Such bacteriocin inhibits wide range of food-borne pathogens such as pathogenic Escherichia coli, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Yersinia enterocolitica and a strain of Corynebacterium sp. In the present study, plg-1 gene expressing propionicin PLG-1 was isolated, sequenced for the first time and the resulting sequence was analysed using several web-based bioinformatics programs. The PCR product containing plg-1 gene was transferred to different lactic acid bacterial (LAB) strains using pLEB590 as a cloning vector to give the modified vector pLEBPLG-1. LAB transformants showed an antimicrobial activity against Esch. coli DH5α (most affected strain), Listeria monocytogenes 18116, and Salmonella enterica 25566 as model pathogenic strains. Such LAB transformants can be used in dairy industry to control the food-borne pathogens that are largely distributed worldwide and to feed schoolchildren in the poor countries where dangerous epidemic diseases and diarrhoea prevail.