RESUMO
BACKGROUND: Mxi1, an antagonist of c-Myc, maps to human chromosome 10q24-q25, a region altered in a substantial fraction of prostate tumors. Mice deficient for Mxi1 exhibit significant prostate hyperplasia. We studied the ability of Mxi1 to act as a growth suppressor in prostate tumor cells. METHODS: We infected DU145 prostate carcinoma cells with an Mxi1-expressing adenovirus (AdMxi1) in vitro, and measured Mxi1 expression, cell proliferation, soft agar colony formation, and cell cycle distribution. To explore mechanisms of Mxi1-induced growth arrest, we performed gene expression analysis. RESULTS: AdMxi1 infection resulted in reduced cell proliferation, reduced soft agar colony formation, and a higher proportion of cells in the G(2)/M phase of the cell cycle. This G(2)/M growth arrest was associated with elevated levels of cyclin B, and reduced levels of c-MYC and MDM2. CONCLUSIONS: The ability of AdMxi1 to suppress prostate tumor cell proliferation supports a role for Mxi1 loss in the pathogenesis of a subset of human prostate cancers. Prostate 47:194-204, 2001.
Assuntos
Proteínas de Ligação a DNA/fisiologia , Neoplasias da Próstata/patologia , Fatores de Transcrição/fisiologia , Adenoviridae/genética , Fatores de Transcrição Hélice-Alça-Hélice Básicos , Western Blotting , Divisão Celular/fisiologia , Proteínas de Ligação a DNA/biossíntese , Proteínas de Ligação a DNA/genética , Citometria de Fluxo , Fase G2/fisiologia , Perfilação da Expressão Gênica , Humanos , Masculino , Microscopia Confocal , Mitose/fisiologia , Neoplasias da Próstata/genética , Neoplasias da Próstata/metabolismo , Proteínas Proto-Oncogênicas c-myc/antagonistas & inibidores , Fatores de Transcrição/biossíntese , Fatores de Transcrição/genética , Células Tumorais Cultivadas , Proteínas Supressoras de TumorRESUMO
Pediatric hematology and oncology has only recently started developing within Pakistan, with centers in Islamabad, Lahore, Karachi, and Multan. Efforts are being made to update blood bank facilities and to standardize the level of care being provided throughout the country. A pediatric oncology group has recently been established. A lot of work still needs to be done, however.
Assuntos
Hematologia/tendências , Oncologia/tendências , Pediatria/tendências , PaquistãoRESUMO
In 52 children with cancer, the effect of nutritional status on infection rate was studied. Thirty-two children had solid tumors and 20 had leukemia. Their treatment courses were arbitrarily divided into 3-month periods. Where treatment lasted for 9 months or more, the initial Nutritional Status score was correlated with infection rate during the subsequent 3-month period. Among the leukemic patients, a statistically significant inverse correlation (P < 0.05) was found between nutritional status and ensuing infection rate. No such correlation was found in children with solid tumors. Based on these findings, we recommend nutritional support to children with leukemia during chemotherapy to attempt to reduce the infection rate.