RESUMO
Coenzyme Q (CoQ) is essential for mitochondrial respiration and as a cofactor for sulfide quinone reductase. Schizosaccharomyces pombe produces a human-type CoQ10. Here, we analyzed CoQ in other fission yeast species. S. cryophilus and S. octosporus produce CoQ9. S. japonicus produces low levels of CoQ10, although all necessary genes for CoQ synthesis have been identified in its genome. We expressed three genes (dps1, dlp1, and ppt1) for CoQ synthesis from S. japonicus in the corresponding S. pombe mutants, and confirmed that they were functional. S. japonicus had very low levels of oxygen consumption and was essentially respiration defective, probably due to mitochondrial dysfunction. S. japonicus grows well on minimal medium during anaerobic culture, indicating that it acquires sufficient energy by fermentation. S. japonicus produces comparable levels of ethanol under both normal and elevated temperature (42 °C) conditions, at which S. pombe is not able to grow.
Assuntos
Etanol/metabolismo , Consumo de Oxigênio , Schizosaccharomyces/metabolismo , Ubiquinona/análogos & derivados , Cromatografia Líquida , Genes Fúngicos , Espectrometria de Massas , Estresse Oxidativo , Schizosaccharomyces/genética , Ubiquinona/biossínteseRESUMO
Three natural hybrids and an introgressed individual of Ligularia were evaluated based on a combination of morphology, root chemicals, and nucleotide sequences of evolutionally neutral regions to understand the chemical outcomes of hybridization and introgression. Six previously undescribed eremophilane sesquiterpenes were isolated from hybrids between L. cyathiceps and L. lamarum/L. subspicata, and benzofurans were isolated from L. subspicata for the first time. Their structures were elucidated based on spectroscopic analyses. Some compounds produced by hybrids have not been detected in either parental species, indicating that the metabolic profile was altered by hybridization and introgression.
Assuntos
Asteraceae/química , Benzofuranos/química , Hibridização Genética , Sesquiterpenos/química , Asteraceae/genética , Sequência de Bases , Benzofuranos/isolamento & purificação , China , DNA de Plantas/genética , Metaboloma , Estrutura Molecular , Raízes de Plantas/química , Sesquiterpenos/isolamento & purificaçãoRESUMO
Three new eremophilanes were isolated from a sample of Ligularia collected in Yunnan province of China and their structures were determined. The chemical - constituents including eleven eremophilanes (major constituents: 6-acyloxyfuranoeremophilan-IOß-ols) were similar to those of a hybrid of L. cyathiceps and L. subspicata, however, the base sequence of the ITS 1-5.8S-ITS2 region was typical of L. cyathiceps, indicating backcrossing.