RESUMO
The fruitfly, Drosophila melanogaster, has three proPO genes (DoxA1, CG8193 and CG2952). DoxA1 has been shown to encode proPO A(1), one of the two proPO isoforms (A(1) and A(3)). However, which of CG8193 or CG2952 encodes proPO A(3) has so far remained elusive. In Northern analysis, CG8193 expression was strong during the larval stage, yet expression of CG2952 was not detected at any stage. Immunoblot analyses with specific antibodies detected CG8193 in the larval hemolymph at the mobility of the endogenous proPOA(3), though no signal for CG2952. These results indicate that the expression of CG2952 is very low and that CG8193 is the gene that encodes proPO A3. Processing of A(1)and A(3) isoforms in adult homogenate and activity of recombinant proPOs were also investigated.
Assuntos
Catecol Oxidase/genética , Drosophila melanogaster/enzimologia , Drosophila melanogaster/genética , Precursores Enzimáticos/genética , Genes de Insetos , Animais , Especificidade de Anticorpos , Catecol Oxidase/sangue , Catecol Oxidase/imunologia , Eletroforese em Gel Bidimensional , Precursores Enzimáticos/sangue , Precursores Enzimáticos/imunologia , Regulação da Expressão Gênica no Desenvolvimento , Regulação Enzimológica da Expressão Gênica , Immunoblotting , Isoenzimas/sangue , Isoenzimas/genética , Isoenzimas/imunologia , Larva/enzimologia , Processamento de Proteína Pós-Traducional , Proteínas Recombinantes/metabolismoAssuntos
Recidiva Local de Neoplasia , Neoplasias Gástricas , Biomarcadores Tumorais/sangue , Humanos , Neoplasias Hepáticas/secundário , Metástase Linfática , Imageamento por Ressonância Magnética , Recidiva Local de Neoplasia/diagnóstico , Recidiva Local de Neoplasia/patologia , Neoplasias Gástricas/diagnóstico , Neoplasias Gástricas/patologia , Tomografia Computadorizada por Raios X , UltrassonografiaAssuntos
Terapia por Infusões no Domicílio , Neoplasias Gástricas/tratamento farmacológico , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Camptotecina/administração & dosagem , Camptotecina/análogos & derivados , Cisplatino/administração & dosagem , Esquema de Medicação , Determinação de Ponto Final , Fluoruracila/administração & dosagem , Humanos , Irinotecano , Qualidade de VidaRESUMO
Recently, the progress in chemotherapy has brought an improvement in the survival rate of patients with non-resectable digestive tract cancer. Simultaneously, maintenance of patients' QOL is regarded as important. In our hospital, we use low-dose FP therapy to maintain QOL and a satisfactory survival rate. To date, we have administered low-dose FP to more than 50 patients with non-resectable digestive cancer. We investigated patients who received home chemotherapy after being evaluated NC, and found no remarkable side effects as a result of this therapy during admission.
Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Comportamento Cooperativo , Serviços Hospitalares de Assistência Domiciliar , Neoplasias/tratamento farmacológico , Cisplatino/administração & dosagem , Esquema de Medicação , Fluoruracila/administração & dosagem , Humanos , Qualidade de Vida , Tegafur/administração & dosagem , Uracila/administração & dosagemRESUMO
In our G.I. department, patients with inoperable or recurrent cancer of the digestive organs are treated by chemotherapy and/or radiation therapy. Since the efficacy of these modalities has been poor in our hospital as in others, we have attempted to maintain good quality of life (QOL) in these patients by offering hospice care in the hospital or at home. Home care is provided to those who choose not to have chemotherapy or do not respond well to it. These patients live within 20 km of the hospital and are cared for at home by a visiting nurse or through visits to our outpatient clinic. However, in the latter patients who do not have access to good public transportation, clinic visits are a hardship to both patients and their families even though they do well with home hospice care. Home hospice care is also difficult for those who do not have adequate family support available. We report here three patients with terminal gastric cancer who appeared to experience good QOL through home hospice care.
