How to transform urban institutional green spaces into Ancillary Botanic Gardens to expand informal botanical learning opportunities in cities.

Since many cities lack botanical gardens, we introduced the concept of Ancillary Botanic Gardens (ABG), which builds on the premise that organizations can expand informal botanical learning by adding a secondary function to their institutional green spaces. This study guides the application of the ABG concept in various spatial and functional contexts by offering practical and interpretive tools to organizations who are less used to working with nature but are interested in mitigating urban residents' detachment from nature. Online maps of 220 botanic gardens were reviewed to define types of plant collections and produce an exhaustive list of physical botanic garden elements. The collected information was developed into an ABG field checklist that was tested on three case studies in Lebanon and then used to develop guidelines for ABG establishment. The guidelines and checklist are meant to empower and guide organizations interested in establishing an ABG.

Biodiversity conservation in cities: Defining habitat analogues for plant species of conservation interest.

SYNTHESIS AND APPLICATIONS: The stepwise method was useful in producing informative plant lists and assemblages for planting designs and landscape management; it generated a plant selection palette that is not restrictive and does not enforce a native only policy. It also offered a wide range of potential habitat analogues for M. crassifolia.

Autographa californica M nuclear polyhedrosis virus and cytochalasin D: antagonists in the regulation of protein synthesis.

During infection by the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV), synthesis of host proteins in IPLB-Sf-21 cells is inhibited. We report here that cytochalasin D (CD), a drug that specifically interacts with actin, behaved as an antagonist in the virus-mediated shutdown of host proteins actin and tubulin. In uninfected cells, CD caused an increase in actin synthesis but had no apparent effect on tubulin synthesis. In infected cells, CD similarly enhanced actin synthesis early in infection and delayed the virus shutoff of actin synthesis by 14 hr. The shutoff of tubulin synthesis was delayed by 8 hr. Addition of CD to infected cells after host protein synthesis ceased resulted in an induction of actin synthesis reversing viral inhibitory effects. Similarly, the removal of CD resulted in virus-induced inhibition of actin synthesis. Treatment of infected cells with CD caused a delay in the onset and/or shutoff of at least five viral proteins and inhibited the amplification of polyhedrin synthesis by at least 8 hr.