Efectos del parto y ooforectomía sobre el colágeno uretral: estudio experimental / Effects of Delivery and Oophorectomy on Urethral Collagen: An Experimental Study

Objetivo: Realizar una evaluación estereológica de la concentración de fibras colágenas tipo I y III en el tejido uretral de ratas sometidas a simulación de trabajo de parto y ooforectomía. Comparar las concentraciones de colágeno entre ratas ooforectomizadas y no ooforectomizadas. Material y método: Sesenta ratas Wistar adultas se dividieron en 6 grupos. Como control se utilizó un grupo compuesto por ratas vírgenes y otro por ratas ooforectomizadas. Dos grupos se sometieron a distensión vaginal durante 30 y 120 minutos, respectivamente. Los otros dos grupos se sometieron a iguales períodos de distensión, seguidos de ooforectomía. Sesenta días después se les realizó eutanasia y extracción de tejido uretral para el análisis estereológico de las fibras colágenas tipo I y III tras tinción con hematoxilina-eosina y rojo picrosirius. Resultados: Las ratas ooforectomizadas mostraron una disminución en los niveles estrogénicos. Hubo reducción de colágeno tipo III en el grupo control ooforectomizado respecto al control al analizarse de manera independiente, sin evidenciarse diferencias significativas entre los demás grupos. El colágeno tipo I disminuyó en todos los grupos con respecto al control, pero en el grupo de distensión vaginal prolongada y ooforectomía estas fibras aumentaron. Conclusión: En ratas normoestrogénicas la simulación del trabajo de parto no altera los niveles de colágeno III. En ratas hipoestrogénicas disminuyó la concentración de colágeno tipo I y III, pero al ser sometidas a trabajo de parto prolongado el colágeno I aumentó (AU)

Objective: Stereological evaluation of the concentration of type I and III collagen fibers in the urethral tissue of rats subjected to simulated labor and oophorectomy. To compare the concentrations of collagen between oophorectomized and non-oophorectomized rats. Material and method: Sixty adult Wistar rats were divided into six groups. A group made up of virgin rats was used as control group and another group was made up of oophorectomized rats. Two groups underwent vaginal distention for 30 and 120minutes, respectively. The two other groups were subjected to the same distension periods, followed by oophorectomy. Sixty days later, euthanasia and removal of urethral tissue was carried out for stereological analysis of type I and III collagen after staining with hematoxylin and eosin and picrosirius red. Results: A decrease in estrogen levels was observed in the oophorectomized rats. There was a reduction of type III collagen in the oophorectomized control group compared to the control group when analyzed independently. No significant differences were observed among the other groups. Type I collagen decreased in all groups compared to the control group. However, in the prolonged vaginal distension and oophorectomy group, these fibers increased. Conclusion: In normal rats, simulation of labor does not alter the collagen III levels. In hypoestrogenic rats, the concentration of collagen type I and III decreased, except in those undergoing prolonged labor simulation in which collagen I increased (AU)

[Effects of delivery and oophorectomy on urethral collagen: an experimental study]. / Efectos del parto y ooforectomía sobre el colágeno uretral: estudio experimental.

OBJECTIVE: Stereological evaluation of the concentration of type I and III collagen fibers in the urethral tissue of rats subjected to simulated labor and oophorectomy. To compare the concentrations of collagen between oophorectomized and non-oophorectomized rats. MATERIAL AND METHOD: Sixty adult Wistar rats were divided into six groups. A group made up of virgin rats was used as control group and another group was made up of oophorectomized rats. Two groups underwent vaginal distention for 30 and 120 minutes, respectively. The two other groups were subjected to the same distension periods, followed by oophorectomy. Sixty days later, euthanasia and removal of urethral tissue was carried out for stereological analysis of type I and III collagen after staining with hematoxylin and eosin and picrosirius red. RESULTS: A decrease in estrogen levels was observed in the oophorectomized rats. There was a reduction of type III collagen in the oophorectomized control group compared to the control group when analyzed independently. No significant differences were observed among the other groups. Type I collagen decreased in all groups compared to the control group. However, in the prolonged vaginal distension and oophorectomy group, these fibers increased. CONCLUSION: In normal rats, simulation of labor does not alter the collagen III levels. In hypoestrogenic rats, the concentration of collagen type I and III decreased, except in those undergoing prolonged labor simulation in which collagen I increased.

Alteracoes angiograficas do tumor de bacinete. / Angiographic changes of tumors of the kidney pelvis

Sao mostrados tres casos de neoplasias uroteliais de localizacao pielica em que a urografia excretora se mostrou inconclusiva, lancando-se mao dos recursos angiograficos para melhor elucidacao diagnostica.Apresentam-se as principais alteracoes angiograficas encontradas, enfatizando-se a importancia desse metodo diagnostico quando a urografia excretora e a pielografia ascendente sao insatisfatorias