Mycobacterium tuberculosis/Mycobacterium bovis triggered different variations in lipid composition of Bovine Alveolar Macrophages.

The lipid composition performs important functions in interaction between macropha-ge and Mycobacterium tuberculosis (MTB)/Mycobacterium bovis (MB). Current understanding regarding the lipid responses of bovine alveolar macrophage (BAM) to MTB/MB is quite limited. The present study conducted lipidomics and transcriptome to assess alterations in BAM lipid compositions upon MB and MTB infection. We found that both MTB and MB induced glycerophospholipids accumulation in BAM, and MTB induced more alterations in lipid composition. MTB could affect the contents of various lipids, especially ceramide phosphocholines, polystyrene (PS) (17:0/0:0), testolic acid and testosterone acetate. Meanwhile, MB particularly induced accumulation of 1-alkyl,2-acylglycerophosphoinositols. Both MB and MTB suppressed the contents of palmitoleamide, N-ethyl arachidonoyl amine, N-(1,1-dimethyl-2-hydroxy-ethyl) arachidonoyll amine, eicosanoyl-EA, and PS (O-18:0/17:0) in BAM. Additionally, transcriptome analysis revealed that only MTB triggered genes involved in immune signaling and lipid related pathways in BAM. And MTB mainly activated genes CXCL2 and CXCL3 relevant to NOD-like receptor, IL-17 and TNF to further induce lipid accumulation in BAM, which in turn promoted the formation of foam cells. Meanwhile, time course RT-qPCR results showed that MTB was recognized by BAM to triggered dramatic immune responses, whereas MB could effectively escape the recognition system of BAM, leading rearrangement of lipid metabolisms in BAM at early infection stage. Altogether, the results of the present study provided evidence for changes in lipid metabolism of MTB/MB attacked BAM and contributed to the detection and treatment of zoonotic tuberculosis.

Functions of 1,25-dihydroxy vitamin D3, vitamin D3 receptor and interleukin-22 involved in pathogenesis of gout arthritis through altering metabolic pattern and inflammatory responses.

BACKGROUND: Gouty arthritis (GA) is a common type of inflammatory arthritis. Recent studies demonstrated that 1,25-dihydroxy vitamin D3 (1,25(OH) 2 VD3) and vitamin D3 receptor (VD-R) play a protective role in acute inflammation, but interleukin-22(IL-22) promotes inflammation, especially for arthritis. However, our understanding of the responses of 1,25(OH) 2VD3 and IL-22 to gout was still unclear. Presently, in-depth metabolomics, bioinformatics and clinical characteristics analyses were performed to elucidate the pathogenesis and valuable clinical indicators of gouty arthritis. METHODS: Peripheral venous blood was taken for investigation. The levels of IL-22 and 1,25(OH)2VD3 were determined in patient's plasma via ELISA, and the mRNA levels of IL-22 and VD-R were measured via qRT-PCR. The interaction network of VD-R and IL22 were constructed by the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING), and the biological function of the related proteins were analyzed by Clusterprofiler Metabolomics were performed to decipher the metabolic variations of GA. RESULTS: The levels of VD-R and 1,25(OH) 2 VD3 were identified to be low. What,s more, GA patients were reported to have high expression of IL-22. And IL-22 levels positively correlated with C-reactiveprotein (CRP) serum levels in the bivariate correlation analysis, whereas the level of 1,25(OH) 2VD3 negatively correlated with that of CRP. GO and KEGG analyses revealed that IL-22 and 1,25(OH) 2 VD3 were involved in stress immunity and inflammatory responses. These pathways are known to play a role in GA pathogenesis. Meanwhile, the metabolic profiles of GA serum showed that the increase in various amino acids and uric acid are involved in GA pathogenesis. Importantly, VD-R and IL22 closely correlated with the level of key metabolites uric acid, whose increase promoted the occurrence of GA. CONCLUSION: GA patients have low levels of VD-R and 1,25(OH) 2 VD3, and high levels of IL-22 together with various amino acids and uric acid. The levels of IL-22 and 1,25(OH) 2VD3 were positively and negatively correlated with C-reactive protein (CRP) serum levels, respectively. Both IL-22 and 1,25(OH) 2 VD3 functioned in GA-related immune and inflammatory responses, and closely correlated with the level of GA-related uric acid. Overall, IL-22, VD-R and 1,25(OH) 2 VD3 play functionally important roles in inflammatory responses and are relevant to gout pathogenesis.

Tannic acid/CaII anchored on the surface of chitin nanofiber sponge by layer-by-layer deposition: Integrating effective antibacterial and hemostatic performance.

Massive blood loss and bacterial infection are major challenges for global public health. However, traditional wound dressings cannot fully meet the current clinical needs in controlling bleeding and avoiding infection. In this study, we prepared a chitin nanofiber suspension by green mechanical grinding and homogenizing, which could be used for developing a porous chitin nanofiber sponge by freeze drying. Then tannic acid/CaII was anchored on the surface of chitin nanofiber sponge by layer-by-layer deposition based on the coordination complex of tannic acid and Ca2+. These porous chitin nanofiber sponges with meso-macroporous structure could accelerate platelet aggregation for hemostasis. Hemostasis tests also demonstrated that the Ca2+ and TA in the TA/CaII coating on chitin nanofiber sponge could accelerate platelet activity and shorten the hemostatic time both in vitro and in vivo. Besides, the TA in the TA/CaII coating enhanced the antibacterial properties of the sponge, which depended the content of TA/CaII. Based on these results, it could be inferred that the chitin nanofiber sponges anchored TA/CaII coating had rapid hemostatic and antibacterial properties, which would have great potential for designing hemostatic product in clinical application.

Near-Infrared Light-Steered Graphene Aerogel Micromotor with High Speed and Precise Navigation for Active Transport and Microassembly.

Fuel-free light-driven micromotors have attracted increasing attention since the advantages of reversible, noninvasive, and remote maneuver are on demand with excellent spatial and temporal resolution. However, they suffer from a challenging bottleneck of the rather modest motion speed, which hinders their applications, needing to overcome the water flow movement in environmental water. Herein, we demonstrate a near-infrared (NIR) light-steered, precise navigation-controlled micromotor based on a reduced graphene oxide aerogel microsphere (RGOAM), which possesses an isotropic structure and is easily prepared by a one-step electrospray approach other than conventional light-propelled micromotors with the Janus structure. Benefiting from the ultralight weight of the aerogel and lesser fluid resistance on the water surface, the RGOAM motors show a higher motion speed (up to 17.60 mm/s) than that in the published literature, letting it overcome counterflow. Taking advantage of the photothermal conversion capacity of the RGOAM under an asymmetric light field, it is capable of moving both on the water driven by the Marangoni effect and under the water via light-manipulated density change. The motion direction and speed on water as well as the "start/stop" state can be precisely steered by NIR light even in a complicated maze. Due to its strong adsorption and loading capacity, the RGOAM can be applied for active loading-transport-release of dyes on demand as well as micropart assembling and shaping. Our work provides a strategy to achieve high speed, precise navigation control, and functional extensibility simultaneously for micromotors, which may offer considerable promise for the broad biomedical and environmental applications.