RESUMO
There have been extensive observations that RNA containing repetitive elements accumulates in transformed cells and tumor tissues. In the present study, we first obtained result consistent with previous observations by in situ hybridization. Then we used primer extension analysis to determine the level of polymerase III directed Alu RNA and found an increased expression of Alu RNA in hepatocellular carcinoma.
Assuntos
Elementos Alu , Carcinoma Hepatocelular/enzimologia , Carcinoma Hepatocelular/genética , Neoplasias Hepáticas/enzimologia , Neoplasias Hepáticas/genética , RNA Polimerase III/fisiologia , Transcrição Gênica , Primers do DNA/química , Humanos , Hibridização In Situ , Dados de Sequência Molecular , RNA/metabolismo , RNA Polimerase III/metabolismo , RNA Mensageiro/metabolismoRESUMO
BACKGROUND: The aim of this study was to investigate the association between thunderstorms and mortality of in-patients. METHODS: Data for in-patients and deaths in internal medicine departments from four hospitals in Shanghai were collected and grouped according to meteorological conditions. RESULTS: Weekly mortality of in-patients was significantly higher on days with thunderstorms than on fine days. This effect was significant for patients in respiratory, cardiovascular and neurology wards but not in the wards of other specialties.
Assuntos
Mortalidade Hospitalar/tendências , Raio , Chuva , Distribuição de Qui-Quadrado , China/epidemiologia , Humanos , Projetos Piloto , Fatores de RiscoRESUMO
BACKGROUND: Regarding diagnostic reasoning, the currently taught Bayesian theory is a form of hypothetical-deduction reasoning. Using set theory, we offer syllogism reasoning instead of hypothetical-deductive reasoning and establish an online diagnostic expert system model based on this diagnostic methodology. METHODS: Concepts of set theory were employed to demonstrate diagnostic reasoning. ASP, Vbscript and Microsoft Access were used to establish the expert system and data of 50 cases from Shanghai pulmonary hospital were put into the program to test its efficiency. CONCLUSION: Diagnostic procedure is type of syllogism rather than hypothetical-deductive reasoning.
