Practical Guide to Measuring Wetland Carbon Pools and Fluxes.

Wetlands cover a small portion of the world, but have disproportionate influence on global carbon (C) sequestration, carbon dioxide and methane emissions, and aquatic C fluxes. However, the underlying biogeochemical processes that affect wetland C pools and fluxes are complex and dynamic, making measurements of wetland C challenging. Over decades of research, many observational, experimental, and analytical approaches have been developed to understand and quantify pools and fluxes of wetland C. Sampling approaches range in their representation of wetland C from short to long timeframes and local to landscape spatial scales. This review summarizes common and cutting-edge methodological approaches for quantifying wetland C pools and fluxes. We first define each of the major C pools and fluxes and provide rationale for their importance to wetland C dynamics. For each approach, we clarify what component of wetland C is measured and its spatial and temporal representativeness and constraints. We describe practical considerations for each approach, such as where and when an approach is typically used, who can conduct the measurements (expertise, training requirements), and how approaches are conducted, including considerations on equipment complexity and costs. Finally, we review key covariates and ancillary measurements that enhance the interpretation of findings and facilitate model development. The protocols that we describe to measure soil, water, vegetation, and gases are also relevant for related disciplines such as ecology. Improved quality and consistency of data collection and reporting across studies will help reduce global uncertainties and develop management strategies to use wetlands as nature-based climate solutions. Supplementary Information: The online version contains supplementary material available at 10.1007/s13157-023-01722-2.

Large increases in methane emissions expected from North America's largest wetland complex.

Natural methane (CH4) emissions from aquatic ecosystems may rise because of human-induced climate warming, although the magnitude of increase is highly uncertain. Using an exceptionally large CH4 flux dataset (~19,000 chamber measurements) and remotely sensed information, we modeled plot- and landscape-scale wetland CH4 emissions from the Prairie Pothole Region (PPR), North America's largest wetland complex. Plot-scale CH4 emissions were driven by hydrology, temperature, vegetation, and wetland size. Historically, landscape-scale PPR wetland CH4 emissions were largely dependent on total wetland extent. However, regardless of future wetland extent, PPR CH4 emissions are predicted to increase by two- or threefold by 2100 under moderate or severe warming scenarios, respectively. Our findings suggest that international efforts to decrease atmospheric CH4 concentrations should jointly account for anthropogenic and natural emissions to maintain climate mitigation targets to the end of the century.

Using a vegetation index to assess wetland condition in the Prairie Pothole Region of North America.

Wetlands deliver a suite of ecosystem services to society. Anthropogenic activities, such as wetland drainage, have resulted in considerable wetland loss and degradation, diminishing the intrinsic value of wetland ecosystems worldwide. Protecting remaining wetlands and restoring degraded wetlands are common management practices to preserve and reclaim wetland benefits to society. Accordingly, methods for monitoring and assessing wetlands are required to evaluate their ecologic condition and outcomes of restoration activities. We used an established methodology for conducting vegetation-based assessments and describe a case study consisting of a wetland condition assessment in the Prairie Pothole Region of the North American Great Plains. We provide an overview of an existing method for selecting wetlands to sample across broad geographic distributions using a spatially balanced statistical design. We also describe site assessment protocols, including vegetation survey methods, and how field data were applied to a vegetation index that categorized wetlands according to ecologic condition. Results of the case study indicated that vegetation communities in nearly 50% of the surveyed wetlands were in very poor or poor condition, while only about 25% were considered good or very good. Approximately 70% of wetlands in native grasslands were categorized as good or very good compared to only 12% of those in reseeded grasslands (formerly cropland). In terms of informing restoration and management activities, results indicated that improved restoration practices could include a greater focus on establishing natural vegetation communities, and both restored and native prairie wetlands would benefit from enhanced management of invasive species.

Soil organic carbon stocks and sequestration rates of inland, freshwater wetlands: Sources of variability and uncertainty.

Impacts of land use, specifically soil disturbance, are linked to reductions of soil organic carbon (SOC) stocks. Correspondingly, ecosystem restoration is promoted to sequester SOC to mitigate anthropogenic greenhouse gas emissions, which are exacerbating global climate change. Restored wetlands have relatively high potential to sequester carbon compared to other ecosystems, but SOC accumulation rates are variable, which leads to high uncertainty in sequestration rates. To assess soil properties and carbon sequestration rates of freshwater mineral soil wetlands, we analyzed an extensive database of SOC concentrations from the Prairie Pothole Region (549 wetlands over 160,000 km2), which is considered one of the largest wetland ecosystems in North America. We demonstrate that SOC of wetland catchments varies among inner, transition, toe slope, and upland landscape positions (LSPs), as well as among land uses and soil depth segments. Soil organic carbon concentrations were greatest in the inner portion of the catchment (66 Mg ha-1) and progressively decrease towards the upland LSP (43 Mg ha-1). We also conducted a regional extrapolation based on LSP- and land-use-specific SOC stocks, and estimated that wetland and upland areas of PPR wetland catchments contain 141 and 178 Tg of SOC in the upper 15 cm of the soil profile, respectively. Regressing SOC by restoration age (years restored) showed that sequestration rates, which differ by LSP and depth, ranged from 0.35 to 1.10 Mg ha-1 year-1. Using these SOC sequestration rates, along with data from natural and cropland reference sites, we estimated that it takes 20 to 64 years for SOC levels of restored wetlands to return to natural reference conditions, depending on LSP and depth segment. Accounting for LSP reduces uncertainty and should refine future assessments of the greenhouse gas mitigation potential from wetland restoration.

Abundant carbon substrates drive extremely high sulfate reduction rates and methane fluxes in Prairie Pothole Wetlands.

Inland waters are increasingly recognized as critical sites of methane emissions to the atmosphere, but the biogeochemical reactions driving such fluxes are less well understood. The Prairie Pothole Region (PPR) of North America is one of the largest wetland complexes in the world, containing millions of small, shallow wetlands. The sediment pore waters of PPR wetlands contain some of the highest concentrations of dissolved organic carbon (DOC) and sulfur species ever recorded in terrestrial aquatic environments. Using a suite of geochemical and microbiological analyses, we measured the impact of sedimentary carbon and sulfur transformations in these wetlands on methane fluxes to the atmosphere. This research represents the first study of coupled geochemistry and microbiology within the PPR and demonstrates how the conversion of abundant labile DOC pools into methane results in some of the highest fluxes of this greenhouse gas to the atmosphere ever reported. Abundant DOC and sulfate additionally supported some of the highest sulfate reduction rates ever measured in terrestrial aquatic environments, which we infer to account for a large fraction of carbon mineralization in this system. Methane accumulations in zones of active sulfate reduction may be due to either the transport of free methane gas from deeper locations or the co-occurrence of methanogenesis and sulfate reduction. If both respiratory processes are concurrent, any competitive inhibition of methanogenesis by sulfate-reducing bacteria may be lessened by the presence of large labile DOC pools that yield noncompetitive substrates such as methanol. Our results reveal some of the underlying mechanisms that make PPR wetlands biogeochemical hotspots, which ultimately leads to their critical, but poorly recognized role in regional greenhouse gas emissions.

Land use effects on pesticides in sediments of prairie pothole wetlands in North and South Dakota.

Prairie potholes are the dominant wetland type in the intensively cultivated northern Great Plains of North America, and thus have the potential to receive pesticide runoff and drift. We examined the presence of pesticides in sediments of 151 wetlands split among the three dominant land use types, Conservation Reserve Program (CRP), cropland, and native prairie, in North and South Dakota in 2011. Herbicides (glyphosate and atrazine) and fungicides were detected regularly, with no insecticide detections. Glyphosate was the most detected pesticide, occurring in 61% of all wetlands, with atrazine in only 8% of wetlands. Pyraclostrobin was one of five fungicides detected, but the only one of significance, being detected in 31% of wetlands. Glyphosate was the only pesticide that differed by land use, with concentrations in cropland over four-times that in either native prairie or CRP, which were equal in concentration and frequency of detection. Despite examining several landscape variables, such as wetland proximity to specific crop types, watershed size, and others, land use was the best variable explaining pesticide concentrations in potholes. CRP ameliorated glyphosate in wetlands at concentrations comparable to native prairie and thereby provides another ecosystem service from this expansive program.

Effects of land use on greenhouse gas fluxes and soil properties of wetland catchments in the Prairie Pothole Region of North America.

Wetland restoration has been suggested as policy goal with multiple environmental benefits including enhancement of atmospheric carbon sequestration. However, there are concerns that increased methane (CH4) emissions associated with restoration may outweigh potential benefits. A comprehensive, 4-year study of 119 wetland catchments was conducted in the Prairie Pothole Region of the north-central U.S. to assess the effects of land use on greenhouse gas (GHG) fluxes and soil properties. Results showed that the effects of land use on GHG fluxes and abiotic soil properties differed with respect to catchment zone (upland, wetland), wetland classification, geographic location, and year. Mean CH4 fluxes from the uplands were predictably low (<0.02 g CH4 m(-2) day(-1)), while wetland zone CH4 fluxes were much greater (<0.001-3.9 g CH4 m(-2) day(-1)). Mean cumulative seasonal CH4 fluxes ranged from roughly 0-650 g CH4 m(-2), with an overall mean of approximately 160 g CH4 m(-2). These maximum cumulative CH4 fluxes were nearly 3 times as high as previously reported in North America. The overall magnitude and variability of N2O fluxes from this study (<0.0001-0.0023 g N2O m(-2) day(-1)) were comparable to previously reported values. Results suggest that soil organic carbon is lost when relatively undisturbed catchments are converted for agriculture, and that when non-drained cropland catchments are restored, CH4 fluxes generally are not different than the pre-restoration baseline. Conversely, when drained cropland catchments are restored, CH4 fluxes are noticeably higher. Consequently, it is important to consider the type of wetland restoration (drained, non-drained) when assessing restoration benefits. Results also suggest that elevated N2O fluxes from cropland catchments likely would be reduced through restoration. The overall variability demonstrated by this study was consistent with findings of other wetland investigations and underscores the difficulty in quantifying the GHG balance of wetland systems.

Monitoring and modeling wetland chloride concentrations in relationship to oil and gas development.

Extraction of oil and gas via unconventional methods is becoming an important aspect of energy production worldwide. Studying the effects of this development in countries where these technologies are being widely used may provide other countries, where development may be proposed, with some insight in terms of concerns associated with development. A fairly recent expansion of unconventional oil and gas development in North America provides such an opportunity. Rapid increases in energy development in North America have caught the attention of managers and scientists as a potential stressor for wildlife and their habitats. Of particular concern in the Northern Great Plains of the U.S. is the potential for chloride-rich produced water associated with unconventional oil and gas development to alter the water chemistry of wetlands. We describe a landscape scale modeling approach designed to examine the relationship between potential chloride contamination in wetlands and patterns of oil and gas development. We used a spatial Bayesian hierarchical modeling approach to assess multiple models explaining chloride concentrations in wetlands. These models included effects related to oil and gas wells (e.g. age of wells, number of wells) and surficial geology (e.g. glacial till, outwash). We found that the model containing the number of wells and the surficial geology surrounding a wetland best explained variation in chloride concentrations. Our spatial predictions showed regions of localized high chloride concentrations. Given the spatiotemporal variability of regional wetland water chemistry, we do not regard our results as predictions of contamination, but rather as a way to identify locations that may require more intensive sampling or further investigation. We suggest that an approach like the one outlined here could easily be extended to more of an adaptive monitoring approach to answer questions about chloride contamination risk that are of interest to managers.