RESUMO
Crystallinity of tubular structures of Act. roseoflavus var. roseofungini obtained from both the initial culture and the dedifferentiating fructose mutant was shown with the method of roentgen-phase analysis (the difractometric method with ionization registration of reflection)
Assuntos
Streptomyces/análise , Cristalografia , Difração de Raios XRESUMO
The methods of spectral analysis, spectrophotometry, fluorescence and thin-layer chromatography were used for the study of the composition of the tubular structures of the initial culture of Act. roseoflavus var. roseofungini. It was found that they contained a complex of antibiotics (roseofungin and its analogs), Ca, Mg, Na, K, Si and a significant amount of the third non-identified component in the antibiotic complex.
Assuntos
Microtúbulos/análise , Streptomyces/análise , Dicroísmo Circular , Meios de Cultura , Variação Genética , Metais/análise , Silício/análise , Espectrometria de Fluorescência , Espectrofotometria Infravermelho , Espectrofotometria UltravioletaRESUMO
Tubular formations analogous by their structure to those found on the surface of the outer sheath of the hyphae of the aerial mycelium of Actinomyces roseoflavus var. roseofungini were reconstructed in vitro from the mycelium acetone extract on addition of water. A polyenic antibiotic was shown to be present in the composition of the "tubes". A possible role of the antibiotic in the genesis of the secondary structures of the actinomycete is discussed.
Assuntos
Streptomyces/crescimento & desenvolvimento , Streptomyces/ultraestrutura , Meios de Cultura , Variação Genética , Espectrofotometria Ultravioleta , Streptomyces/análiseRESUMO
Self-organization of various submicroscopic formations, such as "tubes", "ribbons", etc. differing in ultrastructure and containing a polyenic antibiotic was observed in acetone extracts of the mycelium of the "fructose" variant of Actinomyces roseoflavus var. roseofungini. Conditions for separate isolation of the above structures are described. Concentration of the extract and the ratio of the volumes of water added to the extract are determinant for the process of self-organization. A possibility of reorganization of the tubular structures into the ribbon-like ones and vice versa is shown.