An assessment of the occurrence and nutritional factors associated with aflatoxin M1, ochratoxin A, and zearalenone in the breast milk of nursing mothers in Hamadan, Iran.

This research investigated the occurrence of aflatoxin M1 (AFM1), ochratoxin A (OTA), and zearalenone (ZEN) in human milk samples in the Hamadan city, Iran. The study was carried out using the milk of nursing mothers from ten governmental health care centers. Mycotoxin content of ninety milk samples measured using enzyme-linked immunosorbent assay (ELISA). All samples that tested positive for AFM1 with the ELISA test were subsequently analyzed by high-performance liquid chromatography (HPLC). The mean ± SD concentrations of AFM1, determined by ELISA and HPLC were 5.98 ± 1.47 and 4.36 ± 1.23 ng/L, respectively. OTA and ZEN levels were below the detection limit (<5 ng/L) in all samples. None of the contaminated samples exceeded the regulation limit set by the European Commission (25 ng/L) for AFM1 in infant formula. We found a significant correlation between the AFM1 concentration in breast milk and infant age and milk consumption by the nursing mother (p < 0.05). These findings revealed that infants are susceptible to AFM1 exposure from their mother's milk. The authors recommend that additional research be conducted on the analysis of foodstuff and biological fluids for various mycotoxins.

Mercury, Lead, Cadmium, and Barium Levels in Human Breast Milk and Factors Affecting Their Concentrations in Hamadan, Iran.

Breast milk is considered the best source of nutrition for all infants. However, exposure of newborns to toxic metals is of special interest due to their potential harmful effects. Thus, the primary aims of this study were to determine the concentration of toxic heavy metals including lead, mercury, cadmium, and barium in breast milk samples from Hamadan, Iran, in relation to some sociodemographic variables. A total of 100 breast milk samples were collected and their heavy metal contents were measured by inductively coupled plasma mass spectroscopy (ICP-MS). The median breast milk concentrations of Pb, Hg, and Ba were 41.9, 2.8, and 1.95 µg/L, respectively. Cd levels were < 1 µg/L in all samples. The Pb level in 94% of the samples was higher than the recommended Pb limit of < 5 µg/L in breast milk suggested by World Health Organization (WHO). Hg levels in 54% of the breast milk samples were higher than the normal mean concentration (1.7 µg/L) suggested by WHO. We found no correlation between Hg levels in breast milk and sociodemographic factors. Ba levels in all the breast milk samples were lower than the WHO's proposed health-based drinking water guideline (0.7 mg/L). Considering the results of the present study and the vulnerability of infants, along with the well-known toxicity of these metals, further studies are warranted to identify the main sources of exposure that contribute their concentration in breast milk, establish harmless intake values of toxic metals in breast milk, and develop preventive measures.

Exposure to heavy metals released to the environment through breastfeeding: A probabilistic risk estimation.

Toxic heavy metals released into the environment through various anthropogenic activities, raising concerns over their potential adverse effects on human health and the environment. Biological monitoring studies are of great value in toxicological research for estimation of such health effects. The aim of this study was to evaluate the concentrations of lead (Pb), arsenic (As), and chromium (Cr) in the breast milk of urban mothers in Hamadan city, Iran and the accompanying health risk to infants. A total of 100 lactating mothers were recruited from public health care centers in Hamadan city and breast milk samples were collected at 2, 6, 8, and 12 months postpartum. Inductively coupled plasma mass spectroscopy (ICP-MS) has been used to measure target heavy metal concentrations. The median breast milk concentrations of Pb, As, and Cr were 41.90, 0.50, and 3.95 µg/L, respectively. The highest levels of Pb were observed after two months of delivery (with the mean of 96.69 µg/L and median of 45.70 µg/L). Up to 94% of the breast milk samples exceeded the WHO limit suggested for lead contamination of breast milk (<5 µg/L). The breast milk As and Cr contents were above the limit of detection (LOD) in 19% and 76% of samples, respectively. Unacceptable non-cancer health risk levels or hazard quotient (HQ) were found for Pb and As in 61% and 10% of the samples, respectively. Nevertheless, HQ from Cr was <1.0 for 100% of breastfeeding infants. Finally, our results indicated that there may be a potential risk of toxic metals, especially lead, for infants in Hamadan via the consumption of mothers' breast milk.

Analysis of aluminum, minerals and trace elements in the milk samples from lactating mothers in Hamadan, Iran.

The present cross-sectional study is aimed at analyzing the breast milk of lactating mothers in Hamadan, Iran for aluminum and several minerals and trace elements. Ten governmental health care centers were utilized to facilitate collection of breast milk samples. The breast milk samples were collected at 1, 2, 6, 7, and 12 months postpartum from one hundred healthy lactating women, who delivered full-term newborns. Detection of sodium (Na), zinc (Zn), calcium (Ca), iron (Fe), copper (Cu), magnesium (Mg) and aluminum (Al) levels was conducted with the use of Inductively Coupled Plasma Mass Spectrometry (ICP-MS). This method has shown high accuracy, precision, sensitivity, and linearity for the wide range of concentrations. The accumulated data were not normally distributed; thus, the non-parametric Mann-Whitney U test was used in the statistical analysis of the results. Mean concentrations of Fe, Zn, Cu, Ca, Mg, and Na were 0.75, 1.38, 0.35, 255, 34.58, and 155.72 µg/mL, respectively. The mean level of Al, a well-known neurotoxic metal, was determined to be an alarming 0.191 µg/mL. Moreover, 95% of participants contained very harmful concentrations of Al in their milk. This study also revealed Zn deficiency in about 50% of milk samples. Further investigation is needed to elucidate sources of exposure and factors that may influence maternal and fetal exposure to aluminum.