Vascular pathological changes in rat lower extremity and timing of microsurgery after electrical trauma.

The purpose of this study was to form a standard electrical trauma model in rat, to investigate the pathological changes in vessels, and to determine the best day for performing microsurgery. In the preliminary study, 20 rats were divided into five groups. One was control, and the rats in the other four groups were exposed to 240 V electrical potential for 5, 10, 15, and 20 seconds, respectively. Femoral vessels from each group were biopsied for electron and light microscopy. In the study group, 36 rats were subjected to 240 V electrical trauma for 18 seconds. The rats in the study group were divided into three groups. On days 3, 7, and 21, the femoral artery of nine rats in each group were cut and anastomosed. The anastomoses were followed for thrombus formation. Visible, respectively increasing necroses were seen in all animals in the second, third, and fourth preliminary study groups. Light and electron microscopy revealed degeneration of vessel walls and loss of endothelium. Second and third microsurgery study groups had statistically significantly more thrombus. Although after electrical trauma major vessels seem normal, they have pathological changes, and microsurgery success rates are decreased shortly after electrical trauma.

Decrease in cell proliferation by an matrix metalloproteinase inhibitor, doxycycline, in a model of immune-complex nephritis.

AIM: Renal expression of matrix metalloproteinases (MMP) and tissue inhibitors of MMP (TIMP) contribute to the development of tubulointerstitial fibrosis characteristic of progressive forms of primary glomerulonephritis (GN). The aim of this study was to investigate the therapeutic effect of MMP inhibitor, doxycycline, administration in an experimental rat model of immune-complex nephritis (ICN). METHODS: The induction of immune-complex glomerulonephritis was carried out by the administration of an i.v. dose of 2 mg bovine serum albumin (BSA) daily for 28 days after 8 weeks of s.c. immunization with 1 mg of BSA in complete Freund's adjuvant. Doxycycline (30 mg/kg) was given daily (in groups 2 and 4) by gavage for 28 days. RESULTS: Animals treated with doxycycline showed significant reduction in glomerular area and cell proliferation than non-treated controls. Glomerular deposition of immunoglobulin (Ig)G and C3 was less intense in treated rats than non-treated controls. Although not statistically significant, interstitial inflammation was less intense in treated rats than non-treated controls. Glomerular expression of MMP-9 by immunoflourescence was significantly inhibited in the treated group. In addition pro-MMP-2 on gelatin zymography was importantly suppressed by doxycycline in ICN. CONCLUSION: Doxycycline, in addition to its antibiotic property, may, following further investigation, provide a possible survival benefit in proliferative glomerulonephritis.