An examination of anatomic, physiologic, and metabolic factors associated with well-being of broilers differing in field gait score.

An experiment was conducted to evaluate potential differences for indicators of well-being in birds classified as having field gait score (FGS)2 and 3 and to evaluate potential causal factors affecting gait score. In 2 trials, birds with FGS2 and FGS3 were similar for most broiler traits (BW, feed conversion ratio) and fearfulness. Birds with FGS3, however, had improved breast conformation score in both studies and greater breast angle in the second trial compared with birds with FGS2. This improved breast conformation, along with differing ratios of length (hip to neck/hip to tail; P < 0.05), appears to be highly related to gait score. In other words, varying a bird's physical proportions necessitates that the bird's gait changes to maintain center of gravity during locomotion. In trial 2, behaviors were measured to determine if gait score affected behavior. Birds with FGS3 rested more and stood less than those with FGS2. Similar pathological analysis and heterophil:lymphocyte ratio suggest that gait score differences are not due to increased physiological stress or stress-associated pain. The lack of difference in heterophil:lymphocyte ratio, respiratory quotient, and pathology, combined with differing ratios of body proportions and anatomical length ratios, suggests that behavior and gait differences between birds with differing FGS occur with similar levels of well-being.

Predicting effective caloric value of nonnutritive factors: III. Feed form affects broiler performance by modifying behavior patterns.

Two trials of an experiment were conducted to confirm the relationships among effective caloric value (ECV) of the diet, net energy for gain (NEg), BW, feed conversion ratio (FCR), and broiler behavior. Further, we sought to examine such factors with benefits of pelleting, including feed form history (pellets vs. mash) in females from 2 strains of commercial broilers. Composition of gain was measured on a sample of birds in both trials. In trial 1 birds were reared to 23 d on feed in crumble form, when the birds were provided a feed in pellet or mash form for 7 d. Pelleting the feed increased ECV and total NEg, while decreasing eating and increasing resting behavior. Significant correlations (P < 0.05) among resting, NEg, and ECV occurred. In trial 2, birds were reared to 23 d on a crumble diet and then fed diet in mash or pellet form to 36 d. At 37 d of age, half of the birds from each strain and feed form history combination were switched to the alternative feed form. Interactions of strain by grower feed form were present for BW, initial fat, and body energy content indicating that pelleted feed was required for optimum broiler performance of 1 strain. Grower feed form by finisher feed form interactions were present and demonstrated that birds switched from pellets or mash to the alternate feed form consumed more feed in less time than birds that remained on their previous feed form. Significant correlations were observed in both trials between behaviors and FCR and ECV, whereas NEg reflected these differences in trial 1 but not trial 2. Regression analysis indicated that FCR and subsequently ECV were best predicted by lean gain, whereas NEg was best predicted by fat gain. Further, regression analysis established interactive equations in which ECV was predicted (R2 > 0.99) by eating and resting behavior. The results of these trials indicate that the effects of feed form are caused by a modification of behavior patterns, that ECV is responsive to such behavior changes, and that ECV is an effective estimator of the relative caloric value of genetic, management, and husbandry influences.

Components of feed efficiency in broiler breeding stock: influence of water intake and gastrointestinal contents.

An experiment was conducted with 3 lines of broilers to evaluate the potential roles of water intake and fill of the gastrointestinal (GI) tract on feed conversion ratio (FCR) as measured in a 1-wk FCR test. Birds were reared to juvenile selection age (41 d) and subsequently selected, with the best 18 to 29% of male chicks placed into cages for FCR testing. Feed and water intake were highly correlated in all lines, with each in turn highly correlated with weight gain on test. Although filling of the GI tract differed between the top and bottom halves of the FCR distribution, FCR as measured and corrected for weight of GI tract contents were highly correlated (r = 0.59 to 0.93). Differences between FCR classes were striking for weight gain, but differences between FCR class groups were subtle for starting weight and feed intake. These subtle differences are difficult to detect in a single generation, but may add up through generations of selection. Based on the results of the current and previous studies, it appears that selection for improved FCR works at multiple levels to improve efficiency of feed conversion in broiler breeding stock.

Predicting effective caloric value of nonnutritive factors: I. Pellet quality and II. Prediction of consequential formulation dead zones.

Two experiments were conducted with male broilers to 1) establish a methodology for predicting effective caloric value (ECV), defined as dietary caloric density (CD) necessary for broilers to achieve specific BW and feed conversion ratio (FCR) combinations under standardized conditions and 2) quantify the ECV attributable to pellet quality (PQ), defined as the pellet to pellet fines ratio in the feeder. In experiment 1, chicks were reared to 56 d on diets varying in CD. Dietary caloric densities examined ranged from 2,650 to 3,250 kcal of MEn/kg. Pen BW, feed intake, and FCR were measured at 21, 42, and 56 d. On 42 and 56 d, carcass traits were measured. Increasing CD significantly enhanced BW, energy consumption, and FCR. Feed intake remained similar across the upper 3 CD treatments to 42 d. By d 56, feed consumption tended to decline as CD increased. Increasing CD beyond 3,066 kcal of MEn/kg diet did not increase lean tissue accretion, while fat deposition rose disproportionately. Experiment 1 results enabled development of equations whereby CD, hence ECV, might be predicted using BW and FCR. In experiment 2, 38-d-old broilers were used to evaluate PQ effects on growth, feed intake, FCR, and behavior in a 7-d FCR assay. The BW gain and FCR were significantly enhanced by pelleting and were positively correlated with PQ. Feed intake was not affected by PQ. The experiment 1 model was validated for experiment 2, as it closely estimated the CD for diets of similar PQ used in experiment 1. Results suggest pelleting contributes 187 kcal/kg of diet at 100% PQ and that the ECV declines curvilinearly as PQ falls. Birds were observed eating less and resting more as PQ increased, suggesting that ECV of pelleting is mediated by energy expenditure for activity. These studies provide a method for estimating ECV of nonnutritive factors that impact BW, FCR, or both. Further, the application reveals potential for creation of formulation "dead zones" whereby dietary changes to enhance CD may be offset due to reduced ECV.

Components of feed efficiency in broiler breeding stock: the use of fasted body temperature as an indicator trait for feed conversion in broiler chickens.

Five trials were conducted to evaluate the usefulness of body temperature (BT) measured under conditions of mild handling stress as an indicator trait for feed conversion ratio (FCR) in broilers. The trials used males from 4 lines of broilers (a randombred control population and 3 heavy broiler lines). Birds were reared to approximately 6 wk of age and then selected for FCR testing based upon BW and conformation. At the start of the FCR test, birds were fasted for periods lasting from 18 to 48 h, and their BT were measured, which was followed immediately by initiation of an FCR test lasting 1 wk. The correlation between FCR and BT was significant in only 2 of the 5 trials. Differences between good and poor converters were generally attributed to greater gains of BW on similar amounts of feed intake. The results of the current trials indicate gain on test is the primary factor affecting FCR and that BT is not a reliable predictor of FCR.

An evaluation of endo-beta-D-mannanase (Hemicell) effects on broiler performance and energy use in diets varying in beta-mannan content.

Two experiments were conducted to evaluate the effects of a commercial endo-beta-D-mannanase (Hemicell) on overall performance, MEn, net energy for gain, and some serum parameters of broilers fed diets varying in beta-mannan level (experiment 1) and to evaluate effects of enzyme level on the same variables in broilers fed diet high in beta-mannan (experiment 2). As a semipurified beta-mannan source, guar gum was used to alter the dietary beta-mannan level. In experiment 1, guar gum was added at 0, 0.5, 1, and 2% in a corn-soy-based starter diet with (0.05%) and without endo-beta-D-mannanase supplementation in a 4 x 2 factorial design. Enzyme supplementation improved (P < 0.01) feed efficiency at control and each guar gum inclusion level, whereas 2% guar gum supplementation reduced (P < 0.01) BW and increased (P < 0.01) 14-d feed:gain ratio. Enzyme supplementation also increased dietary MEn and net energy gain. In experiment 2, endo-beta-D-mannanase was added at 0, 0.5, 1, and 1.5% in a corn-soy-based starter diet containing 1% guar gum. Increasing endo-beta-D-mannanase supplementation did not affect (P > 0.10) final BW but improved 14-d feed:gain ratio at all inclusion levels. As in the first experiment, ME improved (P < 0.05) with increasing enzyme inclusion. Dietary endo-beta-D-mannanase inclusion significantly reduced water:feed ratio and total dry fecal output (P < 0.01). Taken together, the results of these 2 experiments indicate that endo-beta-D-mannanase supplementation may improve the utilization of nutrients in diets containing beta-mannan.

Graded atmospheric oxygen level effects on performance and ascites incidence in broilers.

The effects of graded atmospheric O2 concentration (12, 14, 16, 18, and 20.6%) on chick performance and propensity to develop ascites were investigated using commercial male broilers. Chicks were housed in calorimetry chambers for 2 wk with incoming air diluted with N to provide the desired O2 concentration at thermoneutral (TN) ambient temperature. Day 14 body weight, weight gain, feed consumption, and gain-to-feed ratio increased (P < 0.01) as O2 concentration incrementally rose from 12 to 20.6%. Body weight was 138 g for the lowest atmospheric O2 level compared to 371 g for 20.6% O2. The greatest treatment difference occurred between the 12 and 14% O2 concentrations. Growth depression appeared related to feed consumption. Ascites heart ratio (AHR), ascites score (AS), right ventricular mass (RVM), and hematocrit (HCT) all increased (P < 0.01) as O2 concentration decreased. Blood HCT appeared to be a more sensitive indicator of physiological change attributable to atmospheric O2 than AHR, AS, or RVM. The data reported herein suggests that 19.6% atmospheric O2 is the minimal allowable level for housing birds within a relatively stress-free, TN environment to avoid cardiac and HCT changes related to ascites.

Components of feed efficiency in broiler breeding stock: energetics, performance, carcass composition, metabolism, and body temperature.

Two experiments were conducted to identify factors influencing feed conversion in broilers. The first experiment was conducted to develop a test for body temperature (BT) under mild handling stress. In the first experiment, BT was recorded periodically with birds briefly restrained after they had been removed from feed with either continuous lighting or 16L:8D. Body temperature was affected by photoperiod and declined following feed removal. The second experiment was conducted to investigate metabolic-physiologic differences existing between good (G) and poor (P) converting broilers, initially classified in a commercial breeding company's feed conversion (FCR) test. Basal metabolic rate (BMR) and BT were measured at the beginning and end of the FCR test following appropriate time without feed. Heat production (HP) was recorded throughout the study both for BMR calculations and to measure net energy (NE). Excreta were collected for measurement of AME, and at study termination, birds were analyzed for carcass composition. The G and P converters, however, had similar BMR and feed consumption but differed in AME (58 kcal/kg) and total NE (184 kcal/kg). Generally, G and P broilers had similar starting BW, but the G broilers gained more weight on the same amount of feed than did P broilers. The two BT measurements recorded in the present study were correlated with each other. The BT was also correlated with subsequent gain and FCR, indicating potential use as an indicator trait for feed conversion. Further, the data indicated that processes other than basal metabolism (measured as average hourly HP) may impact FCR. These might include variability in fear response during BT measurement or variations in bird activity level.

Components of feed efficiency in broiler breeding stock: is improved feed conversion associated with increased docility and lethargy in broilers?.

Two trials were conducted to test the hypothesis that broilers classified as good rather than poor feed converters (low vs. high feed-to-gain ratios, respectively) would show decreased activity (increased lethargy) and reduced fear of human caretakers. In both trials birds were reared to juvenile selection age when the 192 males with the "best" breast and leg conformation were placed into individual bird cages (46 x 60 cm), and their feed conversion ratio (FCR) was measured. In trial 1, birds remained in the cages for 7 d for assessment of feed conversion. During the feed conversion testing period, the birds were subjected to three behavioral tests. First, approach-avoidance responses to a novel object placed in the feeder were observed when feeding would be expected. Second, behavior patterns of individual birds were assessed by recording feeding, drinking, walking pecking, preening, standing, and resting. Third, the 20 birds showing the best FCR and the 20 showing the worst were induced into tonic immobility (TI). Feed conversion was not related to either the novel object or the TI measures of fearfulness. Neither was FCR related to feeding, drinking, walking, or pecking. However, FCR was positively correlated with both standing and preening and negatively correlated with resting behavior. In trial 2, the FCR test lasted for 11 d. The birds were assessed for behavioral patterns (feeding, drinking, walking, pecking, preening, standing, and resting) as in the previous trial. As in trial 1, FCR was positively correlated with standing and negatively correlated with resting behavior. Contrary to expectations, the results of these two studies suggest that broilers with good FCR were actually less lethargic and no more fearful than those that showed poor FCR values.

The incidence of tuberculosis in drug users with small tuberculin reaction sizes.

SETTING: In persons infected with the human immunodeficiency virus (HIV), a decreased tuberculin reaction cut-point of > or = 5 mm induration is recommended. OBJECTIVE: To determine tuberculosis risk in non-anergic HIV-infected persons with 5-9 mm tuberculin reactions. DESIGN: A prospective study with semi-annual tuberculin and anergy testing, HIV antibody and T cell subset assays, and active surveillance for tuberculosis. RESULTS: Participants were 572 HIV-seronegative and 241 HIV-seropositive non-anergic drug users. No tuberculosis occurred in HIV-seronegative persons. Tuberculosis incidence among HIV-seropositive drug users was 3.3, 7.7, 0, and 0.34 per 100 person-years in those with tuberculin reaction sizes of > or = 10 mm, 5-9 mm, 1-4 mm, and 0 mm, respectively, and was significantly increased in persons with 5-9 mm induration compared with those with 0-4 mm induration (rate ratio 27.7, 95%CI 2.9-268). Among persons with reaction sizes of 5-9 mm, tuberculosis occurred exclusively in those with CD4+ lymphocyte counts <500/mm3 at the time of their 5-9 mm tuberculin reactions. CONCLUSION: HIV-infected persons with tuberculin reaction sizes of 5-9 mm are at increased risk for tuberculosis compared to non-anergic persons with smaller (0-4 mm) reaction sizes. However, this increased risk may be limited to those with low CD4+ lymphocyte counts at the time of tuberculin testing.

Self-assessment of tuberculin skin test reactions by drug users with or at risk for human immunodeficiency virus infection.

SETTING: Self-assessment of tuberculin test results, if accurate, could enhance tuberculosis screening efforts by reducing the need for follow-up visits for skin test reading. We investigated tuberculin test self-assessment in a longitudinal study of tuberculosis infection among drug users. OBJECTIVE: To determine the accuracy of tuberculin reaction self-assessment by drug users at high risk for tuberculosis infection. DESIGN: Two readings were compared of the same skin test, performed 48-72 hours after placement: 1) self-assessment using a simple yes-no approach to induration, versus 2) trained examiner reading. Self-assessments were performed immediately prior to trained examiner readings. RESULTS: Participants were 137 human immunodeficiency virus (HIV) seropositive and 344 HIV-seronegative current and former drug users. Ten per cent (35/344) of reactions read by participants as 'flat' were read by trained examiners as > or =5 mm (54% of which were > or =10 mm). Twenty-three per cent (19/82) of reactions read by trained examiners as > or =10 mm and 32% (35/110) of reactions read by trained examiners as being > or =5 mm were self-read by participants as 'flat'. Sensitivity (0.68) and specificity (0.83) of self-read tuberculin reactions were sub-optimal. Inter-reader reliability was poorer between participants and trained examiners than between trained examiners. CONCLUSION: Self-assessments of tuberculin skin test responses by drug users with or at risk for HIV infection are not reliable.

Virginiamycin and caloric density effects on live performance, blood serum metabolite concentration, and carcass composition of broilers reared in thermoneutral and cycling ambient temperatures.

One experiment utilizing Cobb x Cobb male broilers was conducted to evaluate virginiamycin (VM; 0, 15, 20 ppm) and diet caloric density (CD; 2,945, 3,200 AMEn/kg) effects on broiler live performance, blood serum metabolites, and carcass composition. The starter period exposed birds to recommended brooding conditions, whereas from 3 to 7 wk birds were exposed to thermoneutral (TN, 24 C) or cycling temperature (CT, 24 to 35 C) environments (E). During the 21-d starter period, VM levels and high CD increased (P < 0.05) BW gain (G) and gain:feed (G:F) improved (P < 0.05) with 20 ppm VM and high CD. During 3 to 7 wk, CT reduced (P < 0.05) most live performance and carcass variables as well as heat production (HP) and energetic efficiency whereas energy content per gram of tissue increased. The main effect of VM and CD on blood serum constituents was not significant; however, CT decreased (P < 0.05) serum Na, K, Ca, Mg, Fe, albumin, and total protein. Within CT, G increased (P < 0.05) with high CD and with the 15 ppm VM combination, whereas within TN, G was unaffected by CD, but increased (P < 0.05) with the 20 ppm VM and low CD combination compared with the control. High CD increased (P < 0.05) BW, G, carcass weight, dressing percentage, carcass percentage fat, carcass dry matter, carcass energy content per bird, HP, fat, and protein gain but reduced (P < 0.05) carcass percentage protein and energetic efficiency. Carcass weight, breast yield, fat, and protein gains as well as dry matter carcass energy content increased (P < 0.05) with VM compared with controls. The reduced (P < 0.05) calorie intake and HP with concomitant increase (P < 0.05) in calorie gain with 20 ppm VM increased (P < 0.05) caloric efficiency. In summary, the results suggest that VM improves bird performance by reducing HP and that reduced HP during high CT improves body temperature homeostasis.

Examination of the dietary methionine requirements of breeding northern bobwhite, Colinus virginianus.

Adult Northern bobwhite were used to test the hypothesis that dietary methionine levels recommended by the NRC for breeding quail are excessive for wild bobwhite. We tested the hypothesis by comparing immunocompetence, reproductive performance, and chick viability of Northern bobwhite hens fed diets containing low (0.31%), moderate (0.39%), or high (0.47%) concentrations of methionine. Chick viability was determined by assessing immunocompetence, including evaluating the ability of hens to passively transfer immunity to their chicks. Hens were fed the experimental diets for 6 wk on an ad libitum basis. After 6 wk, methionine treatment had no measurable effect (P > or = 0.20) on hen phytohemagglutinin wing web indices, organ weights, or serum anti-Pasteurella multocida titer indices. Mean egg weight, percentage egg production, total cumulative egg production, yolk weight, yolk volume, and percentage fertile and percentage hatch of fertile eggs did not differ (P > or = 0.12) among diet treatments. Amount of albumen in eggs produced by hens fed the high methionine diet averaged 0.27 g more (P = 0.003) than eggs of hens fed the low methionine diet. Anti-P. multocida titer of yolks from eggs in Week 6 were not different (P = 0.36) between birds fed the high and the low methionine diets. The mortality rate of chicks after challenge with 23 cfu of P. multocida was not different (P > or = 0.05) among diets. Chicks hatched from eggs laid by vaccinated hens during Weeks 2 and 3, however, had lower (P < 0.05) mortality than chicks of unvaccinated hens. It appears a dietary methionine concentration of 0.3% may be sufficient for wild Northern bobwhite to produce viable chicks.

Acclimation effects on fed and fasted broiler thermobalance during thermoneutral and high ambient temperature exposure.

1. Two experiments were conducted to quantify heat stress (HS) acclimation effects on heat production (H), evaporative heat loss (E), sensible heat loss (S) and change in heat content (HC) of 24 food-deprived and precision fed broilers. 2. In experiment 1, heat stressed fasted HS acclimated birds (group 1) exhibited lower H (22.6 v. 25.5 kJ/kgW0.66 per h), E (7.5 v. 8.8 kJ/kgW0.66 per h), core body temperature (41.8 v. 42.4 C) and respiration rate (129 v. 160 breaths/min) than nonacclimated controls (group 2). 3. In contrast to the first experiment, precision fed HS acclimated birds (group 1) exhibited a higher H (29.3 v. 28.0 kJ/kgW0.66 per h) and E (10.5 v. 9.6 kJ/kgW0.66 per h) during HS and elevated H during thermoneutral periods than their non-acclimated counterparts (group 2). The elevated H became more pronounced with each successive HS exposure. 4. These results indicate that H increases when broilers are fed, that broilers preferentially dissipate heat as S when environmental conditions permit, and that food and/or energy consumption level markedly influences the bird's capacity to exhibit a HS acclimation response, and in fact has the ability to mask it.

Effects of ambient temperature on broiler mineral balance partitioned into urinary and faecal loss.

1. Two experiments were conducted on control (intact) and colostomised 4 to 7 week old broilers to evaluate the influence of 24 degrees C, diurnally cycling 24 to 35 degrees C and chronic 35 degrees C ambient temperatures on broiler mineral balance, urinary and faecal mineral excretion and urinary osmolar characteristics. 2. In the first experiment, colostomy had no significant effect on mineral balance. However, broilers exposed to high cycling ambient temperature reduced their retention of phosphorus, potassium, sodium, magnesium, sulphur, manganese, copper and zinc compared with birds housed at 24 degrees C. 3. Despite the minimal effect of high ambient temperature on urine production, minerals excreted disproportionately excreted in urine included potassium, magnesium, phosphorus and sulphur while copper and magnesium were lost primarily via the faeces. 4. In the second experiment, exposure to 35 degrees C increased urine output from 50.7 ml/12 h per kg of body weight at 24 degrees C to 101.3 ml/12 h per kg of body weight and was associated with an increased urine:water ratio and reduced urine osmolality. 5. Reduced urinary chloride and higher potassium, phosphorus, sulphur, sodium, magnesium, calcium and manganese excretion was observed for broilers housed in under high ambient temperatures compared to 24 degrees C. 6. These studies suggest that high ambient temperatures adversely influence mineral metabolism and, furthermore, that the route of excretion varies with the specific mineral and the environmental temperature exposure.

Vitamin and trace mineral withdrawal effects on broiler breast tissue riboflavin and thiamin content.

An investigation was carried out to determine whether a Day 28 to 49 dietary vitamin, trace mineral, and vitamin plus trace mineral premix withdrawal would impact Pectoralis major thiamin or riboflavin concentration in chicks reared under thermoneutral (24 C) and heat-stressed (24 to 35 C) conditions. No significant (P > 0.1) environment by nutrient withdrawal interactions were detected. Heat stress and vitamin withdrawal reduced (P < 0.05) P major thiamin and riboflavin concentration. In contrast, trace mineral withdrawal failed (P> 0.1) to impact either vitamin. In conclusion, results from this study suggest that a 21-d vitamin withdrawal and heat stress exposure have the potential to reduce muscle riboflavin and thiamin concentration.

Bronchodilator, oxygen level, and temperature effects on ascites incidence in broiler chickens.

Oxygen level, ambient temperature, and bronchodilator (metaproterenol) effects on performance, ascites incidence, and hematology were investigated using commercial broilers. In Experiment 1, two atmospheric oxygen concentrations (17.6, 20.6%) and two ambient temperatures (26.7, 32.2 C) were examined in a factorial arrangement. The low-oxygen (17.6%), ambient temperature combination (26.7 C) reduced (P < .01) weight gain whereas the high-oxygen ambient temperature combination enhanced (P < .05) such performance. Ascites incidence for chicks housed at 32.2 C was higher (P < .01) for birds maintained at 17.6% oxygen but oxygen concentration did not impact ascites incidence at 26.7 C. White blood cell counts were elevated in the low-oxygen (17.6%) group at both 26.7 and 32.2 C, and hematocrit was positively (R = .84; P < .05) correlated with ascites incidence. In Experiment 2, two oxygen levels (17.6, 20.6%) at constant 26.7 C were examined with and without metaproterenol, a bronchodilator. Metaproterenol reduced (P < .01) ascites incidence at 17.6% oxygen and completely prevented ascites at 20.6% oxygen. The data suggest that therapeutic development may be enhanced by using hematocrit as an indices of ascites and further that studies directed at bronchodilation have potential to alleviate ascites.

Effect of heat stress and drinking water salt supplements on plasma electrolytes and aldosterone concentration in broiler chickens.

An experiment was conducted to evaluate the effects of supplementing drinking water with isomolar (0.067 mol/l) KCl or NaCl on mass gain, food and water consumption, rectal temperature, and plasma concentrations of aldosterone, Na+, and K+ in broiler chickens reared in thermoneutral and cycling heat stressing environments. Heat stress decreased (P > or = 0.05) mass gain, food consumption, and plasma concentrations of Na+ and K+, while increases (P < or = 0.05) in plasma concentrations of aldosterone, rectal temperature, and water consumption were observed. Drinking water supplemented with either KCl or NaCl increased (P < or = 0.05) broiler mass gain and water consumption, but had no effect (P > 0.1) on the other variables evaluated. The results of this study indicate that broiler chickens in a heat stress environment are under osmotic stress and supplementing drinking water with 0.067 mol/l KCl or NaCl does not lessen this stress.

Effect of oxygen level on ascites incidence and performance in broiler chicks.

An experiment was conducted using male Cobb x Cobb broiler chicks to evaluate the effect of two oxygen levels on the incidence of ascites, performance, and blood chemistry. Ascites occurred in the 13.6% oxygen groups on the fifth day of the experiment. Body weights and survivability were significantly lower (P < 0.05) in chicks maintained at the low oxygen level than in controls (20.6% oxygen). Hematocrits were significantly higher only in chicks with ascites at the 13.6% oxygen level. Hemoglobin was significantly higher (P < 0.05) in chicks with ascites at 13.6% oxygen than in chickens at 20.6% oxygen. Lactate dehydrogenase values were significantly higher in non-ascitic chicks maintained at 13.6% oxygen and in chicks with ascites at 20.6% oxygen than in non-ascitic chicks 20.6% oxygen.

Nicarbazin effects on broiler thermobalance during high ambient temperature stress.

Two experiments were conducted to measure the effects of nicarbazin (125 ppm) on heat production (H), evaporative heat loss (E), sensible heat loss (S), and heat content change (HC) of broilers during heat stress. Feed consumption effects on thermobalance were equalized in both studies by force feeding at 7% of metabolic body weight (MWT; body weight.66) daily. In Experiment 1, using broilers not acclimated to heat stress, nicarbazin increased H (P < .05) (9.5 vs. 9.0 kcal/h per MWT) and body temperature (P = .08), reduced (P < .05) respiration rate, and had no impact on E or S (P > .1). In contrast, the bird acclimated to heat stress used in Experiment 2 exhibited similar (P > .1) thermobalance responses irrespective of nicarbazin supplementation. The data suggests that heat-stress-mediated nicarbazin toxicity may be related to H and further that nicarbazin's heat-stress-mediated toxicity is reduced in HS-acclimated chicks.