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1.
J Strength Cond Res ; 30(2): 311-9, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23604000

RESUMO

Biomarkers of inflammation, muscle damage, and oxidative stress after high-intensity exercise have been described previously; however, further understanding of their role in the postexercise recovery period is necessary. Because these markers have been implicated in cell signaling, they may be specifically related to the training adaptations induced by high-intensity exercise. Thus, a clear model showing their responses to exercise may be useful in characterizing the relative recovery status of an athlete. The purpose of this study was twofold: (a) to investigate the time course of markers of muscle damage and inflammation in the blood from 3 to 72 hours after combined training exercises and (b) to investigate indicators of oxidative stress and damage associated with increased reactive oxygen species production during high-intensity exercise in elite athletes. Nineteen male athletes performed a combination of high-intensity aerobic and anaerobic training exercises. Samples were acquired immediately before and at 3, 6, 12, 24, 48, and 72 hours after exercise. The appearance and clearance of creatine kinase and lactate dehydrogenase in the blood occurred faster than previous studies have reported. The neutrophil/lymphocyte ratio summarizes the mobilization of 2 leukocyte subpopulations in a single marker and may be used to predict the end of the postexercise recovery period. Further analysis of the immune response using serum cytokines indicated that high-intensity exercise performed by highly trained athletes only generated inflammation that was localized to the skeletal muscle. Biomarkers are not a replacement for performance tests, but when used in conjunction, they may offer a better indication of metabolic recovery status. Therefore, the use of biomarkers can improve a coach's ability to assess the recovery period after an exercise session and to establish the intensity of subsequent training sessions.


Assuntos
Exercício Físico/fisiologia , Esforço Físico/fisiologia , Adulto , Biomarcadores/metabolismo , Catalase/sangue , Creatina Quinase/sangue , Citocinas/sangue , Humanos , L-Lactato Desidrogenase/sangue , Contagem de Leucócitos , Masculino , Neutrófilos/metabolismo , Recuperação de Função Fisiológica/fisiologia
2.
Comp Biochem Physiol C Toxicol Pharmacol ; 154(3): 226-33, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21699995

RESUMO

In the present study, an acidic PLA(2), designated Bl-PLA(2), was isolated from Bothrops leucurus snake venom through two chromatographic steps: ion-exchange on CM-Sepharose and hydrophobic chromatography on Phenyl-Sepharose. Bl-PLA(2) was homogeneous on SDS-PAGE and when submitted to 2D electrophoresis the molecular mass was 15,000Da and pI was 5.4. Its N-terminal sequence revealed a high homology with other Asp49 acidic PLA(2)s from snake venoms. Its specific activity was 159.9U/mg and the indirect hemolytic activity was also higher than that of the crude venom. Bl-PLA(2) induced low myotoxic and edema activities as compared to those of the crude venom. Moreover, the enzyme was able to induce increments in IL-12p40, TNF-α, IL-1ß and IL-6 levels and no variation of IL-8 and IL-10 in human PBMC stimulated in vitro, suggesting that Bl-PLA(2) induces proinflammatory cytokine production by human mononuclear cells. Bothrops leucurus venom is still not extensively explored and knowledge of its components will contribute for a better understanding of its action mechanism.


Assuntos
Bothrops , Venenos de Crotalídeos/enzimologia , Inflamação/metabolismo , Fosfolipases A2/química , Fosfolipases A2/farmacologia , Sequência de Aminoácidos , Animais , Células Cultivadas , Cromatografia por Troca Iônica/métodos , Citocinas/metabolismo , Edema/induzido quimicamente , Eletroforese em Gel de Poliacrilamida , Humanos , Interleucina-10/metabolismo , Subunidade p40 da Interleucina-12/metabolismo , Interleucina-6/metabolismo , Interleucina-8/metabolismo , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/metabolismo , Masculino , Camundongos , Peso Molecular , Fosfolipases A2/isolamento & purificação , Sefarose/análogos & derivados , Homologia de Sequência de Aminoácidos , Fator de Necrose Tumoral alfa/metabolismo
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