Inflammatory biomarkers responses after acute whole body vibration in fibromyalgia.

The aims of this study were 1) to characterize the intensity of the vibration stimulation in women diagnosed with fibromyalgia (FM) compared to a control group of healthy women (HW) matched by age and anthropometric parameters, and 2) to investigate the effect of a single session of whole body vibration (WBV) on inflammatory responses. Levels of adipokines, soluble tumor necrosis factor receptors (sTNFr1, sTNFr2), and brain-derived neurotrophic factor (BDNF) were determined by enzyme-linked immunosorbent assay. Oxygen consumption (VO2) was estimated by a portable gas analysis system, heart rate (HR) was measured using a HR monitor, and perceived exertion (RPE) was evaluated using the Borg scale of perceived exertion. Acutely mild WBV increased VO2 and HR similarly in both groups. There was an interaction (disease vs vibration) in RPE (P=0.0078), showing a higher RPE in FM compared to HW at rest, which further increased in FM after acute WBV, whereas it remained unchanged in HW. In addition, there was an interaction (disease vs vibration) in plasma levels of adiponectin (P=0.0001), sTNFR1 (P=0.000001), sTNFR2 (P=0.0052), leptin (P=0.0007), resistin (P=0.0166), and BDNF (P=0.0179). In conclusion, a single acute session of mild and short WBV can improve the inflammatory status in patients with FM, reaching values close to those of matched HW at their basal status. The neuroendocrine mechanism seems to be an exercise-induced modulation towards greater adaptation to stress response in these patients.

Evaluation of textile dye degradation due to the combined action of enzyme horseradish peroxidase and hydrogen peroxide.

The kinetic parameters of the oxidant action of the combination of enzyme horseradish peroxidase (HRP) with hydrogen peroxide in the degradation of methylene blue dye were investigated. Twenty-one percent of color removal was obtained at pH 5.0 and temperature of 30 °C. Under these conditions, the kinetic parameters K m and V max of enzymatic reactions were determined for hydrogen peroxide in the absence of methylene blue dye (K m = 17.3 mM; V max = 1.97 mM/min) and in the presence of methylene blue dye (K m = 0.27 mM, V max = 0.29 µM/min). By means of analysis of phosphorescence, the presence of reactive oxygen species was detected in the form of singlet oxygen through the redox reaction between HRP and hydrogen peroxide. The existence of this reactive species is directly dependent on the concentration of hydrogen peroxide in the aqueous solution.

Influence of experimental variables on decoloration of azo reactive dyes by hydrogen peroxide and UV radiation.

This paper was intended to study decoloration of azo C.I. Reactive Red 243 under UV radiation in the presence of hydrogen peroxide. The H2O2/UV oxidation process is a feasible and effective alternative in the treatment of effluents that contain such recalcitrant substances as reactive dyes. A statistical factorial design at 2n was used to evaluate how the variables pH (3 and 11), H2O2 concentration (5 and 20 mM), urea concentration (5 and 15 g l(-1)), and NaCl concentration (2.5 and 3.5 g l(-1)) affected the decoloration process efficiency. The results were expressed as percent colour removal from the solution by using absorbance measurements. The H2O2/UV process proved to be effective in degrading reactive dyes, with approximately 99% colour removal being achieved under specific conditions. Hydrogen peroxide concentration had a positive effect and influenced a great deal. Sodium chloride (NaCl) concentration presented a negative effect on the experiments in which it reduced the percent colour removal from the reactive dye solutions. Solution pH and urea concentration did not present any significant effect on the decoloration process.