[Lipoxygenase oxidation of arachidonic acid in murine splenocytes and its modulation by the lactone ganglioside GM3]. / Lipoksigenaznoe okislenie arakhidonovoi kisloty v splenotsitakh myshei i ego modulirovanie laktonom gangliozida GM3.

The main arachidonic acid metabolites released into the medium by mouse splenocytes have been identified on the basis of chromatographic and spectral studies as well as by mass spectrometry of the derivatives. In the absence or presence of exogenous arachidonic acid mouse splenocytes produce mainly 12-hydroxy-5,8,10,14-eicosatetraenoic and 12,20-dihydroxy-5,8,10,14-eicosatetraenoic acids. Both products are constantly released by intact cells into surrounding media without stimulation by exogenous substrate or other modulators. For the first time it is shown that exogenously added ganglioside GM3 lactone as well as ganglioside GM3 itself can influence the arachidonic acid metabolism in splenocytes.

[Ganglioside lactones in human stomach and breast tumors]. / Laktony gangliozidov v opukholiakh zheludka i molochnoi zhelezy cheloveka.

Ganglioside lactones absent in homologous normal tissues have been found in minute amounts in human gastric and mammary tumours. In mammary gland tumours only the ganglioside GM3 lactone has been identified. Gastric tumours also contain the GM3 lactone; in one case a ganglioside GD3 lactone was identified.

[Ganglioside (GD3) in serum of cancer patients]. / Gangliozid (GD3) v syvorotke krovi rakovykh bol'nykh.

Gangliosides were studied in blood serum of healthy volunteers and of patients with cancer of mammary gland and stomach. Blood serum of the majority of patients with cancer and only 15% of healthy persons were shown to contain ganglioside GD3 which was detected in blood of patients with some other tumors.

[Gangliosides GM3 and GD3 in human stomach and breast tumors]. / Gangliozidy GM3 i GD3 v opukholiakh zheludka i molochnoi zhelezy cheloveka.

Gangliosides of human gastric and mammary tumours and of homologous normal tissues were studied by using biochemical methods and specific antisera. It was found that in most cases GM3, GD3 and GM1 are predominant gangliosides, whereas several polar components are minor ones. A comparison of the relative amount of ganglioside fractions revealed that in gastric tumours the per cent content of polar compounds is higher than in intact tissue; however, the absolute content of all gangliosides is markedly increased. A comparative study of the composition of mammary tumour and normal tissue gangliosides demonstrated two types of changes: i) the absolute content of all gangliosides in tumour tissue was increased and, ii) the increase in the content of total gangliosides was paralleled with the appearance of a new fraction (presumably GM4), the decrease of the GD3 content and the disappearance of polar gangliosides. A possible mechanism of this effect is discussed.