RESUMO
The content of ischemia-modified albumin (IMA), serum albumin, and antioxidant capacity of blood serum was studied in healthy newborns and in newborns with moderate and severe asphyxia on days 1-2 and 3-4 of the postnatal period. Changes in these indicators were found in both groups of newborns with birth asphyxia in comparison with the group of healthy newborns and were more pronounced in children with severe asphyxia. An increase in the IMA level (by 1.6 times; p<0.001) and antioxidant capacity of blood serum (by 2.4 times; p<0.001) and a decrease in serum albumin content (by 1.5 times; p<0.001) were found in severe asphyxia on days 1-2. Analysis of changes in these indicators by days 3-4 allows to talk about a decrease in the intensity of free-radical reactions in newborns with birth asphyxia during complex therapy.
Assuntos
Antioxidantes , Albumina Sérica , Criança , Humanos , Recém-Nascido , Biomarcadores , Antioxidantes/uso terapêutico , Asfixia , Estudos de Casos e Controles , Estresse OxidativoRESUMO
We studied the ability of an aqueous extract from yerba mate and a dry extract obtained on the basis of this aqueous extract to remove Fe(II) ions from an aqueous medium. Aqueous extracts from mate dose-dependently reduced the concentration of free Fe(II) ions assayed by the reaction with 1,10-phenanthroline. This can be attributed to polyphenolic compounds with iron-chelating properties present in aqueous extracts from mate, namely quercetin, rutin, caffeic and chlorogenic acids. These substances effectively removed Fe(II) ions from the medium (the initial concentration of these ions was 15 µM) in the concentration range of 20-30 µM. Binding of Fe(II) ions by aqueous mate extracts (due to the formation of chelate complexes with the participation of polyphenolic compounds) modified their absorption spectra in the visible region. Binding of Fe(II) ions can be a mechanism of the antioxidant action of yerba mate.
Assuntos
Ilex paraguariensis , Ilex paraguariensis/química , Extratos Vegetais/química , Ferro , Íons , Compostos FerrososRESUMO
TLR2-mediated ROS production by mouse peritoneal macrophages was studied by luminoldependent chemiluminescence under conditions of cell stimulation with zymosan (TLR2/6 ligand) and peptidoglycan (TLR2/1 ligand). ROS production by macrophages stimulated with zymosan and peptidoglycan simultaneously depended on the ratio of ligand concentrations. Three effects were revealed: additivity of the stimulating effects of the ligands used, competitive ligand binding, and effect of macrophage priming with peptidoglycan during cell stimulation with zymosan. The mechanisms of these effects are discussed.
Assuntos
Macrófagos Peritoneais/efeitos dos fármacos , Macrófagos Peritoneais/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Receptor 2 Toll-Like/agonistas , Receptor 2 Toll-Like/metabolismo , Animais , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Peptidoglicano/farmacologia , Zimosan/farmacologiaRESUMO
Dihydroquercetin is a natural flavonoid with antioxidant properties that are determined by its ability to scavenge free radicals and chelate transition metal ions. Chemiluminescent studies showed that iron (II) ions in complex with dihydroquercetin are inactive and cannot catalyze decomposition of hydrogen peroxide (Fenton reaction).
Assuntos
Sequestradores de Radicais Livres/química , Peróxido de Hidrogênio/química , Radical Hidroxila/antagonistas & inibidores , Ferro/química , Quercetina/análogos & derivados , Catálise , Concentração de Íons de Hidrogênio , Radical Hidroxila/química , Cinética , Oxirredução , Quercetina/química , SoluçõesRESUMO
We revealed a relationship between water balance and LPO in the myocardium, liver, and blood plasma during massive blood loss and irradiation with He-Ne laser. Low-intensity laser irradiation of the plasma inhibits LPO and normalizes water balance in rat tissues during massive blood loss.
