Assuntos
Odontólogos/legislação & jurisprudência , Pessoas com Deficiência/legislação & jurisprudência , Infecções por HIV/transmissão , Transmissão de Doença Infecciosa do Paciente para o Profissional/legislação & jurisprudência , Decisões da Suprema Corte , Humanos , Transmissão de Doença Infecciosa do Paciente para o Profissional/estatística & dados numéricos , Recusa em Tratar/legislação & jurisprudência , Risco , Estados UnidosAssuntos
Infecções por HIV/transmissão , Adulto , Criança , Feminino , HIV-1 , Humanos , Masculino , Fatores de RiscoRESUMO
It has been reported that UV-induced immunosuppression can be reversed by photoreactivation or exposure to T4 endonuclease V, two treatments that can repair cyclobutane pyrimidine dimers. These observations, together with the known role of urocanic acid (UA) in UV-induced immune suppression, prompted us to study the ability of DNA photolyase to repair UA-DNA cyclobutane photoadducts in single-stranded calf thymus DNA. We did not detect any release of UA, with a sensitivity implying that photolyase is at least 2900 times less active toward UA-DNA adducts than toward cis-syn thymine-thymine dimers. This indicates that any reversal of photoimmunosuppression by photoreactivation cannot significantly involve cleavage of UA-DNA cyclobutane adducts.
Assuntos
Adutos de DNA/metabolismo , Reparo do DNA , Ácido Urocânico/metabolismo , Animais , Bovinos , Ciclobutanos/metabolismo , Ciclobutanos/efeitos da radiação , Adutos de DNA/efeitos da radiação , Desoxirribodipirimidina Fotoliase/metabolismo , Técnicas In Vitro , Fotoquímica , Raios Ultravioleta/efeitos adversos , Ácido Urocânico/efeitos da radiaçãoRESUMO
The function of DNA polymerase II of Escherichia coli is an old question. Any phenotypic character that Pol II may confer upon the cell has escaped detection since the polymerase was discovered 24 yr ago. Although it has been shown that Pol II enables DNA synthesis to proceed past abasic sites in vitro, no role is known for it in the bypass of those lesions in vivo. From a study of phage S13 single-stranded DNA, we now report SOS conditions under which Pol II is needed for DNA synthesis to proceed past abasic sites with 100% efficiency in vivo. Overproduction of the GroES+L+ heat shock proteins, which are members of a ubiquitous family of molecular chaperones, eliminated this requirement for Pol II, which may explain why the role of Pol II in SOS repair had eluded discovery. Mutagenesis accompanied SOS bypass of abasic sites when the original occupant had been cytosine but not when it had been thymine; the quantitative difference is shown to imply that adenine was inserted opposite the abasic sites at least 99.7% of the time, which is an especially strict application of the A-rule. Most, but not all, spontaneous mutations from Rifs to Rifr, whether in a recA+ or a recA(Prtc) cell, require Pol II; while this suggests that cryptic abasic lesions are a likely source of spontaneous mutations, it also shows that such lesions cannot be the exclusive source.
Assuntos
Proteínas de Bactérias/metabolismo , DNA Polimerase II/metabolismo , DNA Bacteriano/biossíntese , Escherichia coli/enzimologia , Resposta SOS em Genética , Chaperonina 10 , Chaperonina 60 , Dano ao DNA , DNA Bacteriano/genética , DNA Bacteriano/efeitos da radiação , Proteínas de Choque Térmico/metabolismo , Mutagênese , Raios UltravioletaRESUMO
Naked, infectious single-stranded (ss) and double-stranded (ds) DNA from phages S13 and G4 were irradiated with 308 nm UV radiation in the absence and presence of several photobiologically active compounds: E- and Z-urocanic acid (E- and Z-UA), their methyl esters (E- and Z-MU), E- and Z-indoleacrylic acid (E- and Z-IA), cis-dichloro-bis(1,10-phenanthroline)rhodium(III) chloride (cDCBPR) and tris(1,10-phenanthroline)rhodium (III) perchlorate (TPR). E-urocanic acid protects against cyclobutane pyrimidine dimer formation in ssDNA but concomitantly photosensitizes the formation of other lesions that inactivate ssDNA. Z-urocanic acid also protects ssDNA against such dimerization but without the associated sensitized damage. The methyl ester isomers behave similarly. There is no such differential activity observed for the IA isomers, both of which sensitize the inactivation of ssDNA. Photostationary state mixtures of both UA and IA efficiently sensitize the inactivation of dsDNA, and cDCBPR strongly protects ssDNA from UV damage, while TPR is a significant sensitizer. Both of these metal complexes sensitize the inactivation of dsDNA slightly. For all compounds, cyclobutane pyrimidine dimers were the predominant lethal lesions produced by sensitization of the dsDNA, but they were not the major lethal lesions created by sensitization of the ssDNA. In the case of dsDNA, both UA and IA created pyrimidine dimers with a high degree of potential for mutagenesis, as determined by an assay that monitors the frequency of mutations following the spontaneous deamination of cytosine in photodimers.
Assuntos
DNA Viral/antagonistas & inibidores , DNA Viral/efeitos dos fármacos , Fármacos Fotossensibilizantes/farmacologia , Colífagos/efeitos dos fármacos , Colífagos/genética , Colífagos/efeitos da radiação , DNA de Cadeia Simples/antagonistas & inibidores , DNA de Cadeia Simples/efeitos dos fármacos , DNA de Cadeia Simples/efeitos da radiação , DNA Viral/efeitos da radiação , Indóis/farmacologia , Mutação/efeitos dos fármacos , Mutação/efeitos da radiação , Fotoquímica , Ródio/farmacologia , Ácido Urocânico/farmacologiaRESUMO
Mutagenesis studies have indicated that the deamination of cytosine in UV-induced cyclobutane pyrimidine dimers is a key part of an error-free process that can account for most of the C-->T base specificity that frequently characterizes UV-induced mutagenesis. The kinetics of deamination, as inferred from the mutagenic effect of delayed photoreactivation, is remarkable in its resemblance to a step function. To study the kinetics from a different point of view, we used an enzymatic approach combining photolyase and uracil-N-glycosylase treatment to detect the formation of uracil in UV-irradiated single or double-stranded infectious DNA of phage S13. Formation of abasic sites by removal of uracil was inferred from loss of infectivity. It is concluded that no uracil appeared at 37 degrees C within 20 minutes (single-stranded DNA) or 40 minutes (double-stranded DNA) after irradiation, but following those latent periods, most of the uracil residues rapidly appeared within a brief 14 minute period centered at 29 minutes (single-stranded DNA) and 55 minutes (double-stranded DNA) after irradiation. The timing appears to fully confirm the previous evidence that dimer cytosines in DNA deaminate with step-function-like kinetics. Furthermore, the appearance of uracil was shown to be dependent on the UV-induction of cyclobutane dimers containing cytosine.
Assuntos
Citosina/química , DNA de Cadeia Simples/química , DNA/química , Dímeros de Pirimidina/química , Uracila/química , Ácido Apurínico/química , Desaminação , CinéticaRESUMO
Twenty-eight recA mutants, isolated after spontaneous mutagenesis generated by the combined action of RecA1202(Prtc) and UmuDC proteins, were characterized and sequenced. The mutations are intragenic suppressors of the recA1202 allele and were detected by the reduced coprotease activity of the gene product. Twenty distinct mutation sites were found, among which two mutations, recA1620 (V-275-->D) and recA1631 (I-284-->N), were mapped in the C-terminal portion of the interfilament contact region (IFCR) in the RecA crystal. An interaction of this region with the part of the IFCR in which the recA1202 mutation (Q-184-->K) is mapped could occur only intermolecularly. Thus, altered IFCR and the likely resulting change in interfilament association appear to be important aspects of the formation of a constitutively active RecA coprotease. This observation is consistent with the filament-bundle theory (R. M. Story, I. T. Weber, and T. A. Steitz, Nature (London) 335:318-325, 1992). Furthermore, we found that among the 20 suppressor mutations, 3 missense mutations that lead to recombination-defective (Rec-) phenotypes also mapped in the IFCR, suggesting that the IFCR, with its putative function in interfilament association, is required for the recombinase activity of RecA. We propose that RecA-DNA complexes may form bundles analogous to the RecA bundles (lacking DNA) described by Story et al. and that these RecA-DNA bundles play a role in homologous recombination.
Assuntos
Recombinases Rec A/genética , Sequência de Aminoácidos , Sequência de Bases , Sítios de Ligação , Primers do DNA/química , Endopeptidases/genética , Escherichia coli/enzimologia , Substâncias Macromoleculares , Mitomicina/farmacologia , Dados de Sequência Molecular , Movimento (Física) , Mutagênese , Estrutura Terciária de Proteína , Recombinases Rec A/química , Recombinação Genética , Relação Estrutura-AtividadeRESUMO
We have shown that excision and transposition of Tn5 in Escherichia coli are greatly increased by recA(Prtc) genes, which encode constitutively activated RecA proteins (C.-T. Kuan, S.-K. Liu, and I. Tessman, Genetics 128:45-57, 1991). Contrary results, showing a significant decrease in Tn5 transposition under SOS conditions, were subsequently reported (M. D. Weinreich, J. C. Makris, and W. S. Reznikoff, J. Bacteriol. 173:6910-6918, 1991). We have extended our studies to examine the following: (i) transposition of Tn5 from sites in the phoA, phoB, proC, trpD, and ilvD genes; (ii) the effect of gene transcription; (iii) the comparative effect of dinD+ and dinD(Def) alleles; (iv) the use of a mating-out assay of transposition; (v) the effect of a recA(Prtc) allele located at the normal chromosomal site; and (vi) the effect at 41.5 degrees C of the recA441(Prtc) allele. The new results fully confirm our previous conclusions, including the fact that the frequency of Tn5 transposition under constitutive SOS conditions is site dependent.
Assuntos
Elementos de DNA Transponíveis/genética , Escherichia coli/genética , Genes Bacterianos/genética , Recombinases Rec A/genética , Transcrição Gênica , Alelos , Cruzamentos Genéticos , Fator F/genéticaRESUMO
We measured the kinetics of growth and mutagenesis of UV-irradiated DNA of phages S13 and lambda that were undergoing SOS repair; the kinetics strongly suggest that most of SOS mutagenesis arises from the deamination of cytosine in cyclobutane pyrimidine dimers, producing C----T transitions. This occurs because the SOS mechanism bypasses T--T dimers promptly, while bypass of cytosine-containing dimers is delayed long enough for deamination to occur. The mutations are thus primarily the product of a faithful mechanism of lesion bypass by a DNA polymerase and are not, as had been generally thought, the product of an error-prone mechanism. All of these observations are explained by the A-rule, which is that adenine nucleotides are inserted noninstructionally opposite DNA lesions.
Assuntos
Citosina/metabolismo , Dano ao DNA , DNA Viral/efeitos da radiação , Mutagênese , Resposta SOS em Genética , Escherichia coli/genética , Escherichia coli/efeitos da radiação , Dímeros de Pirimidina , Fatores de Tempo , Raios UltravioletaRESUMO
The LexA protein of Escherichia coli represses expression of a variety of genes that, by definition, constitute the SOS regulon. Genetic evidence suggests that Tn5 transposition is also regulated by the product of the lexA gene (C.-T. Kuan, S.-K. Liu, and I. Tessman, Genetics 128:45-57, 1991). We now show that the LexA protein represses expression of the tnp gene, located in the IS50R component of Tn5, which encodes a transposase, and that LexA does not repress expression of the IS50R inh gene, which encodes an inhibitor of transposition. Elimination of LexA resulted in increased expression of the tnp gene by a factor of 2.7 +/- 0.4, as indicated by the activity of a lacZ gene fused to the tnp gene. LexA protein retarded the electrophoretic movement of a 101-bp segment of IS50R DNA that contained a putative LexA protein-binding site in the tnp promoter; the interaction between the LexA repressor and the promoter region of the tnp gene appears to be relatively weak. These features show that the IS50R tnp gene is a member of the SOS regulon.
Assuntos
Proteínas de Bactérias/genética , Escherichia coli/genética , Regulação Bacteriana da Expressão Gênica/genética , Nucleotidiltransferases/genética , Serina Endopeptidases , Proteínas de Bactérias/metabolismo , Sequência de Bases , Elementos de DNA Transponíveis/genética , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Regiões Promotoras Genéticas/fisiologia , Proteínas Recombinantes de Fusão/biossíntese , Resposta SOS em Genética/genética , Transposases , beta-Galactosidase/genéticaRESUMO
Excision and transposition of the Tn5 element in Escherichia coli ordinarily appear to occur by recA-independent mechanisms. However, recA(Prtc) genes, which encode RecA proteins that are constitutively activated to the protease state, greatly enhanced excision and transposition; both events appeared to occur concomitantly and without destruction of the donor DNA. The recombinase function of the RecA protein was not required. Transposition was accompanied by partial, and occasionally full, restoration of the functional integrity of the gene vacated by the excised Tn5. The stimulation of transposition was inhibited by an uncleavable LexA protein and was strongly enhanced by an additional role of the RecA(Prtc) protein besides its mediation of LexA cleavage. To account for the enhanced transposition, we suggest that (i) there may be a LexA binding site within the promoter for the IS50 transposase, (ii) activated RecA may cleave the IS50 transposition inhibitor, and (iii) the transposase may be formed by RecA cleavage of a precursor molecule.
Assuntos
Elementos de DNA Transponíveis , Escherichia coli/genética , Resposta SOS em Genética , Serina Endopeptidases , Proteínas de Bactérias/metabolismo , Bacteriófago lambda/genética , Sequência de Bases , DNA Bacteriano , Genes Bacterianos , Dados de Sequência Molecular , Recombinases Rec A/metabolismo , Proteínas Repressoras/metabolismo , Mapeamento por RestriçãoRESUMO
A large increase in the incidence of bacteriophage mutants is found after photoreactivation of UV-irradiated phage S13. The increase was seen only when the irradiated phage were stored before they were photoreactivated; the maximum mutation frequency was achieved after storage for 2 h at 4 degrees C or 30 min at 37 degrees C. The mutations can be attributed entirely to deamination of cytosine in cyclobutane dimers. Naked S13 DNA was stored for 2 h at 37 degrees C after being irradiated with wavelengths greater than or equal to 290 nm in the presence of 0.2% acetophenone, which sensitizes the formation of thymine-thymine but not cytosine-containing dimers; the specific mutation frequency was 7.2-fold lower compared to the frequency produced by irradiation in the absence of the photosensitizer, confirming that cytosine dimers are a major source of mutations. These results undermine the basis for the two-step model of UV mutagenesis in which a distinctly separate misincorporation step is supposed to precede the lesion bypass step; instead the results support a different two-step model, in which a deamination step precedes the bypass. The S13 capsid appears to completely inhibit the putative deamination reaction at about 75% of the dimer sites.