Annular distribution patterns of .357 Magnum fragments in soft tissue simulants after striking hard material that prevented the bullet from exiting.

The distribution of bullet fragments inside the body can provide information for the reconstruction of events in shooting incidents. The formation of an annular distribution pattern of bullet fragments was recently presented in a case report. The fragments were scattered radially around an exit-re-entrance wound resulting from collision of the bullet with a floor tile immediately after perforating the body. Such an annular distribution pattern of bullet fragments around an exit-re-entrance wound would indicate that a body was in close contact with hard material, for instance, lying on hard ground or leaning against a concrete wall, when the shot was fired. The aim of this experimental study was to investigate the formation and reproducibility of the annular distribution pattern of bullet fragments. It was assumed that the distribution pattern would be formed when hard material blocks a bullet from exiting a soft tissue simulant. Furthermore, the dependency of this distribution pattern on the impact angle was assessed. For this purpose, .357 Magnum bullets were fired at ballistic soap blocks with a steel plate at the rear end of the soap block. Six shots were performed at an impact angle of 90° (experiment 1), and six shots were performed at an impact angle of 45° (experiment 2). The distribution pattern of the fragments inside the individual soap blocks was examined via computed tomography (CT). In experiment 1, the bullets burst, and large fragments formed annular distribution patterns with a radial extent of approximately 4.9 cm and a maximum depth of approximately 2.3 cm. In experiment 2, the bullets ricocheted from the steel plate, and tiny fragments formed small annular distribution patterns at the points of ricochet with a radial extent of approximately 1.5 cm and a maximum depth of approximately 1.2 cm. The end position of the large main fragments was approximately 9.7 cm distant from the point of ricochet at a mean depth of 2.7 cm. The mean kinetic energy of the bullets at the time of impact was 580 J in experiment 1 and 394 J in experiment 2. Distribution patterns of bullet fragments in the body may provide information not only on the impact angle of a bullet but also on whether the body was in contact with a hard material that blocked the bullet from exiting the body. CT proved to be an appropriate imaging method for such investigations.

Evaluation of an acetated Ringer-based contrast material mixture for postmortem computed tomography angiography.

PURPOSE: The purpose of this study was to compare an established postmortem contrast medium mixture based on polyethylene glycol (PEG) to an isotonic crystalloid with acetated Ringer solution (AR) as the base, both mixed with water-soluble iodinated contrast medium for postmortem computed tomography angiography (PMCTA) with the aim to avoid alterations of the corpse during autopsy. MATERIALS AND METHODS: The study included 20 cadavers; 10 had PMCTA with AR and 10 with PEG. PMCTA images were analyzed with respect to image quality, vascular contrast patterns and artifacts. Autopsy was evaluated for visual, organ, vessel and haptic alterations. The Wilcoxon rank sum test was used to search for differences in image quality between the two groups. Statistical significance was set at P<0.05. RESULTS: AR provided excellent contrast within the right coronary artery (P<0.001) but a lack of contrast within the left coronary artery (P=0.008) whereas PEG showed the opposite. A better image quality was observed in the PEG group by comparison with the AR group for right common carotid artery (P=0.03), left common carotid artery (P=0.01) and left coronary artery (P=0.008). No differences were found for ascending aorta (P=0.65), aortic arch (P=0.09), right circle of Willis (P=0.17), left circle of Willis (P=0.08), inferior vena cava (P=0.07) and abdominal aorta (P=0.08). Severe extravasation occurred in all (10/10; 100%) cadavers in the AR group but in none (0/10; 0%) in the PEG group (P<0.001). At autopsy, visual alteration with lilac discoloration of the face was observed in 4/10 cadavers (40%) in the AR group and in 9/10 cadavers (90%) in the PEG group (P=0.057). Haptic alterations were observed in 3/10 cadavers (30%) in the AR group and 10/10 cadavers (100%) in the PEG group (P=0.003). CONCLUSION: AR results in contrast medium mixture extravasation in all cadavers, but PEG altered the autopsy more severely. Both carrier substances result in specific substance-related artifacts and dependent opacification of the coronary arteries, but PEG is recommended for PMCTA exclusively with regard to diagnostic imaging.

Fracture energy threshold in parry injuries due to sharp and blunt force.

Blows with axes, machetes or blunt objects such as baseball bats, truncheons, etc. are often parried, resulting in typical parry injuries, or so-called nightstick fractures to the ulna. In this study, we sought to assess the impact energy required to break the ulna in such parry incidents in an experimental setting using semisynthetic and fully synthetic models. Twenty-seven sheep radii and 33 polyurethane synthetic bones were cast into gelatin prior to being fired at with missiles made of a section of an axe blade or steel rod at different firing velocities using a compressed-nitrogen cannon. Each model was then examined as to the presence of hair-line fractures or complete fractures. Sheep bones and synthetic bones displayed comparable results when struck by the axe missile; here, a clear fracture threshold was evident between 14.00 and 15.26 J. When struck by the rod missile, only the synthetic bones produced significant results, namely a fracture threshold between 20.15 and 23.59 J. In conclusion, our results show an ulnar fracture threshold of approximately 15 J when struck by an axe. The experiments regarding blows with a rod displayed a fracture threshold of around 22 J, but, as this could not be validated with biological bones, this result is questionable.

Estimating the postmortem interval of human skeletal remains by analyzing their fluorescence at 365 and 490 nm.

The study presented here is regarded as a continuation of the experiments of Sterzik et al., who developed a new practical method to distinguish between historical and recent human skeletal remains. Bone cross-sections were illuminated using light with wavelengths of 365 and 490 nm, causing fluorescence. The fluorescence was documented by photography and further analyzed to examine the areal extent of a certain fluorescent color. Contrary to the previous experiments of Sterzik et al., the present study focused on bones with postmortem intervals (PMIs) ≥ 50 years. Therefore, this study fills the gap created by the former study, pointing towards a correlation between the PMI and the areal extent of the fluorescent surface in both tests. The presence of blue and red fluorescent surfaces < 1% indicated a PMI ≥ 50 years. Furthermore, the presence of blue and red fluorescent surfaces > 1% can be regarded as a marker to exclude a PMI ≥ 50 years; in fact, these bones are likely to have a PMI < 30 years.

A fully synthetic lung model for wound-ballistic experiments-First results.

PURPOSE: Today, synthetic models have all but replaced animal and corpse models in examining damage to soft-tissues and skeletal structures by ballistic trauma. As, however, non-solid organs such as the lungs, have not been able to be replaced by a fully synthetic model we attempted to create such a model. MATERIAL AND METHODS: 20% ordnance gelatine was frothed with a household mixer and cooled to stable foam. Several of these foam blocks were then stuck together with liquid gelatine and placed between 10% gelatine blocks. As controls, we embedded pig lungs in gelatine and compared the wound channels seen in computed tomography created upon shooting with 9mm Luger. RESULTS: The fully synthetic models displayed radiological and physical densities comparable to real lungs. The wound profile characteristics of the fully synthetic lung models were very similar to the semisynthetic swine-gelatine models regarding the permanent wound cavity. Furthermore, in both semi- and fully synthetic models we detected a ring surrounding the permanent wound channel, most likely representing the remnants of the temporary wound cavity. CONCLUSION: Our results indicate that this fully synthetic lung model is a viable substitute for ballistic experiments on lungs. We believe that further research on the temporary wound channel in lungs is possible with this model in order to provide more insight into the effect of ballistic trauma to the lungs not seen otherwise.

"Blind spots" in forensic autopsy: improved detection of retrobulbar hemorrhage and orbital lesions by postmortem computed tomography (PMCT).

OBJECTIVES: The purpose of this study was to correlate the occurrence of retrobulbar hemorrhage (RBH) with mechanism of injury, external signs and autopsy findings to postmortem computed tomography (PMCT). METHODS: Six-teen subjects presented with RBH and underwent PMCT, external inspection and conventional autopsy. External inspection was evaluated for findings of the bulbs, black eye, raccoon eyes and Battle's sign. Fractures of the viscerocranium, orbital lesions and RBH were evaluated by PMCT. Autopsy and PMCT was evaluated for orbital roof and basilar skull fracture. RESULTS: The leading manner of death was accident with central regulatory failure in cases of RBH (31.25%). Imaging showed a high sensitivity in detection of orbital roof and basilar skull fractures (100%), but was less specific compared to autopsy. Volume of RBH (0.1-2.4ml) correlated positively to the presence of Battle's sign (p<0.06) and the postmortem interval. Ecchymosis on external inspection correlated with RBH. There was a statistical significant correlation between bulbar lesion and RBH. Orbital roof fracture count weakly correlated with the total PMCT derived RBH volume. Maxillary hemosinus correlated to maxillary fractures, but not to RBH. CONCLUSIONS: RBH are a specific finding in forensically relevant head trauma. PMCT is an excellent tool in detecting and quantifying morphological trauma findings particularly in the viscerocranium, one of the most relevant "blind spots" of classic autopsy. PMCT was superior in detecting osseous lesions, scrutinizing autopsy as the gold standard.

CT metal artefact reduction for internal fixation of the proximal humerus: value of mono-energetic extrapolation from dual-energy and iterative reconstructions.

AIM: To assess the value of dual-energy computed tomography (DECT) and an iterative frequency split-normalized metal artefact reduction (IFS-MAR) algorithm compared to filtered back projections (FBP) from single-energy CT (SECT) for artefact reduction in internally fixated humeral fractures. MATERIALS AND METHODS: Six internally fixated cadaveric humeri were examined using SECT and DECT. Data were reconstructed using FBP, IFS-MAR, and mono-energetic DECT extrapolations. Image analysis included radiodensity values and qualitative evaluation of artefacts, image quality, and level of confidence for localizing screw tips. RESULTS: Radiodensity values of streak artefacts were significantly different (p < 0.05) between FBP (-104 ± 222) and IFS-MAR (73 ± 122), and between FBP and DECT (32 ± 151), without differences between IFS-MAR and DECT (p < 0.553). Compared to FBP, qualitative artefacts were significantly reduced using IFS-MAR (p < 0.001) and DECT (p < 0.05), without significant differences between IFS-MAR and DECT (p < 0.219). Image quality significantly (p = 0.016) improved for IFS-MAR and DECT compared to FBP, without significant differences between IFS-MAR and DECT (p < 0.553). The level of confidence for screw tip localization was assessed as best for DECT in all cases. CONCLUSION: Both IFS-MAR in SECT and mono-energetic DECT produce improved image quality and a reduction of metal artefacts. Screw tip positions can be most confidently assessed using DECT.

Walking on thin ice! Identifying methamphetamine "drug mules" on digital plain radiography.

OBJECTIVE: The purpose of this study was to retrospectively evaluate the sensitivity, specificity and accuracy of identifying methamphetamine (MA) internal payloads in "drug mules" by plain abdominal digital radiography (DR). METHODS: The study consisted of 35 individuals suspected of internal MA drug containers. A total of 59 supine digital radiographs were collected. An overall calculation regarding the diagnostic accuracy for all "drug mules" and a specific evaluation concerning the radiological appearance of drug packs as well as the rate of clearance and complications in correlation with the reader's experience were performed. The gold standard was the presence of secured drug packs in the faeces. RESULTS: There were 16 true-positive "drug mules" identified. DR of all drug carriers for Group 1 (forensic imaging experienced readers, n = 2) exhibited a sensitivity of 100%, a mean specificity of 76.3%, positive predictive value (PPV) of 78.5%, negative predictive value (NPV) of 100% and a mean accuracy 87.2%. Group 2 (inexperienced readers, n = 3) showed a lower sensitivity (93.7%), a mean specificity of 86%, a PPV of 86.5%, an NPV of 94.1% and a mean accuracy of 89.5%. The interrater agreement within Group 1 was 0.72 and within Group 2 averaged to 0.79, indicating a fair to very good agreement. CONCLUSION: DR is a valuable screening tool in cases of MA body packers with huge internal payloads being associated with a high diagnostic insecurity. Diagnostic insecurity on plain films may be overcome by low-dose CT as a cross-sectional imaging modality and addressed by improved radiological education in reporting drug carriers on imaging. ADVANCES IN KNOWLEDGE: Diagnostic signs (double-condom and halo signs) on digital plain radiography are specific in MA "drug mules", although DR is associated with high diagnostic insecurity and underreports the total internal payload.

Essentials of forensic post-mortem MR imaging in adults.

Post-mortem MR (PMMR) imaging is a powerful diagnostic tool with a wide scope in forensic radiology. In the past 20 years, PMMR has been used as both an adjunct and an alternative to autopsy. The role of PMMR in forensic death investigations largely depends on the rules and habits of local jurisdictions, availability of experts, financial resources, and individual case circumstances. PMMR images are affected by post-mortem changes, including position-dependent sedimentation, variable body temperature and decomposition. Investigators must be familiar with the appearance of normal findings on PMMR to distinguish them from disease or injury. Coronal whole-body images provide a comprehensive overview. Notably, short tau inversion-recovery (STIR) images enable investigators to screen for pathological fluid accumulation, to which we refer as "forensic sentinel sign". If scan time is short, subsequent PMMR imaging may be focussed on regions with a positive forensic sentinel sign. PMMR offers excellent anatomical detail and is especially useful to visualize pathologies of the brain, heart, subcutaneous fat tissue and abdominal organs. PMMR may also be used to document skeletal injury. Cardiovascular imaging is a core area of PMMR imaging and growing evidence indicates that PMMR is able to detect ischaemic injury at an earlier stage than traditional autopsy and routine histology. The aim of this review is to present an overview of normal findings on forensic PMMR, provide general advice on the application of PMMR and summarise the current literature on PMMR imaging of the head and neck, cardiovascular system, abdomen and musculoskeletal system.

Pilot study on doctors working in departments of forensic medicine in German-speaking areas.

Several directors of institutes of forensic and legal medicine in German-speaking areas have noticed a lack of young doctors with specialty qualifications (full board certification) in forensic medicine during recent years. The pilot study was intended to brainstorm the possible reasons for this shortage, by carrying out a survey of doctors working in departments of forensic medicine, paying particular attention to job satisfaction and opinions as to why there are fewer forensic specialists. We sent the link to an online questionnaire to all members of the societies of forensic medicine in Germany, Switzerland and Austria. Altogether, 129 respondents completed the questionnaire and were included in the study. Slightly more men than women replied; the mean age of all respondents was 41. Most respondents had completed their specialty training and worked full-time. In general, participants were moderately satisfied with their careers. Men reported greater career success than women, as determined by objective criteria. Career support was considered to be suboptimal. For most of the respondents, the level of enjoyment of working in forensic medicine was either higher than or approximately the same as the level recalled from five years earlier. Possible reasons for the lack of qualified doctors in forensic medicine institutes are the non-availability of both senior posts and specialty training posts. Career opportunities in forensic medicine are not considered to be attractive.

Temperature-induced sex reversal is not responsible for sex-ratio distortions in grayling Thymallus thymallus or brown trout Salmo trutta.

On the basis of the experiments carried out over various years, it was concluded that (1) grayling Thymallus thymallus and brown trout Salmo trutta are resistant to temperature-induced sex reversal at ecologically relevant temperatures, (2) environmental sex reversal is unlikely to cause the persistent sex ratio distortion observed in at least one of the study populations and (3) sex-specific tolerance of temperature-related stress may be the cause of distorted sex ratios in populations of T. thymallus or S. trutta.

Sudden death in a case of sickle cell anemia: post-mortem computed tomography and autopsy correlation from a radiologist's perspective.

Sickle cell anemia (SCA) is a hemolytic disease characterized by the production of abnormal hemoglobin chains and distorted red blood cell morphology or sickling. "Sickle cell crisis" includes vaso-occlusive crisis, a plastic crisis, sequestration crisis, haemolytic crisis and often culminating in serious complications, organ damage and even sudden death. Post-mortem computed tomography (PMCT) findings of sickle cell disease have never been reported in literature. This case of sudden death from acute hemolytic crisis in SCA where post-mortem computed tomography (PMCT) and autopsy findings complemented each other, both revealing findings invisible to the other and both crucial to the case.

[Virtual autopsy (virtopsy) in forensic science: from the scalpel to the scanner]. / Virtuelle Autopsie (Virtopsy) in der Forensik: Vom Skalpell zum Scanner.

The aim of "virtopsy" is to utilize modern imaging technology to optimize classical autopsy documentation. The benefits of virtopsy include examiner-independent, objective 3D documentation and its non-invasive approach. Virtopsy is an option in cases where autopsy is rejected by family members or for religious reasons. It is also suitable as a rapid identification and examination tool in large-scale disasters. Forensic findings can be presented to prosecutors and courts in 3D and without bloody images.

Differentiation of ante-mortem and post-mortem fractures with MRI: a case report.

We describe a case of a fatal speed flying accident in which the victim was electrocuted, burned and fell from a great height. Post-mortem imaging revealed acute appearing fractures on CT, without bone marrow oedema on MRI. Based on the known clinical imaging findings of bone marrow oedema in acute fractures, we concluded that the speed flyer died from electrocution rather than the fall and that the fractures occurred post-mortem. Radiological imaging augmented the reconstruction of the peri-mortem events. Further research is needed to assess whether bone marrow oedema in acute fractures is a reliable vital sign.

Radiological stab wound channel depiction with instillation of contrast medium.

The radiological depiction of stab wounds in soft-tissues using multislice computed tomography is difficult and the hereby obtained images often lack contrast. To overcome these shortcomings we tried a new method. We gently instilled the contrast medium Lipiodol((R)) Ultra-fluide into five experimentally induced stab wounds on a pork haunch. Subsequent MSCT reconstructions of the wounds delivered clear, for medical laymen easily appreciable images regarding the stab direction and the stab depth. We believe that this easy and rapid technique can be useful in the examination of stab wounds in living and dead victims of sharp trauma.

Post-mortem imaging of laryngohyoid fractures in strangulation incidents: first results.

Fractures and soft-tissue injuries of the neck are of great importance in forensic pathology, as they help in assessing whether strangulation took place, and if so, how severely. In this study, we examined the usefulness of post-mortem imaging with multislice computed tomography (MSCT) in detecting lesions of the laryngohyoid structures and the surrounding soft-tissues. For this purpose, we examined MSCT images of the neck of eight deceased persons who had suffered different types of strangulation and compared the findings with those obtained at the subsequent forensic autopsy. In six of the eight cases (75%), the fracture findings at autopsy were concordant with those found with MSCT. In the two non-congruent cases, MSCT revealed fractures, which were not discovered at autopsy. Soft-tissue haemorrhages were detected by autopsy in five cases, but only in one case with MSCT. MSCT does not suffice in detecting soft-tissue injuries. These preliminary results are promising regarding the detection of fractures in strangulation cases. If these results can be confirmed in larger studies, we believe that post-mortem MSCT may serve - in combination with a thorough external examination and a profound incident-scene investigation - as a useful decision-making tool regarding the necessity of further examinations, i.e. autopsy.

Incidence of auditory ossicle luxation and petrous bone fractures detected in post-mortem multislice computed tomography (MSCT).

As the auditory ossicles are difficult to display without harming them in conventional autopsies, lesions of these minute bones and the ossicular chain are regularly missed. In this study, the method of choice in clinical medicine for the examination of such lesions, namely multislice computed tomography, was applied to 100 corpses. The hereby obtained results regarding ossicle luxation and petrous bone fracture indicated that the lesions were not dependant on the amount, but rather on the type of energy inflicted to the head.

Injuries due to sharp trauma detected by post-mortem multislice computed tomography (MSCT): a feasibility study.

Modern cross-sectional imaging techniques are being increasingly implemented in forensic pathology. In order to assess the practicability of such a method, namely post-mortem multislice computed tomography (MSCT) in cases of fatal cut and stab injuries, 12 corpses underwent such an examination prior to forensic autopsy. The questions regarding detection of foreign bodies, wound channels, skeletal and organ injuries, as well as the cause of death were addressed at MSCT and autopsy. The results of the two techniques revealed that post-mortem MSCT a useful tool in the assessment of such injuries.