RESUMO
A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based ELISA could be used as an alternative to MAT for the diagnosis of bovine leptospirosis.
Assuntos
Antígenos de Bactérias/química , Proteínas da Membrana Bacteriana Externa/química , Doenças dos Bovinos/microbiologia , Leptospira interrogans/isolamento & purificação , Leptospirose/veterinária , Lipoproteínas/química , Animais , Anticorpos Antibacterianos/sangue , Antígenos de Bactérias/biossíntese , Antígenos de Bactérias/genética , Proteínas da Membrana Bacteriana Externa/biossíntese , Proteínas da Membrana Bacteriana Externa/genética , Bovinos , Doenças dos Bovinos/sangue , Ensaio de Imunoadsorção Enzimática/veterinária , Leptospirose/sangue , Leptospirose/microbiologia , Lipoproteínas/biossíntese , Lipoproteínas/genética , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Sensibilidade e EspecificidadeAssuntos
Proteínas da Membrana Bacteriana Externa/genética , Leptospira interrogans serovar canicola/genética , Sequência de Aminoácidos , Proteínas da Membrana Bacteriana Externa/química , Sequência de Bases , Clonagem Molecular , Regulação Bacteriana da Expressão Gênica , Dados de Sequência Molecular , FilogeniaAssuntos
Antígenos de Bactérias , Proteínas da Membrana Bacteriana Externa , Doenças dos Bovinos/diagnóstico , Leptospira interrogans serovar canicola/imunologia , Leptospirose/veterinária , Proteínas Recombinantes , Animais , Antígenos de Bactérias/imunologia , Proteínas da Membrana Bacteriana Externa/imunologia , Bovinos , Doenças dos Bovinos/microbiologia , Leptospirose/diagnóstico , Lipoproteínas/imunologia , Coelhos , Sensibilidade e EspecificidadeRESUMO
The efficacy of a recombinant leptospiral lipoprotein LipL41 as an antigen for conducting enzyme-linked immunosorbent assay (ELISA) for diagnosis of bovine leptospirosis was evaluated. Using known positive and known negative cattle sera the recombinant antigen was found to be highly reactive in the concentration of 100 ng/well. Using a total of 321 field cattle sera the sensitivity of ELISA as compared to microscopic agglutination test (MAT) was calculated to be 100% whereas the specificity was 85.3%. The seropositivity of leptospirosis among bovine population was found to be 21.18% having the predominance of serovars Sejroe and Pomona. It was concluded that rLipL41 protein could be a putative diagnostic candidate for serodiagnosis of bovine leptospirosis.
