RESUMO
A first case of subcutaneous infection caused by Saksenaea vasiformis in an 11-year-old Thai thalassaemic boy was described. The specific diagnosis of infection was attained from microscopic demonstration of the causal agent as well as the isolation of the fungus. The patient was successfully treated by debridement of the lesion and parenteral amphotericin B. The predisposing factors, source of infection and regimen for treatment are discussed.
Assuntos
Dermatomicoses/etiologia , Mucorales/isolamento & purificação , Mucormicose/etiologia , Talassemia/complicações , Criança , Humanos , Masculino , TailândiaRESUMO
Three human isolates of Pythium insidiosum De Cock 1987, the aetiologic agent of pythiosis, were induced for zoosporogenesis. The isolates were grown on Sabouraud glucose agar for 2 days before being inoculated into boiled spikelets of Axonopus compressus (Gramineae) and then into induction medium (IM). Optimum zoospore formation was demonstrated under the light microscope after incubation for 24 h at 37 degrees C in IM.
Assuntos
Pythium/fisiologia , Meios de Cultura , Humanos , Esporos FúngicosRESUMO
Aflatoxin-producing fungi were found in fermented foods and beverages: fermented rice (kaomak), soybean sauce (taotjo), peanut butter, soy sauce (shoyu), Thai red and white wine, and rice sugar wine. These foods were extracted directly and tested for aflatoxins by thin-layer chromatography (TLC) and high pressure liquid chromatography (HPLC). Four strains of aflatoxin-producing fungi were isolated from peanut butter, taotjo, and shoyu. Direct extracts of 10% of the peanut butters tested and 5% of the kaomak tested contained large amounts of aflatoxins. The HPLC procedures used in this experiment utilized chloroform-ethyl acetate (3:1).