Hedgehog signaling regulates the development and treatment of glioblastoma.

Glioblastoma (GBM) is the most common and fatal malignant tumor type of the central nervous system. GBM affects public health and it is important to identify biomarkers to improve diagnosis, reduce drug resistance and improve prognosis (e.g., personalized targeted therapies). Hedgehog (HH) signaling has an important role in embryonic development, tissue regeneration and stem cell renewal. A large amount of evidence indicates that both normative and non-normative HH signals have an important role in GBM. The present study reviewed the role of the HH signaling pathway in the occurrence and progression of GBM. Furthermore, the effectiveness of drugs that target different components of the HH pathway was also examined. The HH pathway has an important role in reversing drug resistance after GBM conventional treatment. The present review highlighted the relevance of HH signaling in GBM and outlined that this pathway has a key role in the occurrence, development and treatment of GBM.

Pseudo-random modulation continuous-wave lidar for the measurements of mesopause region sodium density.

The sodium fluorescence lidar utilizes a 589 nm narrowband pulse laser system to measure mesopause region atomic sodium density, atmospheric temperature, and wind. However, this system is complicated and unstable. The continuous-wave (CW) sodium laser system can achieve ultra-narrow bandwidth, all-solid-state, and small compact size, as such it is extremely valuable for mobile, aircraft, and space-borne applications. In this study, we developed the first pseudo-random modulated CW (PMCW) sodium lidar by using an electro-optic modulated narrowband 589 nm CW laser with an output power of ∼1.2W. A pseudorandom M-sequence-code with a length of 127 is used to achieve altitude information by modulating laser and then decoding photon signals. Also, a biaxial structure with 9 m separation between the optical axes of the transmitter and receiver is designed to suppress the strong near-ground signals, which are crucial for improving the signal-to-noise ratio (SNR) of the PMCW lidar system. Nighttime measurements on December 2-4, 2019 show that the SNR at sodium layer peak is more than 10, corresponding to a statistical uncertainty of less than 10% in sodium density with temporal and spatial resolutions of 5 min and 1.05 km respectively. The comparison of vertical profiles of sodium density simultaneously observed by PMCW lidar and collocated pulse lidar shows good agreement.

A mobile differential absorption lidar for simultaneous observations of tropospheric and stratospheric ozone over Tibet.

We developed a mobile ozone differential absorption lidar system to simultaneously measure the vertical profiles of tropospheric and stratospheric ozone from an altitude of ~5 to 50 km. The system emits four laser beams at wavelength of 289 nm, 299 nm, 308 nm and 355 nm and receives their corresponding Mie/Rayleigh backscattering return signals, and two N2 Raman return signals at 332 nm and 387 nm shifted from 308 nm and 355 nm, respectively. An assembled telescope array with four 1.25-m telescopes (effective diameter > 2 m) collects the Rayleigh and Raman backscattering signals at 308/332 and 355/387 nm. This system is currently deployed at the Yangbajing Observatory in Tibet (~4300 m elevation) and has begun observations in regular campaign mode since October 2017. The lidar results agree very well with those observed by the Aura/MLS satellite. This novel ozone lidar system operates at the highest elevation of any such system in the world. The higher elevation and larger receiver aperture of this system yield a higher signal-to-noise ratio and lower statistical uncertainty.

Activation of HO-1 protects placental cells function in oxidative stress via regulating ZO-1/occludin.

We previously confirmed that Nuclear factor erythroid 2-related factor-2 (Nrf2) and heme oxygenase (HO-1) play synergistic roles in the pathogenesis of preeclampsia. To further explore the function of HO-1 in the pathogenesis of preeclampsia, we established oxidative stress models respectively with human first-trimester trophoblast/simian virus (HTR8/SVneo) and human umbilical vein endothelial cells (HUVECs) and then assessed the effect of HO-1 on the two cell lines in oxidative stress conditions. The cell oxidative stress models were incubated with Hemin (an inducer of HO-1), then, the HTR8/SVneo cells were transfected by ZO-1 small interfering RNA (siRNA). The HTR-8/SVneo invasive abilities were detected, and the tube formation abilities of HUVECs were measured. HO-1 and tight junction proteins zonula occludens-1 (ZO-1) and occludin in the cells were detected. In both the trophoblastic and HUVEC oxidative stress models, HO-1、ZO-1 and occludin were increased incubated with Hemin. Meanwhile, HTR-8/SVneo cells incubated with Hemin showed increased invasion function against the destruction of hydrogen peroxide (H2O2). Similarly, the tube formation ability of HUVECs incubated with Hemin was increased. The above-mentioned effects were disappeared after HTR-8/SVneo cells were transfected by ZO-1 siRNA. These results suggest that HO-1 protects the function of placental cells in oxidative stress via regulating ZO-1/occludin.