Soil acidification occurs under ambient conditions but is retarded by repeated drought: results of a field-scale climate manipulation experiment.

Acid atmospheric emissions within Europe and North America have decreased strongly since 1985 and most recent acidification studies have focused on the changes occurring within ecosystems as a result of this decreased deposition. This current study documents a soil acidification trend under ambient N deposition conditions over a 13 year period, suggesting that acidification continues to be a process of concern at this Calluna vulgaris dominated heathland with an acidic sandy soil. The annual manipulation of climatic conditions on this heathland simulated the predicted summer rainfall reduction (drought) and resulted in a long term retardation of the soil acidification trend. The pH of the soil solution significantly decreased over the course of the trial for both treatments, however, in the final 2 years the decline continued only in the Control treatment. This retardation is primarily associated with the reduction in rainfall leading to lower drainage rates, reduced loss of cations and therefore reduced lowering of the soil acid neutralizing capacity (ANC). However, a change in the underlying mechanisms also indicated that N transformations became less important in the Drought treatment. This change corresponded to an increase in groundcover of an air-pollution tolerant moss species and it is hypothesized that this increasing moss cover filtered an increasing quantity of deposited N, thus reducing the N available for transformation. A soil acidification lag time is expected to increase between the two treatments due to the cumulative disparity in cation retention and rates of proton formation. To the authors' knowledge, this is the first study in which such acidification trends have been demonstrated in a field-scale climate manipulation experiment.

Sinks for nitrogen inputs in terrestrial ecosystems: a meta-analysis of 15N tracer field studies.

Effects of anthropogenic nitrogen (N) deposition and the ability of terrestrial ecosystems to store carbon (C) depend in part on the amount of N retained in the system and its partitioning among plant and soil pools. We conducted a meta-analysis of studies at 48 sites across four continents that used enriched 15N isotope tracers in order to synthesize information about total ecosystem N retention (i.e., total ecosystem 15N recovery in plant and soil pools) across natural systems and N partitioning among ecosystem pools. The greatest recoveries of ecosystem 15N tracer occurred in shrublands (mean, 89.5%) and wetlands (84.8%) followed by forests (74.9%) and grasslands (51.8%). In the short term (< 1 week after 15N tracer application), total ecosystem 15N recovery was negatively correlated with fine-root and soil 15N natural abundance, and organic soil C and N concentration but was positively correlated with mean annual temperature and mineral soil C:N. In the longer term (3-18 months after 15N tracer application), total ecosystem 15N retention was negatively correlated with foliar natural-abundance 15N but was positively correlated with mineral soil C and N concentration and C:N, showing that plant and soil natural-abundance 15N and soil C:N are good indicators of total ecosystem N retention. Foliar N concentration was not significantly related to ecosystem 15N tracer recovery, suggesting that plant N status is not a good predictor of total ecosystem N retention. Because the largest ecosystem sinks for 15N tracer were below ground in forests, shrublands, and grasslands, we conclude that growth enhancement and potential for increased C storage in aboveground biomass from atmospheric N deposition is likely to be modest in these ecosystems. Total ecosystem 15N recovery decreased with N fertilization, with an apparent threshold fertilization rate of 46 kg N x ha(-1) x yr(-1) above which most ecosystems showed net losses of applied 15N tracer in response to N fertilizer addition.

Carbon and nitrogen cycles in European ecosystems respond differently to global warming.

The global climate is predicted to become significantly warmer over the next century. This will affect ecosystem processes and the functioning of semi natural and natural ecosystems in many parts of the world. However, as various ecosystem processes may be affected to a different extent, balances between different ecosystem processes as well as between different ecosystems may shift and lead to major unpredicted changes. In this study four European shrubland ecosystems along a north-south temperature gradient were experimentally warmed by a novel nighttime warming technique. Biogeochemical cycling of both carbon and nitrogen was affected at the colder sites with increased carbon uptake for plant growth as well as increased carbon loss through soil respiration. Carbon uptake by plant growth was more sensitive to warming than expected from the temperature response across the sites while carbon loss through soil respiration reacted to warming in agreement with the overall Q10 and response functions to temperature across the sites. Opposite to carbon, the nitrogen mineralization was relatively insensitive to the temperature increase and was mainly affected by changes in soil moisture. The results suggest that C and N cycles respond asymmetrically to warming, which may lead to progressive nitrogen limitation and thereby acclimation in plant production. This further suggests that in many temperate zones nitrogen deposition has to be accounted for, not only with respect to the impact on water quality through increased nitrogen leaching where N deposition is high, but also in predictions of carbon sequestration in terrestrial ecosystems under future climatic conditions. Finally the results indicate that on the short term the above-ground processes are more sensitive to temperature changes than the below ground processes.

Root-surface phosphatase activity in shrublands across a European gradient: effects of warming.

Root-surface phosphatase activities were measured in natural and semi-natural shrublands across an European climatic gradient of temperature and rainfall including Wales (WL), Denmark (DK), Netherlands (NL), Hungary (HU), Italy (IT) and Spain (SP). In each site a warming experiment was conducted since 1999 or 2001 by means of passive night-time warming using reflective curtains that covered the vegetation at night. The treatments increased yearly average soil temperatures around 0. 8 degrees C in most of sites. Root-surface phosphatase activity values ranged between 56 mg PNP g(-1) h(-1) in IT and 3.5 mg PNP g(-1) h(-1) in HU. Warming had no effect on root-surface phosphatase activity across the sites and only in Hungary a slight increase was detected. Plants at Mediterranean sites (IT, SP) showed a higher root-surface phosphatase activity than plants at temperate sites (WL, NL, DK). We suggest it might be an adaptation of plant species evolved under Mediterranean climate that allows them a) to compensate in wet period for the decrease in phosphatase activity, and thus P uptake, during drought periods, and/or b) to benefit from soluble organic P flushes following the frequent drying-rewetting episodes experienced by soils in Mediterranean ecosystems.

Challenges in quantifying biosphere-atmosphere exchange of nitrogen species.

Recent research in nitrogen exchange with the atmosphere has separated research communities according to N form. The integrated perspective needed to quantify the net effect of N on greenhouse-gas balance is being addressed by the NitroEurope Integrated Project (NEU). Recent advances have depended on improved methodologies, while ongoing challenges include gas-aerosol interactions, organic nitrogen and N(2) fluxes. The NEU strategy applies a 3-tier Flux Network together with a Manipulation Network of global-change experiments, linked by common protocols to facilitate model application. Substantial progress has been made in modelling N fluxes, especially for N(2)O, NO and bi-directional NH(3) exchange. Landscape analysis represents an emerging challenge to address the spatial interactions between farms, fields, ecosystems, catchments and air dispersion/deposition. European up-scaling of N fluxes is highly uncertain and a key priority is for better data on agricultural practices. Finally, attention is needed to develop N flux verification procedures to assess compliance with international protocols.

Natural 15N abundance in two nitrogen saturated forest ecosystems.

Natural 15N abundance values were measured in needles, twigs, wood, soil, bulk precipitation, throughfall and soil water in a Douglas fir (Pseudotsuga menziesii (Mirb.) and a Scots pine (Pinus sylvestris L.) stand receiving high loads of nitrogen in throughfall (>50 kg N ha-1 year-1). In the Douglas fir stand δ15N values of the vegetation ranged between -5.7 and -4.2‰ with little variation between different compartments. The vegetation of the Scots pine stand was less depleted in 15N and varied from -3.3 to -1.2‰Î´15N. At both sites δ15N values increased with soil depth, from -5.7‰ and -1.2‰ in the organic layer to +4.1‰ and +4.7‰ at 70 cm soil depth in the Douglas fir and Scots pine stand, respectively. The δ15N values of inorganic nitrogen in bulk precipitation showed a seasonal variation with a mean in NH4+-N of -0.6‰ at the Douglas fir stand and +10.8‰ at the Scots pine stand. In soil water below the organic layer NH4+-N was enriched and NO3--N depleted in 15N, which was interpreted as being caused by isotope fractionation accompanying high nitrification rates in the organic layers. Mean δ15N values of NH4+ and NO3- were very similar in the drainage water at 90 cm soil depth at both sites (-7.1 to -3.8‰). A dynamic N cycling model was used to test the sensitivity of the natural abundance values for the amount of N deposition, the 15N ratio of atmospheric N deposited and for the intrinsic isotope discrimination factors associated with N transformation processes. Simulated δ15N values for the N saturated ecosystems appeared particularly sensitive to the 15N ratio of atmospheric N inputs and discrimination factors during nitrification and mineralization. The N-saturated coniferous forest ecosystems studied were not characterized by elevated natural 15N abundance values. The results indicated that the natural 15N abundance values can only be used as indicators for the stage of nitrogen saturation of an ecosystem if the δ15N values of the deposited N and isotope fractionation factors are taken into consideration. Combining dynamic isotope models and natural 15N abundance values seems a promising technique for interpreting natural 15N abundance values found in these forest ecosystems.