RESUMO
The biopolymer composition, immunotropic and immunogenic properties of the fractions of B. pseudomallei and B. mallei were under study. The first two capsular fractions of these agents were found to be similar in their biopolymer composition that was indicative of their close relations. At the same time the causative agents of glanders proved to have decreased content of high molecular glycoproteids and LPS fragments. In the causative agents of melioidosis, capsular fractions K3 and K4 were characterized by the domination of proteins with a molecular weight of 42-25 kD. Fraction K4 in B. pseudomallei and fraction K1 in B. mallei had pronounced immunosuppressing properties ensuring the protection of encapsulated microbial cells in the body. The biopolymers forming fractions K1, K2, K3 in B. pseudomallei and fraction K2 in B. mallei were characterized by immunomodulating properties.
Assuntos
Cápsulas Bacterianas/imunologia , Burkholderia pseudomallei/imunologia , Burkholderia/imunologia , Antígenos de Bactérias/imunologia , Cápsulas Bacterianas/química , Burkholderia/química , Burkholderia/patogenicidade , Burkholderia pseudomallei/química , Burkholderia pseudomallei/patogenicidade , Mormo/imunologia , Mormo/microbiologia , Glicoproteínas/análise , Terapia de Imunossupressão , Lipopolissacarídeos/análise , Lipopolissacarídeos/imunologia , Melioidose/imunologia , Melioidose/microbiologia , Peso MolecularRESUMO
When studied in vivo (in guinea pigs) with the use of electron microscopy, B. mallei (strains C-5, 10230) were found to form a capsule. In the subacute course of infection, the encapsulated forms of B. mallei parasitized mainly in the cells of the system of mononuclear phagocytes in the liver, the spleen and the lungs. The capsule formed by B. mallei was shown to be one of the factors facilitating its persistence in the body.
Assuntos
Cápsulas Bacterianas/biossíntese , Burkholderia/patogenicidade , Doença Aguda , Animais , Cápsulas Bacterianas/ultraestrutura , Burkholderia/ultraestrutura , Mormo/microbiologia , Mormo/patologia , Cobaias , Fígado/microbiologia , Fígado/ultraestrutura , Pulmão/microbiologia , Pulmão/ultraestrutura , Microscopia Eletrônica , Fagócitos/microbiologia , Fagócitos/ultraestrutura , Baço/microbiologia , Baço/ultraestruturaRESUMO
The results of the molecular biological detection of the etiologic agent of hemorrhagic fever in Rostov Province are presented. The role of the causative agents of Astrakhan rickettsial fever, hemorrhagic fever with the renal syndrome, Q fever, leptospirosis and listeriosis has been excluded by means of such immunochemical reactions as the direct and indirect immunofluorescent tests, the solid-phase immunoenzyme assay, the complement fixation test and the agglutination test. The relationship between the cases of hemorrhagic fever in the focus of the outbreak and Crimean-Congo hemorrhagic fever virus has been demonstrated due to the use of the polymerase chain reaction with preliminary reverse transcription.
Assuntos
Surtos de Doenças , Vírus da Febre Hemorrágica da Crimeia-Congo/isolamento & purificação , Febre Hemorrágica da Crimeia/diagnóstico , Anticorpos Antivirais/sangue , Especificidade de Anticorpos , Sequência de Bases , Primers do DNA , Diagnóstico Diferencial , Vírus da Febre Hemorrágica da Crimeia-Congo/genética , Vírus da Febre Hemorrágica da Crimeia-Congo/imunologia , Febre Hemorrágica da Crimeia/epidemiologia , Febre Hemorrágica da Crimeia/etiologia , Humanos , Dados de Sequência Molecular , Reação em Cadeia da Polimerase/métodos , Federação Russa/epidemiologia , Testes Sorológicos/métodosRESUMO
The results of the epidemiological analysis of the outbreak of hemorrhagic fever which was caused by Crimean-Congo hemorrhagic fever virus and occurred during the period of July 3-19, 1999, in the Oblivskaya district of Rostov Province are presented. The specific epidemiological features of the outbreak have been determined. The possible versions of the appearance of the focus of infection and the role of Ixodes ticks in the circulation of the infective agent are discussed.
Assuntos
Surtos de Doenças , Febre Hemorrágica da Crimeia/epidemiologia , Adolescente , Adulto , Distribuição por Idade , Distribuição de Qui-Quadrado , Criança , Pré-Escolar , Surtos de Doenças/estatística & dados numéricos , Reservatórios de Doenças/estatística & dados numéricos , Feminino , Febre Hemorrágica da Crimeia/diagnóstico , Febre Hemorrágica da Crimeia/etiologia , Febre Hemorrágica da Crimeia/transmissão , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Federação Russa/epidemiologia , Distribuição por Sexo , Fatores de TempoRESUMO
Phosphatase, phospholipase C and a proteolytic complex, including casein- and hemoglobin-hydrolases, have been isolated from cell-free extracts of B. pseudomallei cultivation medium. A set of monoclonal antibodies (McAb) to the antigenic complexes of this infective agent has been obtained. Conditions for the study of the interaction of the enzymes and McAb have been worked out, and most of the 14 immunoglobulins under study have been shown to neutralize 2-3 hydrolases each. Three types of McAb, selectively inhibiting only the proteolytic complex, only casein-hydrolase and only phospholipase C, have been identified.
Assuntos
Anticorpos Monoclonais/farmacologia , Burkholderia pseudomallei/enzimologia , Inibidores Enzimáticos/farmacologia , Hidrolases/farmacologia , Imunoglobulinas/farmacologia , Animais , Burkholderia pseudomallei/imunologia , Burkholderia pseudomallei/patogenicidade , Interações Medicamentosas , Hibridomas/imunologia , Hidrolases/antagonistas & inibidores , Hidrolases/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos BALB CRESUMO
An in vivo comparative study on penetration of free gentamicin sulfate and liposome-entrapped gentamicin sulfate to macrophages was performed and the efficacy of the treatment of destructive pneumonia was estimated on albino mice. The liposome-entrapped antibiotic was arrested by the cells of the mononuclear phagocytic system thus providing higher concentrations of the drug in the organs with high counts of macrophages, the antibiotic retention time in the organs being longer than that after the use of free gentamicin. The use of liposome-entrapped gentamicin in the treatment of destructive pneumonia made it possible to increase the host protection from 17 to 50 per cent.
Assuntos
Gentamicinas/administração & dosagem , Macrófagos Peritoneais/efeitos dos fármacos , Pneumonia Estafilocócica/tratamento farmacológico , Absorção , Animais , Permeabilidade da Membrana Celular , Portadores de Fármacos , Gentamicinas/farmacocinética , Lipossomos , Macrófagos Peritoneais/metabolismo , Camundongos , Resultado do TratamentoRESUMO
The paper summarizes the data concerning the production and study of monoclonal antibodies (MAb) to the diagnostically significant glanders and melioidosis bacillus antigens. It evaluates the efficiency of using MAb in the gel immunodiffusion and agglutination tests as a basis of new-generation preparations for fluorescent antibody assay, indirect hemagglutination test which are used while detecting and identifying pathogenic pseudomonads. The paper defines the quality indices for monoclonal luminescent immunoglobulins and provides evidence for the benefits of monoclonal diagnostic agents over polyclonal analogues.
Assuntos
Anticorpos Monoclonais/isolamento & purificação , Burkholderia pseudomallei/isolamento & purificação , Imunoglobulinas/isolamento & purificação , Pseudomonas/isolamento & purificação , Testes de Aglutinação/métodos , Testes de Aglutinação/normas , Testes de Aglutinação/estatística & dados numéricos , Animais , Burkholderia pseudomallei/imunologia , Hibridomas/imunologia , Imunodifusão/métodos , Imunodifusão/normas , Imunodifusão/estatística & dados numéricos , Indicadores e Reagentes/normas , Camundongos , Camundongos Endogâmicos BALB C , Pseudomonas/imunologiaRESUMO
The work presents the results of the study (carried out by the method of continuous flow cytofluorometry) of changes in the distribution of lymphocytes and their populations (obtained by means of distributing cell electrophoresis) according to the phases of the cell cycle (G0 + G1; S; G2 + M) in the blood and spleen of guinea pigs, as well as in the blood of humans, before and after immunization with cholera vaccine. The results of the determination of DNA-synthesizing lymphocytes in the blood of immunized humans and animals have been shown to serve as an objective characteristic for the complex evaluation of the biological activity of cholera vaccines.