The first seroepidemiological study on Toxoplasma gondii in backyard pigs in Myanmar.

A cross-sectional study of Toxoplasma gondii infection in pigs was carried out in backyard farms in three townships, within Nay Pyi Taw area from June 2014 to August 2014. Blood samples were randomly collected from 256 pigs in 129 farms. Using commercial Latex Agglutination Test kits, specific antibodies to T. gondii were analyzed. Based on LAT results, among 256 serum samples examined, 47 samples (18.4%) were found positive to T. gondii. The numbers of samples showing specific antibody titres from 47 positive pig sera were 20 at 1:64, 2 samples at 1:128, 9 samples at 1:256, 3 samples at 1:512 and 13 samples at 1:1024. Among the hypothesized risk factors, roaming of cats around the farm was found associated to T. gondii seropositivity in pigs (OR = 3.13; 95% CI = 1.33-7.34). This study provides information on seroepidemiology study of T. gondii in backyard pigs for the first time in Myanmar. This information will be useful in developing strategies for the control of T. gondii infection in pigs.

Serological Survey and Factors Associated with Toxoplasma gondii Infection in Domestic Goats in Myanmar.

Goat farming is important for the livelihood of millions of rural people because it contributes to food security and creation of assets. However, infection of goats with Toxoplasma gondii could be a source of parasite transmission to humans. The information on T. gondii infection of goat was not reported yet in Myanmar. A total of 119 goat serum samples were collected from three cities in the central region of Myanmar for T. gondii antibody survey. With the occurrence value obtained in this first study, a second one, more complete, with larger number (162) of animals and properties, was carried out and the risk factors and prevalence were determined. In both studies the samples were analyzed by the LAT. Of these, 32 (11.4%) samples were showed to be positive. The infection was associated with the presence of cats at the farm (odds ratio [OR] = 4.66, 95% confidential interval [CI] = 1.03-21.06), farming with different animal species (sheep, cattle, and pigs) (OR = 4.33, 95% CI = 1.57-11.94), and farming without good management practices (OR = 0.23, 95% CI = 0.06-0.83). This is the first T. gondii prevalence study in goats in the country.

Epidemiological Survey on Porcine Cysticercosis in Nay Pyi Taw Area, Myanmar.

Cross-sectional surveys were conducted to determine the prevalence and associated risk factors of Taenia solium cysticercosis in pigs within Nay Pyi Taw area, Myanmar. Meat inspection in three slaughterhouses, ELISA test, and questionnaire surveys were conducted in this study. Three hundred pigs were inspected in slaughterhouses and 364 pigs were randomly selected and examined from 203 households from three townships in Nay Pyi Taw area. The prevalence of porcine cysticercosis in meat inspection was 23.67% (71/300). Seroprevalence of T. solium cysticercosis in pigs in the study area was 15.93% (58/364). Significant associated risk factors with T. solium cysticercosis were gender (OR = 3.0; 95% CI = 1.7-5.4), increased age (OR = 2.3; 95% CI = 1.2-4.2), husbandry system (OR = 5.1; 95% CI = 2.4-11.2), feed type (OR = 16.9; 95% CI = 2.3-124.3), not using anthelmintics in pigs (OR = 11.9; 95% CI = 5.0-28.5), not using anthelmintics in owner (OR = 2.5; 95% CI = 1.4-4.4), no hand-washing before feeding (OR = 31.5; 95% CI = 4.3-230.9), and pork consumption of owner (OR = 37.4; 95% CI = 9.0-156.1) in the study area. This is the first report of porcine cysticercosis in Myanmar.

Composition and contamination of oral rehydration solutions prepared with well water by village mothers in Burma.

A field study was carried out at Htaukkyant village in Burma to assess whether village mothers could use condensed milk tins to measure one litre of water with reasonable accuracy for the preparation of oral rehydration solutions (ORS) and whether the extent of bacterial contamination of well water was serious and if this affected the bicarbonate content of the ORS solution. Empty condensed milk tins have a fairly uniform volume around 330 ml and using three condensed milk tins full of water mothers made up one litre quite consistently. Mothers also proved capable of preparing ORS solution by dissolving one packet of oral rehydration salt (ORS) in three condensed milk tins full of water to obtain solutions which contained acceptable and safe concentrations of sodium and potassium. Contamination of well water with faecal coliforms was present. Both storing water in domestic vessels and boiling water reduced the coliform count. Storing could be a good way of reducing the risk of infection if repeated contamination from dipping in to the water could be avoided. The counts on coliforms and faecal coliforms in ORS both increased by about 1 log per day over the first and second 24 hours after the preparation with contaminated well water. Despite this the bicarbonate content of ORS remained stable. In the absence of boiled water, ORS solution can be made using the cleanest available water and using it within 24 hours.